宫颈开口监测仪器的研制

介绍一种新研制成功的宫颈开口监测仪器,该仪器是基于单片机系统的、全中文操作界面的、便携式仪器.从工程上解决了对产妇宫颈开口那样一种特殊环境下,特殊生理位置的实时监测问题.     

近距离放射治疗仪和适形调强放疗机的探讨

放疗仪器是利用电离辐射对肿瘤进行治疗的装置;近距离放疗仪器是指将封装好的放射源经人体腔道放在肿瘤体附近或表面,或是将细针管插植于肿瘤体内导入射线源实施照射的放疗技术.通过改变射束剖面强度分布,达到形状适形和剂量适形,叫做调强适形放疗技术.     

耳穴疗法治疗老人失眠症之临床研究

目的:尝试利用科学性之方法探讨耳穴磁疗对老人失眠症之效果.方法:120位60岁或以上之老年患者,随机分配入三组.第一组及第二组为对照组,分别贴有灯芯或王不留行籽(不加压);第三组为实验组,贴有每粒含66高斯之磁珠(不加压).为针对老年患者常见之脏腑功能衰弱而致失眠,各人均选取相同之穴位:神门、心、肾、肝、脾、皮质下及枕,疗程共3周.结果:疗程完毕后,使用磁珠作耳疗之实验组于治疗前后的睡眠效果指数有明显之改进(P＜0.05),显效17%,有效为65%,总有效率为82%.此外,利用多元回归之反向淘汱法分析女性参加者之数据,发现利用磁珠作耳疗及参加者年龄对预测睡眠效果指数达显著水准(F3,106=9.04,P＜0.001,R2=0.20).结论:耳穴磁疗能有效地改善老人失眠症.     

Epitopes of human brain acetylcholinesterase

The main purpose of the present work was to identify B-cell epitopes on human brain acetylcholinesterase (AChE) by the synthetic peptide approach. Five hundred and seventy-four decapeptides comprising amino acids No. n to n+9 (where n denotes the residue number of the 583 amino acids in the primary structure of human brain AChE and is an integer in the range 1-574) were synthesized, using the multipin combinatorial chemical synthesis technique, and biotinylated. Epitopes of human brain AChE were detected by enzyme-linked immunosorbent assay (ELISA) and compared with the predicted epitopes of human AChE by 'Goldkey' software. Among 574 synthetic decapeptides, 47 decapeptides at 11 antigenic regions showed immunoreactivity with mouse anti-human brain AChE polyclonal antibodies. The minimum sequence of epitope was defined at every antigenic region explored. The locations and sequences of the former ten continuous epitopes at the 11 antigenic regions of the human brain AChE had been identified as follows: TPVLVWIY (112-119), RTVLVSMNY (143-151), LLDQRLALQW (173-182), RRATQLAH (246-253), VFRFSFVPV (294 approximately 302), KDEGSYFLVY (332-341), RVYA (424-427), LMRY (476-479), KAPQWPPY (496-503), GLRAQACAFW (523-532). The rate of hits of the predicted epitopes from the software came out at 33%. In our work, the epitopes of human AChE have been mapped by purified polyclonal antibody at eleven distinct sites in the primary structure.     

CoQ10 fails to protect brain against focal and global ischemia in rats

OBJECTIVE: Release of oxygen free radicals occurs following cerebral ischemia. Studies show that oxygen free radicals mediate ischemic brain injury. CoQ10 is a potent free radical scavenger and may offset brain injury associated with reperfusion. We tested exogeneous CoQ10 as a neuroprotectant in rats following both global and focal ischemic insults. METHODS: Rats were subjected to either 4-vessel occlusion ischemia (4-VO, 10 min occlusion, 7-day survival) or middle cerebral artery occlusion (MCAO, 120 min-occlusion, 22.5 h survival). Regional cerebral blood flows (rCBF) and physiological variables such as blood pressure, pO2, pCO2, plasma glucose and hematocrit were monitored and measured in focal ischemia. The animals were randomized to receive treatments of either phosphate buffered saline (PBS) vehicle or CoQ10 following global or focal ischemia. Injection times were at the end of ischemia and 3 h later for both models of ischemia. Histological outcomes are expressed as a percentage of hippocampal CA(1) cell injury in global ischemia or percentage of cortical infarct over that of non-ischemic hemisphere in focal ischemia. RESULTS: In global ischemia, animals treated with PBS vehicle and CoQ10 had 86+/-5% (n=8) and 83+/-10% (n=8), respectively, of hippocampal CA(1) cell injury (P>0.05). The percentage of infarct volumes in animals following focal ischemia were 23+/-9% (control, n=10) and 25+/-9% (CoQ10, n=10). There were no temperature or physiological differences between the two treatment groups. CONCLUSION: Acute treatment with CoQ10 via intraperitoneal injection does not prevent neuronal injuries following global and focal ischemia.     

The influence of human intervertebral disc tissue on the metabolism of osteoblast-like cells

Extensive studies have been performed to evaluate different factors that may affect on spinal interbody fusion, but the role of intervertebral disc tissue in the fusion process remains unclear. To study the influence of intervertebral disc tissue on osteoblast metabolism, we harvested disc tissue from patients who had undergone spinal surgery. The nucleus pulposus and annulus fibrosus were separately co-cultured with osteoblast-like cells SaOS-2 by means of culture inserts or organ culture. We assayed alkaline phosphoatase activity, 3H-thymidine incorporation into the DNA, and production of collagen type I, IL-1beta, IL-6, IL-10, and TNF-alpha. Exposure of the nucleus pulposus (NP) to osteoblast-like cells revealed stimulation of alkaline phosphatase production, 3H-thymidine incorporation and collagen type I production. Exposure of the annulus fibrosus (AF) stimulated 3H-thymidine incorporation and collagen type I production, but did not affect ALP activity. IL-6 was detected after application of NP and AF. Interleukin IL-10, IL-1beta and TNF-alpha were all below detection levels after application of disc tissue. Our findings show that frozen disc tissue stimulates the metabolism of osteoblast-like cells in vitro.     

共轭亚油酸对肥胖大鼠PPARγ基因表达及血清瘦素水平的影响

目的　研究不同剂量共轭亚油酸 (CLA)对饮食诱导肥胖大鼠PPARγ基因、瘦素、血糖、血脂的影响。方法　选用雄性Wistar大鼠 ,随机分为对照组、高脂组、高脂 +CLA组 (每 10 0g饲料含CLA分别为 0 75g、1 5 0g、3 0 0g) ,于第 12周末处死动物 ,计算脂 体比 ,测定大鼠血糖、血脂及瘦素水平 ,并应用RT PCR的方法检测大鼠白色脂肪组织过氧化物酶体增殖物激活受体γ(PPARγ)的表达水平。结果　CLA可降低肥胖大鼠血糖、甘油三酯 (TG)、总胆固醇 (TC)及瘦素水平 ,增加脂肪组织PPARγmRNA的表达水平。结论　CLA可降低肥胖大鼠血糖、血脂 ,并可通过激活PPARγ下调瘦素水平 ,有改善肥胖大鼠的瘦素抵抗作用。     

应用飞行时间质谱仪快速鉴定细菌的初步研究

目的　研究基质辅助激光解析电离飞行时间质谱对细菌快速鉴定的影响因素。方法　应用 8种细菌 ,比较了不同培养基 (哥伦比亚血琼脂、伊红美兰琼脂、普通营养琼脂 )及培养时间对细菌质谱图的影响。结果　不同的培养基及培养时间会对细菌质谱图产生影响。结论　选择合适的培养基及培养时间可以提高细菌检测的准确率     

所有权与经营权分离是城市公立医院改革和发展的途径

传统卫生管理体制承袭了计划经济的所有特征,价格扭曲的宏观政策环境、资源的集中计划配置体制与公立医院在微观上的缺乏经营自主权三者并存.这种制度安排越来越不适应市场经济条件下卫生改革和发展的需要,而卫生经济体制改革始自微观经营机制,核心是两权分离和放权让利,这是自然的,同时也是正确的改革方向.