大鼠脑神经干细胞神经巢蛋白表达的流式细胞术检测

检测胎鼠及成年大鼠脑内神经干细胞特异性抗原神经巢蛋白的表达,判明啮齿类哺乳动物脑内神经干细胞存在的可能性及相应的部位。方法：显微手术下采取胎鼠及成年大鼠大脑与皮质下脑组织,间接荧光法标记ｎｅｓｔｉｎ,以流式细胞术检测。结果胎鼠脑内普遍存在ｎｅｓｔｉｎ阳性细胞,成年大鼠脑室旁组织中也存在ｎｅｓｔｉｎ阳性细胞。     

嗓音频谱分析中／a／,／i／音采样的比较

应用486微型计算机和北京邮电大学通用信号谱分析系统分别对30例青年女性喉病患者和36例健康对照组的/a/、/i/音嗓音信号进行频谱分析,比较其相对信噪比,结论是在频谱分析中用/a/音采样较/i/音更易检出病态嗓音.     

A longitudinal,functional study of peripheral nerve recovery in the mouse

Objectives: To verify the validity of the recently described sciatic functional index for mice to monitor neuronal functional recovery over time using a blinded, randomized, and controlled evaluation. Study Design: Surgery was performed on the left sciatic nerves of 62 C57/BL mice after randomly assigning them to one of four surgical groups: sham surgery, sciatic nerve crush, nerve transection without repair, and nerve transection followed by epineurial suture repair. Sciatic functional indices were measured before surgery and then after surgery at 10-day intervals for 90 days, using a previously described formula. Results: Sham surgery did not affect nerve function when compared with preoperative values (P > .24). Crush surgery produced a reversible nerve injury that fully recovered after 20 days. Nerve transection without repair resulted in complete functional disability without recovery of function during the 90-day follow-up interval. When transected nerves were repaired, complete functional disability was noted at day 10, with subsequent functional recovery to 26% of function at day 30. This level of recovery persisted until the 60th postoperative day when muscle contractures resulted in progressive worsening of the index. There were statistically significant differences between the sciatic functional indices of each of the groups (P < .05). Conclusions: The previously described sciatic functional index for mice is an accurate indicator of the level of sciatic neuronal function during recovery. This index represents a method of evaluating neuronal function that may provide a better reflection of the recovery parameters that are important in clinical situations. The sciatic functional index will allow for study of sciatic nerve functional recovery in genetically engineered transgenic mice.     

The effect of Rhodiola capsules on oxygen consumption of myocardium and coronary artery blood flow in dogs]

It has been proved that Rhodiola Capsules can obviously decrease the oxygen consumption of myocardium and oxygen consumption index in anesthetic dogs, decrease the coronary artery resistance, but have no marked effect on the coronary artery blood flow. The Capsules also have the function of decreasing blood presure and slowing heart rate.     

钢基Cu-5%Al激光熔覆层颗粒组织分布的定量分析

熔覆层中的颗粒相分布不仅影响材料性能，同时也是研究熔覆过程各种反应机制的主要依据。本文利用图像分析技术，定量分析了Ｑ２３５钢表面Ｃｕ５％Ａｌ激光熔覆层的富铁相颗粒分布情况。发现熔覆过程中在熔池中存在熔体对流环，熔池中的对流带动颗粒运动，是决定熔覆层颗粒分布的主要因素。     

养血清脑颗粒治疗老年期痴呆的临床疗效观察

目的：观察养血清脑颗粒对老年期痴呆的疗效。方法：治疗组老年期痴呆患者36例均应用养血清脑颗粒。详录治疗前后实验室检查数据。健康老年人34例为对照组，以其体检时所得相关指标与治疗组比较分析。结果：有效率85％，S-TC明显下降，P-SOD明显升高，P-LPO，Ht，Va降低、EET缩短。结论：养血清脑颗粒具有降脂、抗自由基损伤。改善异常血液流变，增加脑血流量等功用。对老年期痴呆防治有较好效果。     

Foreign metallothionein-I expression by transient transfection in MT-I and MT-II null astrocytes confers increased protection against acute methylmercury cytotoxicity

The mechanisms associated with metallothionein (MT) gene regulation are complex and poorly understood. Only a modest increase in brain MT expression levels is attained by exposure to metals, MT gene transfection, and MT gene knock-in techniques. Accordingly, in the present study, MT null astrocytes isolated from transgenic mice deficient in MT-I and MT-II genes were introduced as a zero background model of MT expression. MT protein levels were determined by western blot analysis. MT proteins in MT-I and MT-II null astrocytes were undetectable. Transient MT-I gene transfection increased the levels of foreign MT expression in MT-I and MT-II null astrocytes by 2.3-fold above basal levels in wild-type astrocytes. Intracellular Na(2)51CrO(4) efflux and D-[2,3-3H]aspartate uptake were studied as indices of acute methylmercury (MeHg) (5 microM) cytotoxicity. In MT-I and MT-II knockout astrocytes MeHg led to significant (p<0.01) increase in Na(2)51CrO(4) efflux and a significant (p<0.05) decrease in the initial rate (1 min) of D-[2, 3-3H]aspartate uptake compared to MT-I and MT-II knockout controls. Transfection of the MT-I gene in MT-I and MT-II null mice significantly (p<0.01) decreased the effect of MeHg on Na(2)51CrO(4) efflux in MT null, as well as wild-type astrocytes. MT-I gene transfection in MT-I and MT-II null astrocytes reversed the inhibitory effect of MeHg on D-[2,3-3H]aspartate uptake, such that initial rates of uptake in MT-I transfected cells in the presence and absence of MeHg (5 microM) were indistinguishable. These results demonstrate that: (1) astrocytes lacking MTs are more sensitive to MeHg than those with basal MT protein levels, (2) the MT-I gene can be overexpressed in MT-I and MT-II null astrocytes by transient MT-I gene transfection, and (3) that foreign MT expression endows astrocytes with increased resistance to MeHg.     

CA125单独及联合多种抗原标志物对卵巢癌的诊断价值

目的 :探讨多种肿瘤标志物对卵巢癌的诊断价值。方法 :应用化学发光法测定卵巢肿瘤、子宫内膜异位症和盆腔炎性包块的CA1 2 5、CA1 99、CA1 5 3、AFP、CEA阳性率。结果 :卵巢癌患者中 ,80 .6 0 %CA1 2 5阳性 ,92 .5 3%联合检测阳性 ,阳性率明显高于良性肿瘤、子宫内膜异位症、盆腔炎性包块患者 (P <0 .0 1 ) ;CA1 2 5与卵巢癌的临床分期及病理类型无关 ,但Ⅰ期卵巢癌患者的血清CA1 2 5值明显低于Ⅱ～Ⅳ期患者 (P <0 .0 1 )。结论 :CA1 2 5对卵巢癌的诊断有一定的价值 ,联合监测能提高卵巢癌的诊断率 ,尤其对早期卵巢癌。     

综合治疗发育性弱视243例

目的:探讨各种类型发育性弱视治疗的疗效。方法:对243例发育性弱视患者进行包括验光配镜、遮盖治疗、红光闪烁治疗、后像疗法、精细目力训练等的综合治疗。结果:基本治愈率为56.4%,有效率为92.4%,其中轻度弱视的治愈率为81.6%,中度为41.1%,重度为24.3%;屈光不正性弱视的治愈率为62.9%,斜视性弱视为49.0%,屈光参差性弱视为43.1%,形觉剥夺性弱视为18.8%;中心注视的治愈率为65.3%,旁中心注视为20.7%;年龄≤6岁的治愈率为82.7%,年龄在7~9岁间的为49.4%,年龄≥10岁的为19.6%。结论:弱视的治疗效果与弱视程度、类型、注视性质、初诊年龄等密切相关,早期发现,早期治疗效果好。综合疗法的疗效优于单一治疗方法。     

Psychometric evaluation of the Taiwan version of the Disability of the Arm, Shoulder, and Hand (DASH) questionnaire.

BACKGROUND AND PURPOSE: To adapt the Taiwan version of the Disability of the Arm, Shoulder, and Hand (DASH) questionnaire and evaluate its psychometric properties. METHODS: The DASH questionnaire was adapted through the process of translation, back-translation, and expert review. Eighty two subjects with upper extremity disorders were recruited in a medical center and 46 of these patients could be followed up to assess retest reliability in less than 10 days. Cronbach alpha and intraclass correlation coefficient were used to evaluate the internal consistency and test-retest reliability. Principal axis factor analysis was performed to assess the factor-construct validity, while concurrent validity was tested with the Medical Outcomes Study Short Form-36 (SF-36) Taiwan version questionnaire. RESULTS: The internal consistency of the Taiwan version of the DASH questionnaire was high (Cronbach alpha = 0.96) and the test-retest reliability was satisfactory (intraclass correlation coefficient = 0.9). Principal axis factor analysis confirmed the 1-factor model. The Pearson correlation coefficients of the DASH questionnaire to the SF-36 showed a correlation with physical component summary scores rather than mental component summary scores. Bodily pain, physical function and role-physical scores among the SF-36 subscales were most significantly correlated with DASH disability/symptom scores. CONCLUSION: The Taiwan version of the DASH questionnaire is a valid and reliable measure of health status for patients with upper-extremity disorders.