Action of remantadine on fusion of the lipid envelope of influenza a virus with plasma and internal membranes in lymphoblastoid cells

Department of Biomembranes, Research Center for Development and Introduction of Modern Methods of Molecular Diagnosis, Ministry of Health of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR I. P. Ashmarin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 109, No. 5, pp. 483–485, May, 1990.     

Effect of interleukin 1 on adrenal function in mice

Laboratory of Regulation of Immunopoiesis, Institute of Clinical Immunology, Siberian Branch, Academy of Medical Sciences of the USSR, Novosibirsk. (Presented by Academician of the Academy of Medical Sciences of the USSR V. P. Kaznacheev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 105, No. 3, pp. 311–313, March, 1988.     

Effect of single total irradiation on the reproductive system and vitamin content in rat progeny

Reduction in the epididymis weight and in the spermatozoid concentration together with marked morphological changes in the seminiferous tubules were observed in the progeny of irradiated rats. Vitamin E content in the testes increased 2.8-fold, while that of vitamin B₂ decreased 1.6-fold. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 12, pp. 652–654, December, 1998.     

Development of sandwich radioimmunometric assay for serum c-erbB-2 oncogene product and its significance in diagnosing breast carcinoma]

A sandwich radioimmunometric assay using monoclonal antibodies 6G10 and SV2-61 gamma directed to the extracellular domain of the c-erbB-2 oncogene product was developed and the antigen levels in sera from normal donors and patients with breast carcinoma were determined. The antigen levels in normal donors were uniformly low (9.52 +/- 0.91 ng/ml) and 3.0% (2/66 cases) slightly exceeded the cutoff value (11.4 ng/ml). In patients with breast carcinoma, serum c-erbB-2 protein levels increased and the positivity was as high as 45.7% (16/35 cases) in recurrent cases. Determination of c-erbB-2 protein may be a useful marker for serological diagnosis of breast carcinoma.     

Hepatitis B virus propagated in a rat hepatoma cell line is infectious in a primate model

The human hepatitis B virus (HBV) produced by a rat hepatoma cell line through transfection with HBV DNA is infectious in the human primate model--chimpanzee. Since hepadnaviruses are known to have an extremely narrow host range, our results support the idea that the host species barrier of HBV infection resides on the penetration/adsorption step rather than any postpenetration intracellular event during the virus life cycle.     

葡萄糖和胆固醇对人肝细胞ANGPTL3基因和蛋白质表达的影响

目的 观察葡萄糖和胆固醇对人肝细胞株(L02)血管生成素样蛋白3(angiopoietin-like pro-tein3,ANGPTL3)表达的影响,探讨其与糖尿病和高胆固醇血症的关系.方法 以L02细胞为研究对象,将其分为葡萄糖组(G)和胆固醇组(CH).分别在葡萄糖浓度为5.6 mmol/L(G1)、7.0 mmol/L(G2)、11.1 mmol/L(G3)、28.0 mmol/L(G4)、33.0 mmol/L(G5)的培养液和含胆固醇10 μmol/L、25 μmol/L、50 μmol/L、100 μmol/L的培养液中培养.采用RT-PCR方法检测各组细胞ANGPTL3 mRNA表达量,用Western印迹方法检测其蛋白质表达水平.结果 G3～G5组葡萄糖可剂量依赖性地增加ANGPTL3的mRNA表达(P<0.05);在蛋白质表达水平,G2～G5组与G1组比较差异均有统计学意义(P<0.05);G1～G5组各组间两两比较,除G4组与G5组呈现表达量虽升高,但差异无统计学意义(P>0.05)外,其它各组间差异均有统计学意义(P<0.05).4种胆固醇浓度均可促进L02细胞ANGPTL3的mRNA和蛋白质表达(P<0.05).结论 在一定浓度范围内葡萄糖可剂量依赖性地促进ANGPTL3 mRNA和蛋白质表达;AN-GPTL3的mRNA和蛋白质表达量还受环境胆固醇浓度影响.ANGPTL3的表达可能与糖尿病、高胆固醇血症密切相关.     

HIV-1膜抗原部分糖基化位点改造对免疫原性及假病毒形成的影响

目的 研究HIV-1膜蛋白(Env)特定糖基化位点改造对Env免疫原性及功能性假病毒形成能力的影响.方法 采用环形诱变,DpnⅠ筛选的方法对Env进行定点突变,单周期感染试验检测功能性假病毒形成能力,免疫小鼠,利用假病毒中和试验与ELISPOT分别检测突变对中和抗体和T细胞分泌Env特异的IFN-γ的影响.结果 N197Q突变体使Env诱导中和抗体的能力提高而诱导T细胞分泌Env特异的IFN-γ的能力降低,并使Env不能形成功能性假病毒;G2突变体(N624Q,N637Q)诱导的中和抗体能更好地中和假病毒74-2,仉中和假病毒Wt的能力下降,对Env诱导T细胞分泌Env特异的IFN-γ的能力和功能性假病毒形成无明显影响.结论 特定糖基化位点的改造影响假病毒的形成及Env的免疫原性.     

Use of Transcranial Magnetic Stimulation with Measurement of Motor Evoked Potentials in the Acute Period of Hemispheric Ischemic Stroke

Functional derangements in the brain during the acute period of ischemic hemispheric stroke (IS) were assessed in terms of the severity of the motor neurological deficit in the acute period of IS and neurophysiological measures of motor evoked potentials (MEP) using transcranial magnetic stimulation (TCMS). A total of 52 patients (23 women, 29 men, mean age 58.5 ± 8.7 years) were studied. Patients were divided into two subgroups: group 1 consisted of 29 patients with good functional outcomes from the acute period; group 2 consisted of 23 patients with poor functional outcomes. The use of TCMS for recording MEP demonstrated increases in the latency of the M response both after stimulation of the projection of the motor area of the cortex of the lesioned hemisphere and after stimulation of the spinal cord. There were increases in the central motor conduction time (CMCT) in the lesioned hemisphere of the brain and a negative correlation was seen between the severity of the neurological defect and CMCT on the one hand (r = –0.65 to –0.78; p < 0.001) and, on the other, the latency of the M response in TCMS of the motor zone of the cortex on the side of the hemispheric stroke (r = –0.65 to –0.79; p < 0.001). The increases in the latency of the M response and CMCT have prognostic significance for early assessment of the outcome of IS.     

建立一种用重组蛋白检测抗口蹄疫病毒抗体的间接ELISA方法

目的研究以重组蛋白作为包被抗原检测口蹄疫病毒（FMDV）感染后的动物血清中特异性抗体的可能性,为建立一种非病毒颗粒的ELISA检测试剂盒提供实验依据。方法利用自行构建表达的O型口蹄疫病毒VP1表位肽重组蛋白（VP1epi）作为包被抗原,采用间接ELISA方法确定抗原的最佳工作浓度和包被方法,优化各项实验条件,并以FMDV感染后的豚鼠血清作为标准阳性血清确定ELISA方法的特异性和灵敏度。结果FMDV感染后的阳性豚鼠血清可以很好地识别VP1epi重组蛋白,用此蛋白包被检测抗FMDV抗体的灵敏度可达1∶3200,并证明所检测的抗体是FMDV特异性的。结论VP1epi重组蛋白可以替代FMDV颗粒用于建立检测抗FMDV抗体的ELISA试剂盒。     

恒河猴骨髓间充质干细胞分离培养及其生长特性

采用密度梯度离心和贴壁筛选方法分离纯化了恒河猴骨髓间充质干细胞(mesenchymal stem cells from bone marrow of Rhesus monkey,RhBMSCs);通过表面抗原检测和核型分析对细胞进行了初步鉴定;通过生长曲线的绘制和细胞凋亡的检测探讨了细胞的生长特点。本实验获得的RhBMSCs形态以梭型为主,核型正常,CD29表达率为99.2%,CD34、CD45、HLA-DR表达率均低于3.2%,RhBMSCs表型符合间充质干细胞的特点,且纯度较高,细胞生长旺盛,但倍增时间有随传代数的增加而逐渐延长的趋势。本实验探讨了RhBMSCs的体外分离、扩增培养及鉴定的方法,并对其生长特点进行了初步观察,这不仅有助于进一步了解与之近似的人的骨髓间充质干细胞的相关特性,而且为以猕猴为对象的BMSC定向分化和组织修复等动物体内实验打下了基础。