去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

多巴胺引起类似尿崩症状态一例

患者,男性,16岁,因确诊先天性心脏病、Fallot四联征,在体外循环直视下行心内矫治术。术后因出现心脏低排状态,血压降至10.0/5.0kPa,测中心静脉压:0.588～1.47kPa,间断静脉注射西地兰、地塞米松,同时多巴胺120mg加入5％葡萄糖溶液中持续静脉滴注,使血压维持在13.3/9.3kPa,用多巴胺后2小时,尿量明显增多,每小时达600～700ml,不伴明显口渴感。测血钾、钠在正常范围,二氧化碳结合     

果胶降脂胶囊治疗高脂血症的临床疗效观察

本文研究了37例原发性高脂血症病人在正常膳食条件下,每日口服12粒果胶降脂胶囊,治疗3周后血脂变化情况.结果表明:对血清甘油三酯和血清胆固醇有显著降低作用(P<0.05).在实验剂量条件下,未见果胶对胃肠功能有任何不良影响.本研究为临床开展高脂血症的防治提供了新的途径.     

酒精型气雾杀虫剂的研制及对蚊蝇蟑螂药效观察

目的 :研制一种药效高、气味好、耐腐蚀的气雾杀虫剂 ;方法 :分别测试实验室、模拟现场、现场、贮存稳定性、耐压、耐腐蚀性、毒性试验结果 ;结果 :实验室按 1.0 g/筒喷雾 ,对蚊蝇的 K T50 均小于 1.0分钟 ,对德国小蠊 K T50 为 6.3分钟 ,三种害虫的 2 4h死亡率均达 10 0 ;模拟现场 ,对蚊蝇和德国小蠊分别按 0 .3 g/m3 和5 .0 g/m2的剂量喷雾后 ,三种害虫 1h击倒率和 2 4h击倒率均为 10 0 ;现场试验按 0 .3 g/m3剂量喷药后 ,蚊蝇 10分钟内全部击倒 ,2 4h死亡率均达 10 0 ;药液贮存两年后药效分解率小于 5 ,耐压、耐腐蚀性显示正常 ;毒性鉴定属实际无毒级 ,对眼结膜轻度充血 ,对皮肤无刺激性。结论 :该气雾杀虫剂用食用酒精作溶剂 ,在两年内使用可替代煤油制剂     

防损伤套管在空心钉微创内固定术中的应用

以微创的术式，用空心钉治疗股骨颈骨折已有较多的报道。我院在术中使用一种自制的专利器械，能使同类型手术所致的创伤降低到最小程度，术中出血量减小到最少程度。现将我院1999～2003年治疗的58例情况报道如下。     

高龄食管癌贲门癌的外科治疗

高龄食管癌贲门癌在临床上并非少见，但由于考虑到患者的体质、家庭和社会因素，使不少患者失去最佳手术治疗时机。我院自1993年9月至2003年9月共手术治疗食管癌贲门癌67例，其中70岁以上13例，占19．4％，手术疗效满意，现报道如下。     

妊娠期肝内胆汁淤积症309例临床分析

妊娠期肝内胆汁淤积 (ICP)是妊娠期特有的并发症 ,被公认为是对胎婴儿威胁较大的高危妊娠之一 ,其主要危害是引起早产 ,羊水胎粪污染 ,胎儿宫内窘迫及围产儿死亡。本文总结 30 9例ICP孕妇及围产儿情况 ,现报告如下。对象与方法1　对象　 1997～ 2 0 0 1年我院住院分娩孕妇 ,符合ICP诊断标准 30 9例。2　方法　入院孕妇常规空腹抽血 ,检测肝功能、血胆酸等指标。血胆酸正常值范围 0～ 2 6 1μg/dL。将 30 9例孕妇按血胆酸值分级 ,A组 (≥ 30 0 0 μg/dL ,6 1例占 19 7% ) ,B组(10 0 0 30 0 0 μg/dL ,119例占 38 5 % ) ,C组 (≤ 10 0 …     

安徽医科大学出版中心主任黄河胜的祝贺信

十分高兴地获悉您主编的《临床肺科杂志》入选中国科技核心期刊，成为中国科技部论统计与分析的源期刊，标志着贵刊达到了新水平、取得了新进步。这是您和您领导的编辑部全体同仁坚持不懈、共同努力的结果。我谨代表安徽医科大学出版中心向您和编辑部全体同志表示衷心的祝贺!