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人体是否感染乙型肝炎病毒(HBV),除检测HBsAg外,检查乙型肝炎表面抗体(抗-HBs)也是重要手段之一。据估计,全世界至少有3/4 HBV携带者集中在亚洲、大洋洲地区,我国地处高发区,为保障人民健康,预防乙型肝炎的流行,开展乙型肝炎的流行病学研究和防治,已成为极有价值的课题,抗HBs是一种宿主对HBV感染后产生免疫能力的保护性抗体,因此,检测我国自然人群中抗HBs,从流行病的角度讲有其重要意义。为此,我们建立了放射免疫PEG法快速检测抗-HBs的方法,对各种影响因素进 相似文献
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放射免疫分析法(Radioimmunoassay,简称RIA)是1960年首先由Yalow和Berson创立的一种体外的超微量分析方法,它是将具有高灵敏度的放射性核素示踪技术与高度特异性的免疫化学技术二者结合而建立起来的新的分析方法。几乎一切 相似文献
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Intravenous supplement of essential amino acids (EAA) 12.6 g/d, fortified with branched-chain amino acids, was given to Group A, 5 patients with chronic renal failure (CRF) (mean creatinine clearance rate 6.55±2.49 ml/min) for 14 days on the basis of low protein diet (0.6 g/kg/d) , and Group B, 4 patients with CRF (mean creatinine clearance rate 9.75±3.93 ml/min) for JO days on the basis of low protein diet(0.4g/kg/d). The nitrogen balance, whole body nitrogen flux and rates of protein synthesis and catabolism and amino nitrogen utilization were markedly improved as compared with the pre-treatment levels. With treatment, the levels of some EAA in plasma such as valine, isoleucine, phenylalanine, lysine and methionine were increased, while that of tyrosine was not changed. In 4 of the Group A patients, blood urea nitrogen, creatinine and phosphate were slightly decreased after treatment, while serum calcium level was significontly increased. In 8 patients uremic symptoms were markedly ameliorated and their nutritional status apparently improved. 相似文献
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