oxLDL 对转人apo A1基因小鼠动脉平滑肌细胞胆固醇摄取和清除的影响

目的　探讨①oxLDL对转人apoA1C5 7BL/ 6小鼠主动脉SMC(trSMC)胆固醇清除与摄取的影响 ,以衡量转入的apoA1基因是否有助于减轻oxLDL引起的SMC胆固醇积聚 ;②oxLDL刺激下 ,转入的apoAI基因的表达状况 ,以探知在致病的环境下 ,所转入的基因是否能增加表达 ,提高抗病能力。方法　①用已建立的含小鼠金属硫蛋白Ⅰ (MT Ⅰ )启动子的人apoAI转基因C5 7BL/ 6小鼠主动脉SMC ,通过3H -胆固醇标记率和清除率测定 ,观察oxLDL对trSMC胆固醇摄取与清除的影响。②通过RT PCR及Northernblot技术检测oxLDL对trSMC基因表达的影响。结果　 30 μg/mloxLDL明显促进小鼠SMC增生 ;未见trSMC与nSMC在标记率间的差别 ,oxLDL刺激后标记率增加约 10 0 % ;trSMC清除率与nSMC相比 ,增加 4 0 % - 5 0 % ;oxLDL刺激后 ,nSMC清除率下降 2 8% ,而trSMC下降10 % ;RT PCR及Northernblot表明oxLDL刺激后trSMC基因表达明显增加。结论　转人apoA1基因小鼠SMC的基因表达有助于减轻oxLDL引起的SMC胆固醇积聚 ;在oxLDL刺激下 ,trSMC的人apoA1基因表达增加 ,减轻了oxLDL的致病作用。     

Molecular basis for the effect of lipid lowering drugs on growth factors after de-endothelialization

Objective To study the mechanism and effect of lipid lowering drugs in arresting the development of arterial restenosis after angioplasty.Methods De-endothelialization injury of rabbit aortae, common iliac and femoral arteries using balloon angioplasty and the expression of growth factors such as platelet derived growth factor-B (PDGF-B), transforming growth factor β-1 (TGFβ-1), and fibroblast growth factos (bFGF) were investigated. Total serum cholesterol (TC) and triglycerides (TG) were analyzed during and after the treatment using either simvastatin combined with gemfibrozil or simvastatin alone for 6 weeks. Results Serum total cholesterol and triglycerides were only slightly to moderately increased after high cholesterol ration intake lasting for 6 weeks in rabbits of two therapeutic groups (simvastatin plus gemfibrozil or only simvastatin). A positive correlation was found between TC and intimal/medial ratio (r=0.5873, P<0.05). PDGF-B detected by immuno-histochemistry and RT-PCR analysis showed that the release of PDGF-B was inhibited by simvastatin and gemfibrozil after de-endothelialization. RT-PCR analysis showed that TGFβ-1 was increased in the neointima in two treatment groups but no definite change was seen in the mRNA of bFGF in the smooth muscle cell (SMC) of the balloon-injured arteries even under lipid lowering drug treatment. Conclusion In addition to the lipid lowering effect, both simvastatin and gemfibrozil also influence the release of PDGF-Band TGF-1 in the neointima after de-endothelialization.     

A study of apolipoprotein A-IV genetic polymorphism, serum lipids and lipoproteins in Beijing habitants.

ＡｓｔｕｄｙｏｆａｐｏｌｉｐｏｐｒｏｔｅｉｎＡ┐ＩＶｇｅｎｅｔｉｃｐｏｌｙｍｏｒｐｈｉｓｍ，ｓｅｒｕｍｌｉｐｉｄｓａｎｄｌｉｐｏｐｒｏｔｅｉｎｓｉｎＢｅｉｊｉｎｇｈａｂｉｔａｎｔｓＷａｎｇＺｈｏｎｇｙａｎ王中燕，ＳｈｅＭｉｎｇｐｅｎｇ佘铭鹏，ＸｕＭａ...     

心血管系统病理学回顾与展望

心血管系统病理学回顾与展望王泰龄，佘铭鹏，宋继谒４０年来心血管系统的病理研究取得很大进展，通过协作，总结大量人体材料，揭示了我国一些心血管病如动脉粥样硬化和肺心病的特点，在数量上超过国外的统计。近２０年来随着医学生物学的发展及新技术的应用，心血管的实...     

载脂蛋白A-Ⅳ的代谢和功能

载脂蛋白 A-Ⅳ(apoA-Ⅳ)是一种酸性糖蛋白,分子量46kD,主要由小肠和肝脏合成分泌。apoA-Ⅳ可能的功能有:①激活卵磷脂胆固醇酰基转移酶;②辅助 apoC-Ⅱ激活脂蛋白脂酶;③apoA-Ⅳ的脂质体促进细胞内胆固醇外流;④apoA-Ⅳ能与细胞结合。因此,apoA-Ⅳ可能在脂质代谢,特别是在胆固醇逆转运过程中起重要作用。     

应用基因重组和基因转移技术抑制血管平滑肌细胞增生的研究进展

应用基因重组和基因转移技术抑制血管平滑肌细胞增生的研究进展梁红佘铭鹏平滑肌细胞增生是动脉壁对刺激的反应。动脉内膜平滑肌细胞增生和表型转换是动脉粥样硬化形态学的主要特征之一，在动脉粥样硬化的发生中起着极重要的作用。动脉再狭窄，严重地影响诸如经皮冠状动脉...     

树鼩主动脉内皮细胞株的建立及其生物学特性的研究

将树鼩胸主动脉分层剥离,用组织块贴壁法,体外培养出主动脉内皮细胞,历经一年,传至23代,命名为TSaec-8910。倒置显微镜下细胞单层生长,呈铺路石样镶嵌排列,第Ⅷ因子相关抗原阳性,透射电镜观察,细胞质内未找到Weibel-Palade小体。细胞生长曲线及分裂指数示9～12d汇合成单层,按1:2或1:3传代,传代间隔为lO～14d,细胞冻存复苏后接种存活率为31.5％,细胞染色体检查为二倍体细胞,2n=62,雄性。内皮细胞生长因子、上皮细胞生长因子、条件培养基和附着底物对TSaec-8910细胞有明显影响,细胞在玻璃瓶壁上贴壁时间为24～18h,而在涂有鼠尾胶瓶壁则为4h左右,内皮细胞生长因子、上皮细胞生长因子能促进TSaec-8910细胞贴壁和增殖,20％TSaec-8910细胞条件培养基亦能良好地维持细胞形态。     

第三次国际病理学术会议

国际病理学会(International Academy of Pathology)中国区分会和中华医学会病理学会第三次联合学术大会于1994年10月15～19日在广州召开。到会代表283人。会议共收到论文630篇,其中报告246篇。澳大利亚国际著名病理学家、下