Vitamin D receptor gene polymorphism in postmenopausal women of the Han and Uygur nationalities in China

Ｔｈｅｒｉｓｋｏｆｏｓｔｅｏｐｏｒｏｓｉｓｉｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｂｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙ (ＢＭＤ ) ,ｗｈｉｃｈｉｓｕｎｄｅｒｓｔｒｏｎｇｇｅｎｅｔｉｃｃｏｎｔｒｏｌ Ｍｏｒｒｉｓｏｎｅｔａｌ1　ｈａｖｅｓｈｏｗｎａｓｔｒｏｎｇｒｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｐｏｌｙｍｏｒｐｈｉｓｍａｔｔｈｅｖｉｔａｍｉｎＤｒｅｃｅｐｔｏｒ (ＶＤＲ)ｇｅｎｅａｎｄＢＭＤ ＩｎａｄｄｉｔｉｏｎｔｏＢｓｍⅠ ,ｒｅｓｔｒｉｃｔｉｏｎｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒｐｈｉｓｍａｔｔｈ…     

血管内皮生长因子165反义RNA治疗人脑胶质瘤的实验研究

目的 探讨应用血管内皮生长因子１６５（ＶＥＧＦ１６５）反义ＲＮＡ治疗人脑胶质瘤的可行性。方法 构建和鉴定反义ＶＥＧＦ１６５真核表达载体;将其转染入人脑胶质瘤细胞ＳＨＧ４４,检测其转染前、后的生物学性状;比较转染前、后ＳＨＧ４４细胞的裸鼠皮下致瘤性;分别应用免疫印迹、免疫组织化学、微血管计数、电镜和流式细胞仪检测上述改变。结果 成功构建反义ＶＥＧＦ１６５真核表达载体并在ＳＨＧ４４细胞获得表达,该细胞     

The prevalence of NIDDM and IGT and related factors among residents in some areas of Hubei Province, China

The epidemiological survey of prevalence of NIDDM (non-insulin dependent diabetes mellitus) and IGT(impaired glucose tolerance) was conducted among 9450 residents aged 25-70 in some areas of Hubei Province, China. The results show that NIDDM and IGT prevalences are 2.62% and 4.48%, respectively. There is no significant difference between male and female (P > 0.05). The NIDDM prevalence in cities is slightly higher than that in countryside, but the difference is not significant (P > 0.05). However, the IGT prevalence in city is significantly higher than that in countryside (P < 0.01). The prevalence of both NIDDM and IGT is increasing along with the age of the population. It is also significantly related to the family history of NIDDM, hypertension, and high body mass index (BMI). By using stepwise logistic regression to analyse the risk factors of NIDDM, age (OR = 1.86), BMI(OR = 2.69), family history (OR = 2.84) and hypertension (OR = 2.23) entered the model (significance level is alpha = 0.05).     

Adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose X-ray irradiation in mice

The dose-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by whole-body X-ray irradiation (WBI) was studied in male Kunming mice. The inductive doses (D1) were 25, 50, 75, 100 or 200 mGy 6 h before the challenging doses (D2) of 1.0, 1.5 or 2.0 Gy. The changes in the percentages of the thymocyte apoptotic bodies (TAB) and the cells in different phases of cell cycle were measured with flow cytometry. The percentages of TAB decreased, the arrests of G1 and G2 + M phases diminished, and the cells of DNA synthesis of S phase increased when the D1 + D2 groups was compared with the D2 groups. When D1 was 200 mGy, the adaptive response of thymocyte apoptosis and cell cycle progression were no longer induced by low dose radiation (LDR). In addition, the extracellular fluid from the splenocytes were cultured with Con A for 48 h in vitro 24 h after 75 mGy WBI was placed in the murine thymocyte suspension from mice irradiated with 2.0 Gy WBI and co-incubated. The thymocyte apoptosis decreased. Especially, noteworthy was that the percentages of TAB after the incubation for 72 h were significantly lower than those in 2.0 Gy irradiated thymocytes (P < 0.05). These results indicate that when the mice were irradiated with 25-100 mGy (D1, 12.5 mGy/min) 6 h before 1.0-2.0 Gy (D2, 0.287 Gy/min) exposure, an adaptive response of thymocyte apoptosis and cell cycle progression may be induced under the condition of WBI, and LDR (75 mGy) may change the microenvironment of immune cells and decrease the thymocyte apoptosis.     

Urban-rural comparison of HBV and HCV infection prevalence in eastern China

The present study was initiated to make and urban-rural comparison of the prevalence of cases positive to hepatitis B and C virus(HBV and HCV,respectively)infection markers in densely populated eastern half of China.For this purpose.10 survey sites were selected,i.e.,six sites in urban areas(the city group;Beijing,shangahi and four provincial capitals)and four sites in rural areas(the village group;one village each in Jilin and Shandong provinces,and two villages in Shaanxi Province),About 50 adult women per site volunteered to participate,from whom 494 valid bllod samples were collected.Positivities to HBsAg(HBsAg^ ),anti-HBs(anti-HBs^ )and antiHBc(anti-HBc^ )were examined by RIA methods.and that to anti-HCV(anti-HCV^ )by either EIA or RIA.Those positive to any one of the three HBV infection markers were taken as HBV infection-positive(HBV^ ).The prevalence of HBsAg^ ,HBV^ and anti-HBc^ was 8%,70%and 2.7% in the city group,and 8%,65% and 2.0%in the village group,and no significant difference was found between the two groups.The overall prevalence was 8% for HBsAg^ ,68% for HBV^ ,and 2.4% for anti-HVC^ ,The results were discussed in reference to some 20 papers each on HBV^ and anti-HCV^ prevalence in China published since(1991),The reviewing of these papers of anti-HCV was low(well below 5%),and that no substantial difference was found between the rural and urban populations.     

5-氨基水杨酸片剂的抗炎镇痛作用

目的　研究 5 -氨基水杨酸 (5 ASA)的主要药效学。方法　镇痛试验采用小鼠热板法及扭体法 ;抗炎试验采用小鼠耳片法、蛋清诱导大鼠足肿胀模型。结果　 5 ASA片剂 3种剂量 (75、15 0及 30 0mg·kg-1,ig× 3d)对二甲苯诱导的小鼠耳肿胀度及蛋清诱导的大鼠踝关节肿胀度均有明显抑制作用 ;以上剂量还可明显抑制小鼠扭体反应 ,大剂量还可延长小鼠热板的舔足反应潜伏期。结论　 5 ASA片剂有明显的抗炎镇痛作用。     

覆膜自膨式金属支架治疗恶性食管气管瘘

目的 探讨覆膜金属支架治疗恶性食管气管瘘的效果及并发症。方法 在Ｘ线引导下１４例食管气管瘘用国产覆膜支架治疗,门诊行食管造影或电话随访。结果 除１例瘘口封堵不全外,其余１３例瘘口完全封堵,未发生因操作引起的并发症。８例进普食,６例进半流食,１２例肺部感染得到控制。并发症：严重胸痛３例,支架移位致瘘再开放１例,颈动脉及左侧喉返神经受压致头晕及咽麻痹各１例。８例病人死亡,术后生存时间１．４～１２个月,     

慢性丙型肝炎自身免疫发病机制的研究

To explore the autoimmune pathogenesis of chronic hepatitis C virus(HCV) infection. Anti-GOR, antinuclear antibodies(ANA), thyroglobulin antibody(TGA), thyroid microsome antibody(TMA), serum levels of soluble Fas(sFas), and peripheral blood lymphocyte(PBMC) subsets and their apoptosis were measured in chronic HCV infection by using immunity assay and flow cytometry, respectively. The results showed that the positive rates of anti-GOR, ANA and TMA/TGA were significantly higher in chronic HCV-infected patients than those in normal controls(P < 0.01, respectively). In comparison with chronic HBV infected patients, anti-GOR and ANA were also significantly increased in chronic HCV infected patients(P < 0.01, P < 0.05, respectively). The serum levels of sFas were significantly higher in chronic HCV infection than those in healthy donors(P < 0.01). The apoptosis percentage of PBMCs and CD3+ cell was all increased in chronic HCV infection (vs normal controls P < 0.05). However, the apoptosis percentages of CD4+ T and CD19+ B cells in PBMCs were significantly decreased in patients with anti-GOR positive as compared with anti-GOR negative(P < 0.01, P < 0.05). The results indicate that autoimmune reactions and the imbalance of lymphocyte apoptosis exist during chronic HCV infection. Decreasing of the apoptosis of CD4+ T and CD19+ B lymphocytes may be the important reasons for the mechanism of autoimmune pathogenesis of chronic HCV infection. Increased serum levels of sFas may be responsible for the decrease of the apoptosis in a part of lymphocytes in chronic HCV infection.     

混配农药中氰戊菊酯在小鼠体内的代谢和分布

目的　了解混配农药中氰戊菊酯在小鼠体内的代谢和分布。方法　以14 C -氰戊菊酯为示踪剂 ,静脉注射给药。给药后 0 .5～ 12 0min之间采 9次血样 ,8～ 96 0min之间分别对脑、心脏、肝脏和肺脏采 8次样品。样品用 β闪烁计数仪测量 ,并换算成化学浓度。用残数法计算毒代动力学参数。结果　混配农药中的氰戊菊酯的代谢符合二室开放模型。分布相T1/ 2 α为 2 .7min ,消除相T1/ 2 β为 10 6 .6min ,表观分布容积为 2 .9L/kg ,正向扩散系数k12 (0 .17min-1)大于逆向扩散系数k2 1(0 .0 77min-1)。分布浓度肺脏中最高 ,其次为肝脏、心脏、脑。结论　肺脏优势参与了氰戊菊酯的代谢过程 ,应重视此类农药的肺脏毒性。     

南京城郊居民卫生服务需求调查分析

为了解南京市西部城郊社区居民卫生服务需求情况 ,我们对该地区 15岁以上居民 80 0 0人进行了问卷调查。结果显示 ,受调查人群两周患病率为 31 5 % ,两周就诊率 5 9 9% ,6 4 6 %的受调查者近两年内没有接受健康体检。当身体不适时 ,34 2 %的居民选择自我医疗。在 35岁以上的妇女中 ,5 8 3%没有接受乳房检查 ,6 3 9%没有接受宫颈涂片检查。提示我们社区居民潜在的卫生服务需求市场有待开发