2005年我院抗感染药物在传染病使用中的调查分析

为了对三级甲等传染病医院抗感染药物使用情况有一个较全面的了解，进一步地合理使用抗感染药物，避免滥用抗感染药，我们对一所三级甲等传染病医院2005年使用抗感染药物患者进行回顾性调查分析。     

肿瘤患者临床应用抗菌药物的回顾性分析

目的 了解肿瘤患者应用抗菌药物情况,为规范使用抗菌药物提供相关数据.方法 调查2010年4月-2011年4月肿瘤住院患者2671份病历使用抗菌药物情况,并进行回顾性分析.结果 抗菌药物使用率肿瘤一科为31.5％、肿瘤二科为38.85％、肿瘤三科33.46％、肿瘤四科为49.08％,抗菌药物药敏送检率为84.62％;结论 肿瘤手术患者机体自身抵抗力低,且感染因素较复杂并且抗菌药物不合理使用现象普遍存在,需加强抗菌药物应用的监管,尤其是预防用药,应针对存在的问题,进一步规范管理,促进抗菌药物合理使用.     

海藻糖和蛋黄在人类精子冻于保存中的保护作用

人类精子冻干保存中损伤除了来自环境溶液对精子生理活性的损伤外，还会来自于冷冻和干燥过程。目前精子冻干保存中保护剂选取基本上都从生理化学角度考虑，目的为抑制精子内酶活性，没有考虑对冷冻和干燥损伤的抑制。大量文献表明蛋黄能减小冷冻对精子的损伤，而二糖（特别是海藻糖）在细胞干燥中具有特殊保护作用。本研究在前人一般使用的精子冻干保护剂中加入海藻糖，或同时加入海藻糖和蛋黄，通过伊红Y染色、低渗膨胀和电镜显微观测实验，比较不同组成的保护剂对精子结构和膜的保护作用。结果表明，通过加入海藻糖修正的保护剂能提高对精子结构的保护效果，当同时加入海藻糖和蛋黄时效果更好。     

我院高危药品管理的实践与探索

在借鉴 ISMP 和我国医院药学专业委员会制定的“高危药品分级管理策略及推荐目录”的基础上,我院从高危药品的品种确立、养护、使用以及信息化管理、人员培训等方面进行了实践和探索,取得了较好成效,降低了高危药品不当使用带来的医疗风险。     

厚朴酚对抑郁模型大鼠海马神经可塑性的影响

目的研究厚朴酚对抑郁模型大鼠海马神经可塑性的影响及其相关机制。方法 SD大鼠采用慢性温和不可知应激(CUMS)制备抑郁模型,ig给予不同剂量的厚朴酚(20、40 mg/kg),阳性对照组给予氟西汀(20 mg/kg),共给药28 d。观察各组大鼠自发活动、强迫游泳时间、糖水偏好程度3项行为学指标;荧光定量PCR检测各组大鼠脑内海马、皮质、纹状体区域的微管相关蛋白-2(MAP-2)、神经生长相关蛋白-43(GAP43)、突触素(SYP)的m RNA表达;免疫组化法检测大鼠海马区域MAP-2、GAP43、SYP蛋白表达水平;免疫印迹法检测各组大鼠脑内海马区域MAP-2、p-MAP-2及磷酸化细胞外信号调节激酶(p-ERK)蛋白表达水平。结果模型组大鼠自发活动减少、糖水偏好降低、强迫游泳不动时间延长,造模成功。与模型组比较,厚朴酚各剂量可以显著提高大鼠自发活动能力,增加慢性应激大鼠糖水消耗量,减少慢性应激大鼠在强迫游泳中的不动时间(P0.05、0.01);提高慢性应激所致的大鼠海马区MAP-2 m RNA及蛋白水平的降低(P0.05);促进大鼠脑内海马区p-MAP-2蛋白表达(P0.05),显著提高p-ERK的表达(P0.05)。结论厚朴酚可以通过ERK通路影响MAP-2的磷酸化,增加MAP-2的表达,从而影响神经元的可塑性,发挥其抗抑郁作用。     

corilagin protects non-alcoholic fatty liver disease in mice induced by high fat and high sugar diet via regulating AMPK-autophagy signaling北大核心CSCD

Aim To explore the effects of corilagin on non-alcoholic fatty liver disease induced by high-fat and high-sugar diet in mice via regulating AMPK-autophagy signaling. Methods Healthy 8-week-old male C57BL/6J mice were randomly divided into control group, model group and corilagin group. The mice of model group and corilagin group were fed with a high-fat and high-sugar diet for four weeks at the age of eight weeks. The corilagin group mice were also intraperitoneally injected with corilagin (20 mg • k g - 1 ), which was given once every 2 days for 4 weeks. The mice of the control group and the model group were given equal dose of normal saline. After modeling and administration, the mice were sacrificed and the liver weight recorded. The liver pathological changes of each group mice were assessed by HE staining, oil red O staining and Masson staining. The biochemical indexes in serum and liver tissue were detected by the ELISA kit. The p-AMPK and autophagy levels were detected by Western blot. Results The results showed that compared to control group, the liver weight of the model group increased, the AST and ALT levels in serum also significantly increased, there were a large number of fat vacuoles and severe lipid deposition and mild collagen fibrosis in liver, while the liver weight and TG level in liver significantly decreased, and the liver pathological changes were significantly improved after treated with corilagin. Western blot results showed the levels of autophagy related proteins such as Atg7 and Atg5 significantly decreased in the model group, and the p-AMPK level also significantly decreased. When treated with corilagin, p-AMPK and the autophagy levels were up-regulated. Conclusion corilagin can protect non-alcoholic fatty liver disease in mice induced by high fat and high sugar diet. The mechanism may involve increasing p-AMPK level and enhancing autophagy level in liver. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

水飞蓟宾导致慢性肝炎患者缺铁性贫血的研究

目的：研究水飞蓟宾对慢性肝炎患者缺铁性贫血的影响。方法：选取120例慢性肝炎患者分为两组（每组60例）,两组患者在性别、年龄、临床类型及肝功能等实验室检查指标上均无显著性差异。对照组服用多烯磷脂酰胆碱（易善复,赛诺菲安万特制药有限公司）,剂量为70~140 mg,口服,每日3次,12周为一个疗程;试验组服用水飞蓟宾（水林佳,天津天士力制药有限公司）,剂量为70~140 mg,口服,每日3次,12周为一个疗程。两组于治疗前、治疗2周、治疗结束后4周测定血红蛋白、血清铁蛋白、血清铁。结果：对照组60例中,服药前后,血红蛋白、血清铁蛋白、血清铁均无显著变化;试验组60例,给药后血红蛋白、血清铁蛋白、血清铁的差异有显著性意义（P<0.05）。结论：水飞蓟宾会导致慢性肝炎患者缺铁性贫血的发生。