脊柱浆细胞瘤中OPN和MMP-9蛋白的表达及临床意义

目的 探讨脊柱浆细胞瘤中骨桥蛋白 (osteopontin, OPN)和基质金属蛋白酶-9(MMP-9)的表达及其与脊柱浆细胞瘤生物学行为的关系.方法 应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结(S-P)法检测41例脊柱浆细胞瘤及14例脊柱良性单纯性骨囊肿中OPN和MMP-9蛋白的表达.结果 ①OPN在脊柱浆细胞瘤中的阳性表达率为82.93%(34/41),MMP-9蛋白阳性表达率为95.12%(39/41),而良性骨囊肿中OPN和MMP-9蛋白表达均呈全阴性,差异有统计学意义(P<0.01);②脊柱浆细胞瘤中OPN蛋白的表达程度在M蛋白分型和临床分型各组之间差异均有统计学意义(P<0.05).结论 ①OPN在脊柱浆细胞瘤患者的预后中可能具有保护作用.②联合检测OPN和M蛋白分型以及临床分型对脊柱浆细胞瘤的预后判断可能具有一定的临床意义.③MMP-9将在抗转移治疗靶点方面,为临床治疗提供一个新的选择.     

结直肠癌及其淋巴结转移灶内AnnexinⅠ和Annexin Ⅱ的表达及意义

结直肠癌的病死率位列癌症死亡的第4位,其发生、发展和转移的机制及临床病理分级备受关注.有学者认为膜联蛋白(Annexin)Ⅰ、Ⅱ与肿瘤的发生、发展和转移有关,可以作为提示预后的标志分子,但也有人持相反观点.本实验旨在研究AnnexinⅠ和AnnexinⅡ的表达情况与结直肠癌的病理分级和转移的关系.     

老年人阻塞性睡眠呼吸暂停综合症行鼻腔正压通气治疗的护理

对老年人阻塞性睡眠呼吸暂停综合征行鼻腔持续正压通气治疗的患者实施有针对性的护理及有效的健康宣教,对提高治疗效果有重要意义.     

神经生长因子基因转染联合强化铁营养防治豚鼠爆震性聋的实验研究

Objective To study the protective effects of adenovirus-mediated human beta-nerve growth factor gene (hNGFβ) transfer combined with iron fortified nutrition on blast hearing damage in guinea pigs. Methods Deafness was induced by blast (172dB SPL) in 35 healthy guinea pigs. Seven days after noise exposure, 10 guinea pigs were inoculated with adenovirus-mediated hNGFβ (Ad-hNGFβ) into the perilymphatic space (the gene group), another 10 guinea pigs were given hNGFβ combined with iron fortified nutritional diet (the combination group), still another 10 guinea pigs were inoculated with artificial perilymphatic fluid (APF), and 5 guinea pigs served as control, given neither medicine nor noise exposure. Auditory brainstem response (ABR) threshold shifts were monitored in the guinea pigs before blast exposure and after gene transfer. Changes in cochlear morphology were examined with immunohistochemical and HE staining. Results One week after successful inoculation of hNGFβ, it was noted that Ad-hNGFβ protein was expressed in each turn of the cochlea, and its intensity was almost equal. After gene transfer in the combination group and the gene group, ABR threshold shifts at week 1 and 4 were significantly smaller than those of the APF group. HE staining showed that the number of the spiral ganglion cells in the combination group and the Ad-hNGFβ group was significantly higher than that of the control group after 4 weeks (P<0.01). Conclusions Ad-hNGFβ combined with iron fortified nutritional diet was effective in the prevention and treatment of blast hearing damage of cochlear hair cells.     

脊柱转移癌新生血管形成与预后关系

目的 :探讨新生血管形成和脊柱转移癌预后之间的关系。方法 :应用免疫组化技术 ,检测 77例人脊柱转移癌组织血管内皮细胞生长因子 (vascular endothelial cell growth factor,VEGF)、VEGF受体 1(flm s- like tyrosine kinase,FL T)、VEGF受体 2 (kinase insertdomain containing receptor,FL K- 1)表达和微血管密度 (microvessels density,MVD)计数。采用 Spearm an等级相关判断 VEGF、FL K- 1、FL T表达之间及与 MVD计数之间的关系 ,以 COX回归分析判别 VEGF、FL K- 1、FL T表达与预后的关系。 结果 :VEGF、FL T、FL K- 1主要表达于转移癌细胞。新生血管形成多位于转移癌的侵袭性边缘。MVD计数 4 .2～ 5 6 .4 (2 1.6 3± 10 .95 )个。FL K- 1、FL T、VEGF表达和 MVD计数之间均呈显著正相关 (P<0 .0 1)。平均随访时间 15 .6个月(5 .2～ 36个月 ) ,3年生存率为 6 .98%。COX回归分析表明 VEGF、FL K- 1表达及 MVD计数为影响脊柱转移癌患者预后的重要因素 (P<0 .0 5 ,P<0 .0 1) ,其中 VEGF表达上调对患者生存的影响最大。 结论 :新生血管生成对脊柱转移癌患者预后有重要影响     

TSHR在甲状腺相关眼病中表达的研究

目的:检测甲状腺相关眼病(thyroid-associated ophthalmopathy,TAO)患者球后组织和眼外肌中是否有促甲状腺激素受体(TSHR)蛋的表达。方法:选用27例TAO患者球后脂肪、结缔组织和眼外肌标本和4例正常脂肪、结缔组织及眼外肌标本,常规石蜡切片,3％H2O2灭活内源性过氧化物酶,pH 6.0柠檬酸缓冲液高压锅修复抗原,加兔抗人TSHR抗体4℃过夜,     

罗格列酮对胰岛素抵抗大鼠肾皮质内皮素1及内皮型一氧化氮合酶表达的影响

目的研究罗格列酮对胰岛素抵抗(IR)大鼠肾皮质内皮素1(ET-1)、内皮型一氧化氮合酶(eNOS)表达的影响。方法高糖饲料喂养SD大鼠6周,复制IR大鼠模型。成模后用药组(RSG组)予罗格列酮10μmol·k-1·d-1灌胃,用药6周。用免疫组化、RT-PCR方法检测肾皮质ET-1及eNOS的蛋白和mRNA表达的改变。结果RSG组ET-1蛋白及mRNA显著低于IR组、eNOS蛋白及mRNA显著高于IR组,均P<0.01。结论在IR早期岁格列酮治疗可以明显减轻IR大鼠肾皮质ET-1与eNOS平衡的异常,保护肾血管内皮功能。     

Expression of hNav1.8 sodium channel protein in affected nerves of patientswith trigeminal neuralgia

Objective: To explore the pathogenesis of trigeminal neuralgia (TN) and to provide a new target for the drug treatment of TN by studying the expression of tetrodotoxin-resistant hNavl. 8 sodium channel protein in affected nerves of patients with TN. Methods: Twelve affected inferior alveolar nerves were obtained from patients with idiopathic TN, to whom the drug therapy was not effective. As negative control, one normal inferior alveolar nerve was obtained from patients who accepted the combined radical neck dissection with glessectomy and mandibulectomy. One muscle sample was obtained as normal control. One dorsal root ganglion from rat was as positive control. These tissues and prepared hNav1.8 antibody were conducted immunohistochemistry response. Results: hNavl. 8 channel protein was expresses in all the 12 specimens of the affected nerves of patients with TN, but not in the muscle sample and the normal inferior alveolar nerve. Conclusion: The abnormal expression of hNavl. 8 channel protein in the affected nerves of patients with TN may play an important role in the pathogenesis of TN.     

人胰腺褪黑素受体亚型基因及其蛋白表达的研究

人胰腺褪黑素受体亚型基因及其蛋白表达的研究     

治疗性沟通健康教育流程在颅脑损伤患者健康教育中的应用

目的探讨治疗性沟通健康教育流程对颅脑损伤患者健康教育的效果观察。方法将120例颅脑损伤患者按住院时间先后编号,按照随机数字表法随机分为对照组和实验组,每组各60例。对照组给予常规健康教育流程、实验组给予治疗性沟通健康教育流程,出院前对颅脑损伤患者进行评价。结果实验组的健康教育知晓率、患者治疗依从性、患者及家属对护理服务满意度均高于对照组(P0.05);实验组感染性并发症发生率低于对照组(P0.05),持续时间少于对照组(P0.05)。结论治疗性沟通健康教育流程能有效提高患者健康教育知晓率、患者治疗依从性,促进患者康复进程,提高患者及家属对护理服务的满意度。