宫内生长迟缓胎儿脐血IGF-Ⅰ及IGFBP-3水平观察

目的:探讨新生儿脐血中胰岛素样生长因子-Ⅰ(IGF-Ⅰ)和胰岛素样生长因子结合蛋白-3(IGFBP-3)水平与胎儿宫内生长发育的关系.方法:采用放射免疫分析,分别对出生时体重正常新生儿与宫内生长迟缓的低体重新生儿脐血的IGF-Ⅰ及IGFBP-3水平进行检测.结果:宫内发育迟缓组新生儿脐血的IGF-Ⅰ及IGFBP-3的含量明显低于正常对照组(P＜0.01),有显著差异性.结论::IGF-Ⅰ和IGFBP-3与胎儿宫内生长发育密切相关,对胎儿的宫内生长发育起着重要的调节作用.     

男性CHD患者血清T和LP（a）测定的临床价值

目的：探讨血清睾酮（T）和脂蛋白（a）[Lipoprotein（a）,LP（a）]测定对男性冠心病患者的诊疗价值。方法：对102例疑似冠心病（CHD）的男性患者进行冠状动脉造影检查,根据造影结果分为（CHD）病组和非（CHD）组,分别采用放射免疫分析和免疫透射比浊法进行血清T和LP（a）的测定,并对结果进行分析。结果：男性CHD患者血清T水平明显低于非CHD组（P〈0.01）,而血清LP（a）水平则显著高于非CHD组,两者之间呈负相关。结论：检测血清T和LP（a）对男性CHD患者的辅助诊断、疗效观察和预后判断具有重要价值。     

重点理工科高校大一新生焦虑状况及相关因素的研究

目的探讨重点理工科学校大一新生焦虑状况及相关因素,为进行针对性的干预提供依据。方法以广东某重点理工高校2007级的大一学生为研究对象,采用自行设计一般项目调查表、状态-特质焦虑量表（STAI）、匹兹堡睡眠质量指数量表（PSQI）、应付方式问卷进行测评。结果测查学生不论男女状态焦虑、特质焦虑分均显著高于地方常模;19.29%的学生睡眠质量较差;高状态焦虑组和高特质焦虑组解决问题、求助应付因子分均显著低于低状态焦虑组和低特质焦虑组,而自责、幻想、退避应付因子分则显著高于低状态焦虑组和低特质焦虑组;多元逐步回归分析说明,学生如常采用解决问题应付方式,在一定程度上可避免焦虑的产生,而睡眠质量差、采用自责应对方式则可加重焦虑水平。结论采取有效的认知应对策略其意义是使学生改变应激源,以使其变得威胁较小或变成挑战,把情绪基调训练成兴奋、期待和征服。     

全膝关节置换后不放置引流的相关管理策略

背景:近年来,随着全膝关节置换技术提升、手术时间缩短、术中及术后止血药的应用,其总失血量不断减少,术后引流量也逐渐减少,术后快速康复理论应运而生,引流管放置也再次成为骨科医生所热议的问题,越来越多的医生认为可以常规取消放置引流,引流似乎已可有可无。目的:旨在复习既往文献,对全膝关节置换后引流放置的相关问题归纳总结,提供围术期引流管理的相关经验。方法:检索中国知网、万方、维普、中国生物医学文献数据库等中文数据库,以及PubMed、The Cochrane Library、WebofScience、EMBASE、OVID等外文数据库,以"引流,膝关节置换术"及"totalknee arthroplasty,drainage"为检索词,检索自建库始至2020年2月为止所有与主题相关的文献,总结归纳全膝关节置换后不放置引流管围术期各方面需综合考虑的要素。结果与结论:①引流管的放置问题关乎全膝关节置换手术的最终结果,与术后出血、血肿、血栓、感染等并发症的发生关系密切;②目前全膝关节置换术后不推荐常规放置引流,但不意味着不需要管理,其实际上对临床医护在围术期各方面的管理提出更高的要求,包括但不限于术前适应证选择、手术操作、止血带使用、术中及术后药物使用、物理疗法的使用等。     

Rosiglitazone, an agonist of peroxisome proliferator-activated receptor γ, reduces pulmonary inflammatory response in a rat model of endotoxemia

Objective: The effect of rosiglitazone, a potent peroxisome proliferator-activated receptor γ (PPAR-γ) agonist, on pulmonary inflammation in endotoxemia was investigated. Materials and methods: Male Wistar rats were given either lipopolysaccharide (LPS, 6 mg/kg i.v.) or saline, pretreated with rosiglitazone (0.3 mg/kg i.v.) or its vehicle (dimethyl sulphoxide) 30 min before LPS. The selective PPAR-γ antagonist GW9662 (0.3 mg/kg i.v.) was given 20 min before rosiglitazone. Wet/dry weight (W/D) ratio, myeloperoxidase (MPO) activity, malondialdehyde (MDA) as well as TNF-α and CINC-1 concentrations were measured in lung tissues 4 h after LPS injection. Expression of ICAM-1, NF-κB p65 and PPAR-γ were also determined by immunohistochemistry or Western blot analysis. Results: Rosiglitazone pretreatment significantly attenuated the increases in W/D ratio, MPO activity and MDA levels, and reduced pulmonary overproduction of TNF-α and CINC-1 as well as expression of ICAM-1 following endotoxemia. Rosiglitazone also inhibited the nuclear localization of NF-κB and up-regulated the expression of PPAR-γ protein. The specific PPAR-γ antagonist GW9662 abolished the effect of rosiglitazone. Conclusion: These findings suggest that PPAR-γ agonists might be used as therapeutic agents in the therapy of inflammatory lung injury related to endotoxemia. Received 8 January 2005; returned for revision 6 July 2005; returned for final revision 20 July 2005; accepted by M. Katori 31 July 2005     

PTEN基因对人肝癌细胞系HHCC作用的研究

目的：研究PTEN基因对人肝癌细胞系HHCC细胞恶性表型及凋亡的影响。方法：将PTEN真核表达载体pBabe-PuroPTEN及空质粒pBabe-Puro，通过脂质体介导的基因转染方法，转入该基因表达缺失的HHCC细胞系中，嘌呤霉素筛选获得稳定表达PTEN基因的转染细胞。通过平板克隆形成试验、DNA凝胶电泳、相差显微镜和电镜，观察PTEN基因对人肝癌细胞系HHCC恶性表型及凋亡的影响。结果：转染后的细胞，经原位杂交、免疫组织化学检测证实，有PTEN mRNA及其蛋白的表达；平板克隆形成试验证实，转染PTEN基因后，细胞形成克隆的能力降低；转染PTEN的细胞在相差显微镜和电镜下可出现典型的凋亡形态学改变；DNA琼脂糖凝胶电泳呈现出明显的梯状条带。结论：外源性PTEN基因导入HHCC细胞后，可降低HHCC的细胞的恶性表型，并促进凋亡发生。     

Tacrolimus and mycophenolate mofetil after nonmyeloablative matched-sibling donor allogeneic stem-cell transplantations conditioned with fludarabine and low-dose total body irradiation.

We evaluated tacrolimus/mycophenolate mofetil (MMF) for graft-versus-host disease (GVHD) prophylaxis after a nonmyeloablative stem cell transplantation (NST) from a matched sibling donor (MSD). Thirty-two patients (median age, 57 years) with advanced hematologic malignancies, who were poor candidates for a conventional myeloablative transplantation, received fludarabine (30 mg/m(2), day -4 to day -2), total-body irradiation (TBI) (200 cGy, day 0), infusion of donor peripheral blood progenitor cells (day 0), oral tacrolimus 0.06 mg/kg twice daily (from day 3), and oral MMF at 15 mg/kg twice daily (days 0-+27). Tacrolimus was tapered from day +100 to day +180 in those patients with indolent malignancies (n = 25), and from day +35 to day +56 in those with aggressive tumors (n = 7). Regimen toxicities and myelosuppression were mild, allowing 75% of patients to have entirely outpatient transplantations. One patient (3%) experienced a nonfatal graft rejection. Rates of grades II-IV and III-IV acute GVHD were 15.6% and 3%, respectively. Acute GVHD was diagnosed at median day +78 (range, days +31-+84). Extensive chronic GVHD was observed in 10 of 24 evaluable patients (41.6%) at a median onset of day +198 (range, days +128-+277), either spontaneously (n = 5) or elicited after tumor progression (n = 5). Five patients experienced transplantation-related mortality (TRM) (15.6%) from either acute GVHD-related multiorgan failure (MOF) (n = 3) or infectious complications (n = 2). At median follow-up of 19 months (range, 2-41 months), the overall survival, progression-free survival, and disease-free survival rates are 62.5%, 50%, and 40%, respectively. In conclusion, the use of tacrolimus/MMF after MSD NST is associated with encouraging rates of GVHD control.     

Hypoxia can contribute to the induction of the Epstein-Barr virus (EBV) lytic cycle.

BACKGROUND: Like other herpes viruses, latent Epstein-Barr virus (EBV) infection can be reactivated to lytic replication. Reactivation can be achieved by treatment with various reagents, including tetradecanoyl phorbol acetate (TPA) and Ca2+ ionophores. Relatively little is known about the physiological factors related to reactivation of EBV. Previous studies have demonstrated that G0/G1 cell cycle arrest is associated with EBV activation, and that hypoxic conditions can induce cell cycle arrest. In the present study we investigated the effect of hypoxia on reactivation of EBV. OBJECTIVE AND METHODS: Hypoxic culture conditions were established and the expression of Zta protein and the number of EBV DNA copies were measured in B95-8 cells maintained under these conditions. RESULTS: Hypoxia treatment not only increased the expression of the EBV immediate-early protein Zta (which mediates the switch between the latent and lytic form of infection), but also increased the number of EBV DNA copies in B95-8 cells. CONCLUSIONS: EBV in latent infection can be activated to lytic infection by hypoxia treatment.     

High-level expression of EphA2 receptor tyrosine kinase in prostatic intraepithelial neoplasia

EphA2 is a transmembrane receptor tyrosine kinase that is overexpressed in many carcinomas. Specific targeting of EphA2 with monoclonal antibodies is sufficient to inhibit the growth, migration and invasiveness of aggressive cancers in animal models. Using immunohistochemical analyses, we measured the expression of EphA2 in prostatic adenocarcinoma, high-grade prostatic intraepithelial neoplasia, and adjacent benign prostate tissue from ninety-three radical prostatectomy specimens. These results were related to multiple clinical and pathologicalcharacteristics. The fraction of cells staining positively with EphA2 in benign prostatic epithelium (mean, 12%) was significantly lower than that in high-grade prostatic intraepithelial neoplasia (mean, 67%, P < 0.001) and prostatic adenocarcinoma (mean, 85%, P < 0.001). Moreover, the intensity of EphA2 immunoreactivity in prostatic adenocarcinoma was significantly higher than in benign prostatic tissue (P < 0.001) or high-grade prostatic intraepithelial neoplasia (P < 0.001). Benign prostatic epithelium showed weak or no immunoreactivity for EphA2 in all cases examined. Whereas EphA2 immunoreactivity related to neoplastic transformation, it did not correlate with other clinical and pathological parameters examined. Our data suggest that EphA2 levels increase as prostatic epithelial cells progress toward a more aggressive phenotype. Progressively higher levels of EphA2 in high-grade prostatic intraepithelial neoplasia and prostatic carcinoma are consistent with recent evidence that EphA2 functions as a powerful oncogene. Moreover, the presence of high levels of EphA2 in these cells suggests opportunities for prostate cancer prevention and treatment.     

人工晶体植入前后视觉诱发电位的临床分析

目的:(1)探讨白内障囊外摘除术前术后及人工晶体植入术后视觉诱发电位幅值及潜时变化特征;(2)探讨白内障囊外摘除术术前P—VEP,F—VEP,F—ERG联合预测的必要性。方法:采用重庆泰克医电仪器公司产TEC—100C视觉电生理仪。对白内障患者实行分组测量。术前均测F—ERG,P—VEP,部分病例增测F—VEP。结果:114例术前F—ERG正常,P—VEP测量其P_(100)波幅值潜时均异常:P_(100)波幅值降低,潜时延长,术后及人工晶体植入后幅值,潜时都有所改善,而并发性白内障,代谢性白内障变化不明显。结论:据视觉诱发电位的变化,老年性白内障组,外伤性白内障组术后应及时装入人工晶体,并发性白内障组,代谢性白内障组装入人工晶体临床意义不大。术前联合测试(VEP ERG)对视力预后、以及眼底病变的定量诊断意义重大。