Graves''''眼病患者眼眶前脂肪细胞抵抗素诱导的表达

目的通过检测抵抗素在Graves’眼病患者眼眶前脂肪细胞培养分化过程中的表达,进一步研究前脂肪细胞的分化过程。方法在体外通过组织块培养法获取前脂肪细胞,前脂肪细胞在分化液的作用下转化为成熟脂肪细胞。利用RT-PCR法检测前脂肪细胞及成熟脂肪细胞中抵抗素mRNA的表达情况,对前脂肪细胞及成熟脂肪细胞进行免疫组化检测抵抗素蛋白的表达情况。结果前脂肪细胞中无抵抗素mRNA及蛋白的表达。成熟脂肪细胞中有抵抗素mRNA及蛋白的表达。免疫组化结果显示抵抗素蛋白位于成熟脂肪细胞胞浆中。结论Graves’眼病患者眼眶组织中存在功能活跃的前脂肪细胞,能够在体外分化为脂肪细胞。抵抗素是脂肪细胞分泌的一种脂肪细胞因子,仅存在于成熟脂肪细胞中,可作为脂肪细胞分化的标记物。     

眼眶海绵状血管瘤的CT和彩色多普勒超声诊断分析

目的：探讨眼眶海绵状血管瘤的CT和彩色多普勒超声定位定性诊断意义。方法：对1986～2000年住院的214例眼眶海绵状血管瘤病人进行CT和超声波检查。结果：CT检查诊断符合率为72％，CT可做出准确的眼眶内病变定位，其中75％的肿物位于肌椎内。超声波检查对眼眶海绵状血管瘤的定位不如CT，但对揭示海绵状血管瘤的组织学类型优于CT，其诊断符合率高达93％。结论：用CT扫描作为术前精确定位，以决定手术入路与切白位置，结合CT与超声波作术前准确的定性诊断以决定是否采用简单的前路开眶摘除肿物具有重要的实际指导意义。     

直肌联结术治疗水平直肌麻痹

目的　观察直肌联结术治疗水平直肌麻痹的手术效果。方法　回顾分析从 1 993年 7月～1 996年 1 2月采用直肌联结术联合同侧内直肌或外直肌后退矫正 1 2例 1 4眼近完全或完全性水平直肌麻痹的手术效果。随访时间 2个月到 5年 ,平均 2年。结果　一次手术后正位 9例 1 1眼 ,1例过矫6°,1例欠矫 7°,1例经三次手术仍欠矫 1 0°伴轻度上斜视 ,即合并出现了垂直性偏斜。全部病例外观满意 ,外转 (外直肌麻痹者 )与内转 (内直肌麻痹者 )功能部分恢复 ,固视野恢复 1 2°～ 3 0°,平均2 0°,未发现眼前节缺血并发症。结论　直肌联结术治疗水平直肌麻痹效果理想 ,文中对水平直肌麻痹的手术方法、眼前节缺血、手术效果等进行了讨论     

双目间接检眼镜下激光治疗眼底病

双目间接检眼镜下激光治疗眼底病金陈进胡兆科高汝龙吴中耀梁美玉激光手术是治疗许多眼底病的有效方法，近两年来，我们应用双目间接检眼镜氩激光治疗了部分眼底病，报告如下。１对象与方法１．１对象本组患者共３２例、３６只眼，男２２例，女１０例，年龄最小２个月，最...     

超声生物显微镜在眼球前段肿瘤检查中应用的初步报告

目的:探讨超声生物显微镜(Ultrasound biomicroscope,UBM)在眼前段肿瘤诊断的价值。方法:用UBM对13例临床诊断或疑诊的眼球前段肿物患者进行检查。结果:3例有外伤或手术史、临床诊断为前房植入性虹膜囊肿,UBM显示有较大的无内反射的囊性回声;8例前房较浅、房角较窄、怀疑虹膜后或睫状体肿物中,7例显示虹膜后有大小不等的囊性回声(其中2例有多个囊性回声),1例睫状体部有实性肿物回声;2例临床诊断为虹膜痣者,显示虹膜实性隆起或稍增厚、均匀一致的高回声影。结论:UBM检查有助于眼球前段肿瘤的诊断和定位。眼科学报1997;13:189—191。     

端粒酶抑制剂对葡萄膜黑色素瘤细胞生长的抑制作用

Objective To study the restrain effects of two telomerase inhibitors included IFN-α2b (interferon-α2b)and CDDP(cisplatin;cis-dichlorodiamine platinum)to uveal melanoma cells of human eyes,and to provide theory bases for chemotherary of uveal melanoma.Methods Applied IFN-α2b and CDDP to the primary cultured uveal melanoma cells of human eyes individually with different concentration and duration,and compared with control group.Morphological changes were observed by inverted optical and electronic microscope,and the restrain effects were detected by MTT.Results More and more morphological changes of restraint and apoptosis of cells were observed with the increasing concentration and duration of IFN-α 2b and CDDP.When action durations were 72 h.the cells were inhibited with the concentration more than 500 IU/ml(IFN-α 2b)and 1.00 mg/L(CDDP)respectively.The cells restrain rates were(66.1±4.7)%and(74.0+3.3)%with the concentration more than 5000 IU/ml(IFN-α2b)and 10 mg/L (CDDP),and the rates were higher than before(P<0.001).When concentrations were 5000 IU/ml(IFN-α2b)and 10.00 mg/L(CDDP),cells were inhibited at 48 h and the restrain rates were(67.9±8.1)%and(76.5±1.61%at 72 h,respectively,(P相似文献   

端粒酶抑制剂对葡萄膜黑色素瘤细胞生长的抑制作用

Objective To study the restrain effects of two telomerase inhibitors included IFN-α2b (interferon-α2b)and CDDP(cisplatin;cis-dichlorodiamine platinum)to uveal melanoma cells of human eyes,and to provide theory bases for chemotherary of uveal melanoma.Methods Applied IFN-α2b and CDDP to the primary cultured uveal melanoma cells of human eyes individually with different concentration and duration,and compared with control group.Morphological changes were observed by inverted optical and electronic microscope,and the restrain effects were detected by MTT.Results More and more morphological changes of restraint and apoptosis of cells were observed with the increasing concentration and duration of IFN-α 2b and CDDP.When action durations were 72 h.the cells were inhibited with the concentration more than 500 IU/ml(IFN-α 2b)and 1.00 mg/L(CDDP)respectively.The cells restrain rates were(66.1±4.7)%and(74.0+3.3)%with the concentration more than 5000 IU/ml(IFN-α2b)and 10 mg/L (CDDP),and the rates were higher than before(P<0.001).When concentrations were 5000 IU/ml(IFN-α2b)and 10.00 mg/L(CDDP),cells were inhibited at 48 h and the restrain rates were(67.9±8.1)%and(76.5±1.61%at 72 h,respectively,(P相似文献   

光动力疗法对实验性视网膜母细胞瘤细胞植瘤超微结构的影响

目的：评价血卟啉衍生物（HPD）介导的光动力疗法（PDT）对裸鼠眼内视网膜母细胞瘤（KB）移植瘤超微结构的影响。方法：将15只裸小鼠视网膜细胞瘤眼内动物模型眼分成A、B、C、D及E五组,其中A和B组为观察组,即既给光敏剂HPD又照光,但光照剂量不同,A组为150mw/cm^2,B组为300mw/cm^2;C组不给光敏剂,只照光;D组只给光敏剂HPD,不照光;E组为未作任何处理的对照组。血卟啉衍生物剂量为5mg/kg,经尾静脉注射给药。照光时机为光敏剂注射后72小时。照光仪的绿激光小为532nm。照光后24小时摘除眼球作透射电镜检查。结果：①实验组A：肿瘤细胞结构不完整,胸质呈浓缩状。核膜不完整,核染色质浓缩成块或裂解成碎片;②实验组B：肿瘤细胞破坏程度更明显,看不到完整的细胞轮廓,也见不到膜性结构及细胞器,仅见一些呈浓缩状态的片状物质,见不到完整的细胞核。③对照组E组：未见组织或细胞损害。瘤细胞轮廓清晰,胞膜完整。胞质内有丰富的线粒体、粗面内质网、核糖体。核膜完整,核间隙未见明显增宽。C组的D组瘤细胞超微结构的改变状况同E组。结论：本研究结果提示：血卟啉衍生物介导的光动力作用对裸鼠眼内RB移植瘤具有显著的杀伤作用,高照光剂量下肿瘤细胞坏死程度更明显。     

兔眼玻璃体内注射足叶乙苷对视网膜神经节细胞的毒性作用

目的 观察兔眼玻璃体内注射足叶乙苷对视网膜神经节细胞(RGC)的毒性作用.方法 采用随机数字法将24只健康新两兰大白兔随机分为正常组和0.0、1.5、15.0、150.0、375.0、750.0、1500.0μg 足叶乙苷组,每组3只兔,每只兔双眼用于实验.正常组不作任何处理,其余各组兔眼玻璃体内分别注射0.0、1.5、15.0、150.0、375.0、750.0、1500.0 μg足叶乙苷.注射后12 d,空气栓塞法处死所有兔.取眼球作常规病理检查,苏木精-伊红(HE)染色,光学显微镜观察视网膜形态变化、计数视网膜颞侧距视盘颞侧缘1 mm处400倍视野的RGC数量.取距视盘1 mm处颞上方1 mmX 1 mm大小视网膜行常规透射电子显微镜检查,观察视网膜超微结构变化情况.结果 光学显微镜和透射电子显微镜观察发现,正常组兔眼视网膜结构清晰规整,RGC呈球形或类球形;0.0、1.5、15.0、150.0μg足叶乙苷组兔眼视网膜各层结构与正常组相似;375.0、750.0、1500.0μg足叶乙苷组兔眼视网膜明显变薄,层次结构紊乱.正常组、0.0、1.5、15.0、150.0、375.0、750.0、1500.0μg足叶乙苷组兔眼RGC计数分别为(61.66±3.72)、(61.83±4.59)、(60.00±7.07)、(62.00±4.43)、(62.67±3.98)、(7.33±1.37)、0.00、0.00个,8组间差异有统计学意义(F=145.04,P=0.00).0.0、1.5、15.0、150.0μg足叶乙苷组兔眼RGC计数较正常组无明显变化,差异无统计学意义(F=0.244,P＞0.05);375.0μg足叶乙昔组兔眼RGC计数较正常组、0.0、1.5、15.0、150-0 μg 足叶乙昔组明显降低,差异有统计学意义(F=145.04,P＜0.05).结论 足叶乙苷对兔眼RGC有一定毒性作用,且该作用呈剂量依赖性.

Abstract：

Objective To observe the toxic effects of intravitreal injection of etoposide on retinal ganglion cells (RGC) in rabbit eyes. Methods Twenty-four healthy rabbits were divided into 8 groups randomly, including one normal group without any treatment, and 7 experimental groups with intravitreal injection of different dosages of etoposide (0.0, 1.5, 15.0, 150.0, 375.0, 750.0, 1500.0 μg). Twelve days after the injection, the animals were killed by air embolism, and the eyeballs were prepared for routine pathological examination with hematoxylin-eosin staining and transmission electron microscopy. Results Four experimental groups (0.0, 1.5, 15.0, 150.0 μg etoposide) had normal retinal structure and normal shape of ganglion cells while other 3 experimental groups with higher dosage of etoposide (375.0, 750.0 and 1500.0 μg) showed thinner and disorganized retina. The number of RGC of the normal group and 0.0, 1.5,15.0, 150.0, 375.0, 750.0, 1500.0 μg groups were 61.6±63.72, 61.83±4.59, 60.00±7.07, 62.00±4.43, 62.67±3.98, 7.33±1.37, 0.00 and 0.00 per 400 X visual field, with statistical difference among groups (F=145.04, P = 0.00). There was no statistical difference between the normal group and 0.0,1.5, 15.0, 150.0 μg groups (F=0.244,P＞0.05). The number of RGC of 375.0 μg group was decreased obviously compared with normal group and 0.0, 1.5, 15.0, 150.0 μg groups with statistical difference (F=145.04, P＜0.05). Conclusion Etoposide has a dosage-dependent toxicity on rabbit RGCs.     

TSHr在Graves眼病患者眼眶脂肪细胞中的表达

目的探讨Graves眼病患者眼眶脂肪细胞中TSHr的表达。方法应用RT—PCR技术对8例Graves眼病患者、8例无甲亢和眼部免疫性炎症因其他病变需行眼球摘除的病人的眼眶脂肪细胞中的TSHr表达情况进行了检测。结果Graves眼病患者眼眶脂肪细胞中可检测到较强的TSHrmRNA表达，而正常对照组眼眶脂肪细胞中仅见较弱的TSHrmRNA表达，其表达的差异有显著性（P=0．001）。结论这些结果支持TSHr是甲状腺、眼眶共享抗原之假说。