大鼠MMSCs异基因移植后的生存和对受体免疫状况的影响

目的 探讨骨髓间充质干细胞(MMSCs)同种异基因移植后的生存状况和对受体免疫状态的影响.方法 SD大鼠20只分为MMSCs移植组10只,培养液对照组10只.体外DiL荧光标记MMSCs,移植后共聚焦荧光显微镜下追踪MMSCs在体内的存活情况,观察受体鼠生长状况和眼部情况,比较两组角膜免疫分子MHC-Ⅱ mRNA的表达差异.结果 移植4周后大量的MMSCs存活于角膜缘,结构完整,生长良好.受体鼠全身情况良好,眼局部无排斥反应发生.受体鼠角膜表达MHC-Ⅱ,对照组未表达.结论 局部同种异基因移植后4周MMSCs在受体眼大量生存,受体全身和眼局部正常,应用于治疗眼表创伤疾病是可行的.     

准分子激光原位角膜磨镶术后角膜扩张

准分子激光原位角膜磨镶术(1aser in situ keratomileusis，LASIK)后角膜扩张是近年才认识的LASIK术后的一种并发症，其发生率不高，但可导致患视力严重损害，且不可逆转。本对其发病机制、组织病理学、临床特征和治疗等进行综述。     

大切口无缝线白内障(囊外)摘除术后泪膜变化的研究

Objective To investigate the alterations of tear film after sutureless large incision manual cataract extraction (SLIMCE). Methods SAw-eight eyes undergone SLIMCE operation were chosen ran-domly. Slit-limp microscope, break- up time (BUT), schirmmer Ⅰ test (Sit), FL were used to observe the alter-ations of tear film at different time points post-opcration. Impression cytology and microphoto-analyses tech-niques were also applied to observe the goblet cells at different time points post-operation. Results Subjective complaint of dry eye within 3 months after the operations were significantly increased compare with pre-opera-tions. The schirmmer Ⅰ test was greatly increased 2 weeks post operation,the mean scores of fluorescence in-creased and the mean break-up time decreased 1 month post operation (P <0.05 ). The impression cytology de-tection showed that the number of conjunetiva goblet cells in surgery area is lower at different time point post-operation respectively (29.62±4.31 ; 22.69±4.71 ; 39.05±4.27; 44.83±3.63; 45.76±3.55)than that in pre-operation (46.35±4.86 ) (P <0.05 ). Conclusion SLIMCE operation has effect on the stability of tear film.     

大切口无缝线白内障(囊外)摘除术后泪膜变化的研究

Objective To investigate the alterations of tear film after sutureless large incision manual cataract extraction (SLIMCE). Methods SAw-eight eyes undergone SLIMCE operation were chosen ran-domly. Slit-limp microscope, break- up time (BUT), schirmmer Ⅰ test (Sit), FL were used to observe the alter-ations of tear film at different time points post-opcration. Impression cytology and microphoto-analyses tech-niques were also applied to observe the goblet cells at different time points post-operation. Results Subjective complaint of dry eye within 3 months after the operations were significantly increased compare with pre-opera-tions. The schirmmer Ⅰ test was greatly increased 2 weeks post operation,the mean scores of fluorescence in-creased and the mean break-up time decreased 1 month post operation (P <0.05 ). The impression cytology de-tection showed that the number of conjunetiva goblet cells in surgery area is lower at different time point post-operation respectively (29.62±4.31 ; 22.69±4.71 ; 39.05±4.27; 44.83±3.63; 45.76±3.55)than that in pre-operation (46.35±4.86 ) (P <0.05 ). Conclusion SLIMCE operation has effect on the stability of tear film.     

雷帕霉素纳米粒滴眼液联合环孢素A缓释膜治疗兔高危角膜移植术后免疫排斥反应的研究

Objective To evaluate the combined effect of topical rapamycin (RAPA) eye drop in nanometer vector and poly (lactic acid) (PLA) wafers of cyclospoirne A(CsA) in the prevention of acute allograft rejection after rabbit corneal transplantation. Methods It was an experimental study. RAPA was incorporated into the nanometer particles and CsA was incorporated into PLA wafers. A was syngeneic control whose both donor and recipient are New Zealand rabbit. Gray donor corneas were implanted into the 102 recipients of New Zealand albino rabbits with corneal neovascularization who were randomly divided into B, C, D, E, F, G 6 groups to receive the different types of therapy: B was no therapy control; C was eye drop of nanometer vector but no RAPA twice a day,28 days; D was PLA wafers in the anterior chamber of rabbit eyes but no drugs; E was 0.5% RAPA eye drop of nanometer vector twice a day,28 days; F was PLA wafers of CsA in the anterior chamber of rabbit eyes; G was PLA wafers of CsA in the anterior chamber of rabbit eyes and 0.5% RAPA eye drop of nanometer vector eye drop twice a day for 28 days together. Postoperative evaluation included slit-lamp biomicroscopy, histopathology and immunohistoiogy, Cytokines related with neovascularization and immunosuppression in the corneal tissue by RT-PCR. The graft survival was assessed by One-Way ANOVA and q test. Results Corneal allograft survival time: A (100.00±0.00), B ( 8.44±1.24), C (8.89±2.57), D (8.56±2.30), E (43.11±5.58), F (43.67±9.54), G (72.00±15.34)d. Group G led to a statistically significant prolongation of transplant survival and was superior than group E and F which was a statistical prolongation compared with group B, C and D (qGE=11.42, qGF=11.24,qEB=13.64, qEC=13.38, q<=13.46, qFB=13. 82, qFC=13.56, qFD=13.64; P<0.01). Immunohistopathologically, the grafts were subjected to an immune response contained a dense infiltrate of neutorphils,CD4+ and CD8+ T lymphocytes in the group B ,C and D. This cellular infiltrate was a significant reduction in group E,F,G. RT-PCR showed that the gene expression of IL-2 was inhibited earlier (3 days) in group F, G and VEGF gene expression being suppressed later (14 days) in group E, G. Conclusions Combined therapy with topical application of RAPA eye drop of nanometer vector and CsA PLA wafers can significantly prolong the survival of allograft at high-risk. Moreover, topical combined treatment of them is more effective, lower dosage, less side-effects and cheaper than the treatment with topical individual immunosuppressive drug.     

Pro370Leu突变型MYOC基因真核表达质粒的构建及其表达特点

目的：构建Pro370Leu突变型MYOC基因真核表达质粒，并在Hela细胞中表达以研究蛋白的定位和分泌特点。方法：以pGEM—T—MYOC质粒中MYOC为模板，用PCR介导的定点突变技术，得到Pro370Leu突变型MYOC基因（mMYOC），并定向亚克隆到真核表达质粒pDsRed2-N1上，得到pDsRed2-N1—mMYOC重组表达质粒，再用限制性内切酶消化和DNA测序鉴定，最后用脂质体包埋转染法转染Hela细胞，进行荧光显微镜观察，并用Western Blot分析蛋白分泌情况。结果：经酶切和DNA序列测定，证实重组质粒构建成功，mMYOC基因能在Hela细胞中表达，并且蛋白只定位在细胞质中，经Western Blot分析，mMYOC蛋白不能分泌到培养液中。结论：成功构建Pro370Leu突变型MYOC基因真核表达质粒pDsRed2-N1—mMYOC，并能有效表达，其表达的蛋白定位在细胞质，并且不能分泌。     

帕金森病合并剂峰异动患者低频振幅的静息态fMRI研究

目的:观察帕金森病(Parkinson's disease,PD)合并剂峰异动患者的静息态脑活动特点,并探索其发病机制。方法:招募2017年3月至2019年11月就诊于南京医科大学第一附属医院的PD伴剂峰异动患者(剂峰异动组)27例,PD不伴异动患者(非异动组)29例和来自社区的健康对照27例。采集静息态功能磁共振(r...     

角膜地形图仪与曲率计测量角膜散光的对比观察

目的：对使用角地形图仪与角膜曲率计测量角膜散光结果进行对比研究。方法：对角膜散光小于１．５Ｄ的患者１１３名１３２眼,使用美国Ａｌｃｏｎ公司的ＥＹＥＭＡＰ＾ＴＭＥＨ－２９０角膜地形图仪和瑞士ＨＡＡＧ ＳＴＲＥＩＴ ＢＥＲＮ角膜曲率计测量角膜散光。结果：角膜地形图仪测得角膜曲率显著大于角膜曲率计测得相应值,两种仪器测得的角膜散光无显著差异,散光轴向符合率随散光度增大而增加。结论：角膜地形图系统测量角膜散光更准确、全面、尤其对角膜过陡、过平或有不规则散光的患者更可信。     

大切口无缝线白内障(囊外)摘除术后泪膜变化的研究

Objective To investigate the alterations of tear film after sutureless large incision manual cataract extraction (SLIMCE). Methods SAw-eight eyes undergone SLIMCE operation were chosen ran-domly. Slit-limp microscope, break- up time (BUT), schirmmer Ⅰ test (Sit), FL were used to observe the alter-ations of tear film at different time points post-opcration. Impression cytology and microphoto-analyses tech-niques were also applied to observe the goblet cells at different time points post-operation. Results Subjective complaint of dry eye within 3 months after the operations were significantly increased compare with pre-opera-tions. The schirmmer Ⅰ test was greatly increased 2 weeks post operation,the mean scores of fluorescence in-creased and the mean break-up time decreased 1 month post operation (P <0.05 ). The impression cytology de-tection showed that the number of conjunetiva goblet cells in surgery area is lower at different time point post-operation respectively (29.62±4.31 ; 22.69±4.71 ; 39.05±4.27; 44.83±3.63; 45.76±3.55)than that in pre-operation (46.35±4.86 ) (P <0.05 ). Conclusion SLIMCE operation has effect on the stability of tear film.     

湖北省综合性三级医院男护士工作倦怠状况调查

目的了解综合性三级医院男护士工作倦怠状况及其人口学影响因素。方法通过方便取样法，应用中文版工作倦怠量表（CMBI）及自制一般资料调查表对湖北省10家综合性三级医院的190名男护士进行调查。结果190名男护士的倦怠检出率为50．5％（96人），其中轻度倦怠占36．8％（70人）、中度倦怠占12．6％（24人）、高度倦怠占1．1％（2人）；倦怠单因素检出率中，人格解体占27．4％（52人），耗竭占25．3％（48人），成就感降低占12．6％（24人）；除不同编制的男护士之间耗竭维度得分差异有统计学意义（F=5．537，P〈0．05）外，其他不同人口学特征男护士之间的各倦怠维度得分差异无统计学意义（P〉0．05）。结论综合性三级医院一半以上男护士存在一定程度的工作倦怠，主要表现为人格解体及耗竭；不同编制对男护士耗竭程度有影响。