金荞麦的致突变和抗突变研究

本文用Ames法对金荞麦的致突变和抗突变性进行了研究。结果表明:金荞麦在0.001mg/皿—5mg/皿7个剂量未发现阳性突变反应,并对正定霉素和甲基甲烷磺酸酯所诱发TA98和TA100菌株的突变,具有抗突变的作用。     

INTERACTION OF COMPONENT (E) WITH TUMOR CELLULAR DNA

Some antitumor activities of component (E), extracted from the root of Fagopynum Cymosum (Trev) Meisn (FCTM), have recently been discovered in vivo and in vitro. The component E (CE)'s pattern of action with tumor cellular DNA at the molecular pharmacological level was investigated by macromolecular synthesis experiment (MSE) and human DNA interaction system established in our laboratory. The experiments demonstrated that, in vitro, the agent could markedly inhibit the incorporation of 3H-TdR into the cellular DNA, and the IC50 in P388 leukemia cell and in SGC-7901 cell was 17.86 μg/ml and 110.4 μg/ml, respectively. The agent, at mg/ml level, could produce an intercalation reversion pattern with DNA within a short time (2 hours). But when the interval was prolonged for over 4 hours, the action changed to intercalation irreversible pattern. According to these observations, the authors infer that CE interacts with DNA in two ways - directly and indirectly. The indirect action, especially in low concentr     

金荞麦E抗肿瘤及抗炎作用的研究

Po、ip金荞麦E均能显著抑制小鼠S-180 u-14及Lewis肺癌等肿瘤的生长,Po给药对小鼠S-180、u-14及Lewis肺癌的最大抑瘤率分别为56.44％、48.22％、55.48％,但Po金荞麦E不能延长带白血病P388小鼠的存活时间。金荞麦E付二甲苯诱起的小鼠耳部炎症及甲醛溶液引起的小鼠踝关节肿均有明显抗炎作用。金荞麦(Fagopyrum Cymosum Meissn)为蓼科荞麦属植物,金荞麦E即从植物金荞麦中提得的一鞣质类化学物质。我们对金养麦E进行了药理活性及毒理试验的研究。本文主要报道金荞麦E抗肿瘤、抗炎的药理作用。     

金荞麦E对小鼠网状内皮系统吞噬功能的影响

本文报道用印度墨汁法测定金荞麦E对小鼠网状内皮系统吞噬功能的影响。正常小鼠PO金荞麦E200、100、50mg/kg/day,测得小鼠吞噬指数K分别为0.026±0.008、0.024±0.007、0.021±0.008,吞噬系数α分别为5.335±1.048、5.095±1.047、5.061±1.164,与对照组比较(对照K值0.014±0.009,α值4.057±0.837)100、200两组K值、α值均显著提高。荷瘤小鼠PO金荞麦E200、100mg/kg/day,K值分别为0.047±0.0098、0.0521±0.0096,α值分别为6.5557±0.5154,6.8298±0.9750,与对照组比较(对照K值     

金荞麦E药理作用的研究

金荞麦E即为金荞麦成份之一。实验结果表明:金荞麦E能显著抑制小鼠肉瘤S_(180),子宫颈癌u-14及Leuis肺癌等肿瘤的生长,但不能延长带白血病P_(388)小鼠的存活时间。同时金荞麦E抗瘤活性的有效剂量范围大,PD给药更为明显。P.o金荞麦对S_(130)、u-14、Leuis肺癌的最大抑瘤率     

金荞麦的致突变性和致畸胎性研究

本文报道中草药荞麦的致突变性和致畸性试验的结果。金荞麦1-5000μg/皿时,对TA97,TA98,TA100和 TA102在±S9的条件下,均未诱发阳性结果;对正定霉素和甲基甲烷磺酸酯所诱发的TA98和TA100菌株的回复突变,具有抗突变作用;对NIH系小鼠骨髓嗜多染红细胞微核试验结果阴性;未见诱发中国仓鼠卵巢细胞(CHO)染色体(±S9)畸变的作用;对NIH系小鼠的生殖能力和胎鼠的生长发育均未见不良影响,对胎鼠外观、骨骼、内脏亦无致畸胎作用。