天然有机化合物质谱研究——Ⅺ.苯丙型苷快原子轰击质谱MNa~+的MIKES谱

研究了六种苯丙型苷与Na~+加合离子的快原子轰击质谱(FAB—MS)和质量分离离子动能谱(MIKES)。结果表明:在FAB谱中[M+Na]~+加合离子的丰度要比[M+H]~+离子高得多。由此可给出糖苷的分子量信息,[M+Na]~+离子的MIKES谱可给出糖基序列信息。     

九里香抗生育活性物质——月橘烯碱的仿生合成研究

月橘烯碱是从芸香科植物九里香根中分出的双吲哚生物碱。药理实验结果表明它具有抗着床和抗早孕的活性。本文报道其仿生合成:吲哚经3位醛基化、与丙酮缩合成化合物Ⅱ;将氮原子保护后进行wittig反应,脱保护基得到β-异戊二烯吲哚(Ⅴ);经分子间Diels-Alder加成反应得消旋月橘烯碱(Ⅵ)。     

三尖杉酯碱类似物的合成及其抗肿瘤活性

合成了新结构类型的三尖杉酯碱类似物C₁Z,C₂E,C₃E,C₄E,考察了侧链烯酯酸B₁,B₂,B₃,B₄形成时顺、反异构体的比例,发现顺式构型在酸催化下易转化成反式构型。C₁E对肿瘤细胞HL-60有明显的分化诱导作用及对白血病L₁₂₁₀有抑制作用。     

地塞米松治疗难治性支扩咯血45例临床分析

0引言支气管扩张感染并咯血一般治疗并不困难,有些病例用常规综合治疗未能控制者,称之为难治性支扩咯血,此时加用地塞米松可收到良好效果.现就我科自1995-02/2005-03收治的216例支扩咯血患者治疗情况报告如下.     

支气管败血鲍特氏菌致肾脓肿1例

1 病例报告患者,男,49岁,因头部撞地后意识障碍进行性加重6 h入唐都医院神经外科,临床症状: 呕吐频繁,躁动不安,深昏迷,呼吸困难,右侧瞳孔散大,对光反射消失,双侧巴氏征( ),双肺布满湿性罗音.CT示右额颞部硬膜外血肿、中线左移2.5 cm.急诊开颅清除血肿及气管切开.术后意识障碍渐好转,但第10日开始持续高热,呼吸道分泌物多,痰培养见支气管败血杆菌生长.多次尿检均未见明显异常.血常规: WBC 19.6×109/L,中性0.99,淋巴0.01,核左移.反复动脉血培养均为阴性.按肺部感染给予抗炎及雾化吸入等治疗,肺部感染得到控制,但仍发热.术后28 d发现患者左腹部一压痛包块,B超提示左肾体积增大,内有液性暗区.肾穿刺放出灰白色粘稠无臭味脓液约2500 mL,细菌培养为支气管败血杆菌.引流5 d后体温便恢复正常,后行左肾切开造瘘术并置造瘘管引流,2 mo后痊愈.     

LHRH类似物的设计和合成

为了寻找新的高效LHRH类似物,设计并合成了带有(D-Trp~6、desGly~(10))-LHRHEA,(D-Arg~6,desGly~(10))-LHRHEA变换5位残基的六个类似物。并应用体外黄体细胞培养法测定了它们的生理活性。     

牛黄解毒片的三维高效液相色谱法鉴定和指标成分的定量

用三维高效液相色谱(three dimensional HPLC)法对中成药牛黄解毒片进行了分析。结果表明该法用于牛黄解毒片中单味中药鉴定及指标成分(marker substance)含量测定均得到较满意结果。     

逍遥丸的三维高效液相色谱法鉴定和指标成分的定量

用三维高效液相色谱(three dimensional HPLC)法对中成药逍遥丸进行了分析。结果表明该法用于逍遥丸中单味中药鉴定及其指标成分(marker substances)含量测定均得到满意结果。     

Detection of HIV antigen and specific antibodies to HIV core and envelope proteins in sera of patients with HIV infection

The sera of well-characterized populations were examined for three markers of human immunodeficiency virus (HIV) infection; HIV antigen (HIV Ag), and antibodies to HIV envelope (gp41) and core (p24) proteins. Of 563 serum samples tested, 251 were from HIV-infected patients diagnosed as having AIDS manifested by opportunistic infections (AIDS-OI), AIDS-associated Kaposi's sarcoma (AIDS-KS), or AIDS-related complex (ARC). One hundred seventy-six specimens tested were from asymptomatic high-risk individuals, and 136 were from heterosexual control subjects or patients with non-AIDS-related disease. None of the 136 control individuals tested had HIV Ag or HIV antibodies to either p24 or gp41. Of the 427 HIV-seropositive individuals, 99% to 100% were positive for gp41 antibodies to HIV. In contrast, the seroprevalence of p24 antibodies to HIV varied from 23% to 83% and appeared to be inversely associated with the severity of the patients' clinical symptoms. When specimens were analyzed for the presence of HIV Ag, in seropositive individuals the prevalence rate for this marker was lowest (1.4%) in asymptomatic individuals and highest (50%) in the AIDS-OI diagnosed group. Also, 240 cases with AIDS-KS, AIDS-OI, and ARC and the group of asymptomatic high-risk individuals were analyzed for T helper/T lymphocytes (T4) cell number and T4/T8 ratio; only one (2.0%) HIV Ag-positive case showed a T4 cell number greater than 400 and a normal T4/T8 ratio. These studies appear to demonstrate a direct correlation between the presence of HIV Ag and the severity of clinical complications of HIV infection.