Experimental study of the effect on the liver of cortisol after diverting the adrenal venous circulation into the portal circulation

In order to evaluate the operation of the adrenal autotransplantation into omentum with arterio-venous anastomosis, the right adrenal of fifteen dogs was preliminarily removed and then the left adrenal vein was diverted into the portal circulation by micro surgical technique. Before and after the diversion, the serum cortisol taken from portal vein, hepatic vein, inferior vena cava and peripheral vein was analysed by radioimmunoassay. The dog's adrenal cortex, pituitary were removed for light and electron-microscopic examination. The experimental results demonstrate that the cortisol inactivation effect of the liver was not statistically different before and after the procedure (P greater than 0.05). Thus, we believe that the omentum which has obvious absorbability and revascularity is a reasonable site for adrenal transplantation. And we also found the adrenal cortex left appear slight hypotrophy and pituitary hypertrophy after the diversion. The cause however, requires further research.     

两种根管弯曲度测量法间数学关系的推导

目的:研究2种根管弯曲度测量法测值(Schneider法的测值为θS,Weine法的测值为θW)间的数学关系。方法:将弯曲根管长轴简化为依次由相切的线段(长度为l1)、圆弧(半径为r、圆心角为θ)、线段(长度为l2)组成的连线,根据2测量法确定简化弯曲根管长轴测量角时不同的定点、连线方式,推导θS、θW间的函数方程以及与l1、l2、r、θ间的数学关系,分析方程与函数图形的特征。结果:θS与θW间存在以下数学关系:(1)tanθS=(1-cosθW ksinθW)/(sinθW kcosθW),k=l2/r;(2)θW/2≤θS≤θW。k取不同值时函数曲线的形态具有不同的特点。θW在0,π的区间内,θS与θW间呈近似的线性关系;k越大,θS越接近θW,k越小,θS越接近θW/2。结论:Schneider法与Weine法测值间存在着复杂的函数关系,根管根尖部直段的长度(l2)与弯曲段曲率半径(r)之比值是影响2种方法测值间差异的重要因素。     

Local cerebral blood flow responses in rats to hypercapnia and hypoxia in the rostral ventrolateral medulla and in the cortex.

The effects of hypercapnia and hypoxia on two local cerebral blood flows in the parietal cortex (PC-BF) and rostral ventrolateral medulla (RVLM-BF) were examined using laser Doppler flowmetry in anesthetized rats. Hypercapnia for 45 s duration at the end-tidal CO2 between 5% and 10%, induced by increasing inspiratory CO2, increased both cerebral blood flows and systemic blood pressure in a degree-dependent manner. The response of RVLM-BF was significantly stronger than that of PC-BF. Both cerebral blood flow responses to hypercapnia were not influenced by cutting peripheral chemoreceptor afferent nerves. Hypoxia for 45 s duration at the end-tidal O2 between 12% and 6%, induced by decreasing inspiratory O2, produced an increase of similar magnitude in both RVLM and PC local blood flows in a degree-dependent manner and a decrease in systemic blood pressure. The responses of both PC-BF and RVLM-BF to hypoxia were significantly diminished after cutting peripheral chemoreceptor afferent nerves. It is concluded that: (1) the RVLM-BF is much more sensitive to hypercapnia than the PC-BF; and (2) activation of peripheral arterial chemoreceptors possibly contributes to hypoxia-induced increase in the RVLM-BF and PC-BF.     

The Influence of Intramammary Lipopolysaccharide Infusion on Serum Ca,P, Vitamin D,Cytokines and Cortisol Concentrations in Lactating Sows

Ten multiparous lactating sows were used to investigate whether intramammary infusion of lipopolysaccharides (LPS; Escherichia coli 0111:B4; 2.0 μg/kg of body weight) would affect the circulating concentrations of Ca, P, 25‐hydroxyvitamin D (25‐OHD), tumour necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and cortisol. The sows were randomly allotted to either control group (control) or LPS‐treated group with five individuals per group and were infused with either physiological saline solution or LPS solution. The rectal temperature and udder quarter appearance were recorded at 0 (just before infusion), 1, 3, 7, 12 or 24 h after infusion. Blood samples were taken at 0, 1, 3, 7, 12 or 24 h after infusion. Before infusion, the rectal temperatures of all sows were below 39.2°C. At 3 and 7 h after infusion, the sows in the LPS group had a rectal temperature over 39.4°C. At 24 h after infusion, the rectal temperatures returned to pre‐infusion levels. Serum Ca and P concentrations in the LPS group decreased (P < 0.05) after LPS infusion compared with the control group at 1 h after infusion. No significant differences (P > 0.05) in the concentrations of 25‐OHD were observed between groups control and LPS at any sampling time. Increased (P < 0.01) concentrations of serum TNF‐α, IL‐6 and cortisol were observed in the LPS group compared with the control group at 3 and 7 h after infusion respectively. In conclusion, the elevation of serum concentrations of TNF‐α, IL‐6 and cortisol and the alterations of circulating concentrations of Ca and P following LPS infusion indicate that the immune system has been activated and immune activation may affect macromineral homeostatic regulation, which might have important implications for metabolic health of lactating sows. Lowered serum Ca and P following immune activation also shows a causative mechanism whereby immune activation increases the risk of secondary disorders such as mastitis‐metritis‐agalactia syndrome. However, immune activation did not affect circulating concentrations of vitamin D metabolites.     

帕金森病立体定向手术并发脑内出血的原因分析

目的:探讨帕金森病立体定向手术颅内出血并发症原因及对策。方法:自1999年4月至2003年3月我们对药物治疗效果不理想的510例帕金森病病人行立体定向毁损手术，术后出现颅内出血9例，其中毁损灶出血3例，穿刺道出血6例。结果:手术治疗4例．保守治疗5例。术后意识恢复但遗留偏瘫4例，经非手术治疗5例．恢复良好，未遗留明显功能障碍。全组无死亡病例。结论:采用磁共振结合微电极导向提高靶点定位精确度，减少微电极记录针道数，降低毁损温度，重视围手术期处理等措施，有助于降低颅内出血并发症的危险。     

Soluble polystyrene derivatives of metal bis(3,4-dicarboxybenzoyl)phthalocyaninates: Synthesis,thermal analysis,and sensitivity towards toxic gases

Polystyrene-bound metal [2,9 or 2,10 (or 2,16 or 2,17) bis(3,4-dicarboxybenzoyl)]phthalocyaninates were synthesized by Friedel-Crafts reaction of polystyrene with the corresponding metal phthalocyaninates. Co(II) and Cu(II) [2,9 or 2,10 (or 2,16 or 2,17) bis(3,4-dicarboxybenzoyl)]-phthalocyaninate (PS-CodaPc and PS-CudaPc) contained 0,13 mmol · g^?1 (12,4 wt.-%) and 0,13 mmol · g^?1 (12,8 wt.-%) of CodaPc and CudaPc, respectively. They were soluble in N,N'-dimethylformamide, but only partially soluble in chloroform, tetrahydrofuran (THF), dimethyl sulfoxide, N-methyl-2-pyrrolidone, and pyridine. The THF extracts contained 0,12 mmol · g^?1 (11,4 wt.-%) and 0,18 mmol ? g^?1 (17,2 wt.-%) of PS-CodaPc and PS-CudaPc, respectively. The thermal stability of the polymers was studied using thermogravimetric and differential thermal analysis in nitrogen and synthetic air atmosphere. The contents of MdaPc(M: metal) in THF-extracted polymers calculated from the data of residue in thermogravimetric analysis are 0,12 mmol · g^?1 for PS-CodaPc and 0,19 mmol · g^?1 for PS-CudaPc. In addition, the sensitive properties of the polymers towards toxic gases were also investigated by quartz microbalance transducers. The results show that the quartz microbalance sensors coated with both polymers were sensitive to NO₂ and chlorinated hydrocarbons, i.e. chloroform and perchloroethylene. The sensitivity to NO₂ was 6,53 · 10^?7 m³ · mL^?1 · s^?1 for PS-CodaPc and 1,90 · 10^?6 m³ · mL^?1 · s^?1 for PS-CudaPc, and that to chloroform and perchloroethylene was 2,33 · 10^?8 and 4,60 · 10^?8 m³ · mL^?1 · s^?1, respectively, for PS-CodaPc and 4,79 · 10^?8 and 9,51 · 10^?7 m³ · mL^?1 · s^?1 for PS-CudaPc.     

甾体避孕药不增加血栓栓塞性疾病危险(病例对照研究)

本文采用1:3病例对照研究方法,对甾体避孕药的使用与血栓栓塞性疾病(急性心肌梗塞、脑栓塞、脑血栓形成和深静脉血栓形成)间的关联进行多因素分析。结果提示在我国广泛应用的甾体避孕药(主要为国产Ⅰ号避孕药)并不增加血栓栓塞性疾病的危险性。用药和停药时间的长短与血栓栓塞性疾病的发生无关,并与高血压病之间亦不存在协同作用。     

Partial replication of a DRD4 association in ADHD individuals using a statistically derived quantitative trait for ADHD in a family-based association test.

BACKGROUND: Previous research found an association between single nucleotide polymorphisms (SNPs) in the promoter region of DRD4 and statistically derived phenotypes generated from attention-deficit/hyperactivity disorder (ADHD) symptoms. We sought to replicate this finding by using the same methodology in an independent sample of ADHD individuals. METHODS: Four SNPs were genotyped in and around DRD4 in 2631 individuals in 642 families. We developed a quantitative phenotype at each SNP by weighting nine inattentive and nine hyperactive-impulsive symptoms. The weights were selected to maximize the heritability at each SNP. Once a quantitative phenotype was generated at each SNP, the screening procedure implemented in PBAT was used to select and test the five SNPs/genetic model combinations with the greatest power to detect an association for DRD4. RESULTS: One of the four SNPs was associated with the quantitative phenotypes generated from the ADHD symptoms (corrected p-values = .02). A rank ordering of the correlation between each of the ADHD symptoms and the quantitative phenotype suggested that hyperactive-impulsive symptoms were more strongly correlated with the phenotype; however, including inattentive symptoms was necessary to achieve a significant result. CONCLUSIONS: This study partially replicated a previous finding by identifying an association between rs7124601 and a quantitative trait generated from ADHD symptoms. The rs7124601 is in linkage disequilibrium (LD) with the SNPs identified previously. In contrast to the previous study, this finding suggests that both hyperactive-impulsive and inattentive symptoms are important in the association.