心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

甲状腺手术中甲状旁腺损伤原因分析及功能保护

目的:探讨甲状腺手术中甲状旁腺的损伤原因及功能保护措施。方法:回顾分析行甲状腺手术319例患者的临床资料,150例(原位保护组)术中行甲状旁腺探查显露原位保护法;162例(传统手术组)按传统方法切除甲状腺。结果:原位保护组中有2例(1.33%)术后出现暂时性甲旁减;20例(13.33%)血钙水平明显降低;58例(38.67%)术后血钙水平一过性降低。而传统组中分别为8例(4.94%)、64例(39.51%)、66例(40.74%)。暂时性甲旁减和血钙水平明显降低两组比较差异有统计学意义。无永久性甲状旁腺功能减退,无死亡病例。行甲状腺部分切除、单侧叶次全切除、单侧叶全切除、双侧叶次全切除、双侧叶全切、双侧叶全切及颈淋巴结清扫者,暂时性甲旁减发生率依次为0%、0%、0%、1.23%、1.23%、3.79%。结论:甲状旁腺的功能保护取决于手术者的强烈的保护意识、扎实的解剖知识、娴熟的操作技巧、术中对其血供的充分保护及术后对其血管痉挛的积极预防。在术中充分解剖显露原位保护甲状旁腺,能够大大降低术后甲状旁腺功能减退的发生率。     

TFF3在贲门肠化组织中的蛋白表达及其与癌变的相关性

目的: 探讨TFF3蛋白在贲门肠化及肠化伴不典型增生组织中的表达与贲门癌变的相关性.方法: 采用免疫组织化学SP法, 检测河南贲门癌高发区贲门炎组织24例、肠化组织52例、肠化伴不典型增生组织65例及贲门癌组织35例中TFF3蛋白的表达变化.结果: 在贲门炎及肠化组织中, TFF3蛋白主要表达在柱状细胞的纹状缘, 肠化伴不典型增生及癌变组织中, 主要表达在细胞质中.TFF3蛋白在贲门炎、肠化组织、肠化伴不典型增生及癌变组织中的阳性表达率分别为20.8%、44.2%、46.2%和54.3%, 贲门黏膜组织从正常至不典型增生再至癌变这一过程与TFF3表达阳性率呈正相关( r = 0.232, P<0.05).TFF3蛋白表达的位点与病理类型相关(Kappa= 0.490).结论: TFF3蛋白在细胞质中的过表达参与了贲门癌变过程, 有可能成为贲门组织早期癌变的预警分子.     

（MMTV）-ErbB-2转基因鼠孕期使用三羟异黄酮对子代小鼠乳腺肿瘤中血管内皮生长因子的影响

目的观察三羟异黄酮（genistein）对Erb-2转基因小鼠乳腺肿瘤发生的影响。方法采用高剂量：15mg／（kg·d）、中剂量：5．0mg／（kg·d）、低剂量：1．0mg／（kg·d）三羟异黄酮对孕期第13～19天小鼠皮下注射,观察其子代雌性小鼠和对照组子代雌性小鼠乳腺肿瘤的潜伏期和乳腺成瘤率是否存在差异,并用免疫组织化学染色SP方法检测子代小鼠乳腺肿瘤组织VEGF的表达情况。结果高剂量组乳腺癌肿瘤潜伏期、乳腺成瘤率与其它各组存在显著差异性（P〈0．05）。高剂量组VEGF的阳性表达水平明显低于其他各组（P〈0．05）。结论高剂量异黄酮孕期的使用能够延长子代雌性小鼠肿瘤发生的潜伏期,降低乳腺成瘤率,降低子代小鼠乳腺肿瘤中VEGF的阳性表达水平。     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

目的 观察经心肌梗死边缘区移植骨髓间充质干细胞(MSCs)24 h和2周后的全身分布.方法 雄性SD大鼠的MSCs用溴脱氧尿苷(BrdU)标记.将雌性SD大鼠经心梗3周后随机分为2组.第1组为标记细胞(3×10,50μl)经心肌注入心梗边缘区(n=12).第2组为等体积的磷酸盐缓冲液(PBS)经心肌注入心梗边缘区(n=8).移植细胞24 h及2周后,体内细胞的分布情况通过实时定量逆转录-聚合酶链反应(RT-PCR)检测及病理切片免疫组织化学染色来评价.结果 细胞经心梗边缘区移植后可以分布在心脏以及心外脏器脾脏、肺脏和肝脏中.移植24 h后细胞主要在心脏中分布(467 467±191 387),其他脏器中的分布较少,而在2周后移植细胞在包括心脏(112 388±43 751)在内的各个脏器中的分布都很少,病理切片免疫组织化学染色结果显示与RT-PCR结果相符.结论 MSCs经心梗边缘区移植后主要在心脏中分布,同时脾脏、肺脏、肝脏中也有分布,但其分布数量随着移植时间锐减.     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

神经妥乐平治疗含紫杉醇方案化疗所致周围神经损伤的疗效观察

<正>在肿瘤内科进行化疗的患者中,出现周围神经损伤是较常见的不良反应之一,很多化疗药物都可引起,如长春新碱、紫杉醇、奥沙利铂等。近年来较常用的新药奥沙利铂等均能引起,患者多数表现为四肢末端麻木及感觉异常,严重的还会导致肢体功能障碍而致化疗中断或延迟。紫杉醇是一种新的抗微管药物,其作用机制是能特异的结合到微管的β位上,导致微管聚合成团块和束状使其稳定,这些作用能抑制微管的正常重组。神经妥乐平是近年来应用比较广泛的一种药     

心肌梗死后移植骨髓间充质干细胞后全身细胞的分布

Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs) 24 h and 2 weeks after cell injection into the border zone of myocardial infarction area. Methods MSCs from male SD rats were labeled with Bromodeoxyuridine (BrdU). Three weeks after in-duction of myocardial infarction,female SD rats were randomized into 2 groups. Labeled cells (3 × 10 ,50 μl) were injected into the border zone of infarcted area in the one group (n = 12) ,and PBS of equal vol-ume was injected into the border zone of infarcted area in the other group ( n = 8 ). The systemic distribu-tion of MSCs was evaluated through real-time PCR and immunohistochemistry at time points of 24 h and 2 weeks after injection, respectively. Results Cells injected into border zone of infarcted area were distribu-ted to extra-cardiac organs such as spleen,lung and liver. Twenty-four h after injection,cells mainly con-centrated in the heart (467 467 ± 191 387) ,while obvious cell loss was noted in all organs including the heart ( 112 388 ±43 751 ) 2 weeks after injection. Of immunostaining were consistent with those of real-time PCR. Conclusion After injected into the border zone of infarcted area, MSCs mainly gathered in the heart with distributions into spleen, lung, and liver. However, substantial number of cells lost with pro-longed time span.     

特发性乙状结肠破裂14例诊治体会

特发性乙状结肠穿孔又称乙状结肠自发性破裂，是指乙状结肠本身无任何病变或无外伤所致突然穿孔，继发弥漫性腹膜炎。本病临床上比较少见，我院于2001年7月至2008年10月共收治经手术及病理检查证实为乙状结肠自发性破裂14例，现分析报告如下。