全文获取类型
收费全文 | 1078篇 |
免费 | 75篇 |
国内免费 | 28篇 |
专业分类
儿科学 | 28篇 |
妇产科学 | 153篇 |
基础医学 | 128篇 |
口腔科学 | 24篇 |
临床医学 | 88篇 |
内科学 | 134篇 |
皮肤病学 | 15篇 |
神经病学 | 31篇 |
特种医学 | 158篇 |
外科学 | 56篇 |
综合类 | 125篇 |
预防医学 | 32篇 |
眼科学 | 28篇 |
药学 | 139篇 |
1篇 | |
肿瘤学 | 41篇 |
出版年
2023年 | 6篇 |
2021年 | 9篇 |
2020年 | 8篇 |
2019年 | 4篇 |
2018年 | 9篇 |
2017年 | 11篇 |
2016年 | 11篇 |
2015年 | 11篇 |
2014年 | 16篇 |
2013年 | 27篇 |
2012年 | 33篇 |
2011年 | 29篇 |
2010年 | 27篇 |
2009年 | 42篇 |
2008年 | 40篇 |
2007年 | 59篇 |
2006年 | 48篇 |
2005年 | 27篇 |
2004年 | 39篇 |
2003年 | 25篇 |
2002年 | 23篇 |
2001年 | 22篇 |
2000年 | 29篇 |
1999年 | 27篇 |
1998年 | 41篇 |
1997年 | 43篇 |
1996年 | 50篇 |
1995年 | 31篇 |
1994年 | 25篇 |
1993年 | 23篇 |
1992年 | 22篇 |
1991年 | 25篇 |
1990年 | 21篇 |
1989年 | 46篇 |
1988年 | 32篇 |
1987年 | 26篇 |
1986年 | 36篇 |
1985年 | 22篇 |
1984年 | 14篇 |
1983年 | 12篇 |
1982年 | 22篇 |
1981年 | 13篇 |
1980年 | 18篇 |
1979年 | 6篇 |
1978年 | 7篇 |
1977年 | 10篇 |
1976年 | 12篇 |
1975年 | 13篇 |
1974年 | 4篇 |
1966年 | 3篇 |
排序方式: 共有1181条查询结果,搜索用时 0 毫秒
81.
Obstetric and nonmalignant gynecologic bleeding: treatment with angiographic embolization 总被引:4,自引:0,他引:4
Eight patients (seven post partum, one post abortion) with massive pelvic hemorrhage related to pregnancy and one patient with uncontrollable bleeding following a cervical biopsy underwent angiography to facilitate the identification and treatment of bleeding sites. In all nine patients pelvic hemorrhage was successfully controlled with embolization under angiographic guidance. Angiographic embolization allowed preservation of the uterus in six patients referred prior to hysterectomy, and one patient subsequently became pregnant. When conservative measures and minor surgical repairs have failed, embolization should be the next step in the treatment of postpartum hemorrhage to avoid major surgery in an unstable patient and to maintain reproductive function. 相似文献
82.
Liu SC; Palek J; Yi SJ; Nichols PE; Derick LH; Chiou SS; Amato D; Corbett JD; Cho MR; Golan DE 《Blood》1995,86(1):349-358
Southeast Asian ovalocytosis (SAO) is an asymptomatic trait characterized by rigid, poorly deformable red cells that resist invasion by several strains of malaria parasites. The underlying molecular genetic defect involves simple heterozygous state for a mutant band 3 protein, which contains a deletion of amino acids 400 through 408, linked with a Lys 56-to-Glu substitution (band 3-Memphis polymorphism). To elucidate the contribution of the mutant SAO band 3 protein to increased SAO red blood cell (RBC) rigidity, we examined the participation of the mutant SAO band 3 protein in increased band 3 attachment to the skeleton and band 3 oligomerization. We found first that SAO RBC skeletons retained more band 3 than normal cells and that this increased retention preferentially involved the mutant SAO band 3 protein. Second, SAO RBCs contained a higher percentage of band 3 oligomer-ankyrin complexes than normal cells, and these oligomers were preferentially enriched by the mutant SAO protein. At the ultrastructural level, the increased oligomer formation of SAO RBCs was reflected by stacking of band 3-containing intramembrane particles (IMP) into longitudinal strands. The IMP stacking was not reversed by treating SAO RBCs in alkaline pH (pH 11), which is known to weaken ankyrin-band 3 interactions, or by removing the cytoplasmic domain of band 3 from SAO membranes with trypsin. Finally, we found that band 3 protein in intact SAO RBCs exhibited a markedly decreased rotational mobility, presumably reflecting the increased oligomerization and the membrane skeletal association of the SAO band 3 protein. We propose that the mutant SAO band 3 has an increased propensity to form oligomers, which appear as longitudinal strands of IMP and exhibit increased association with membrane skeleton. This band 3 oligomerization underlies the increase in membrane rigidity by precluding membrane skeletal extension, which is necessary for membrane deformation. 相似文献
83.
0 引言 人类免疫缺陷病毒 (human immunodeficiencyvirus,HIV) - 1编码的反式激活蛋白 TAT具有独特的跨膜运转方式 ,而且有转导速度快 ,效率高的特点 ,被称为蛋白转导结构域 (protein transduction domain,PTD) [1 ,2 ] .本研究用PCR扩增了慢性粒细胞白血病慢粒 bcr/ abl融合蛋白的基因片段 ,在其 5′端融合 PTD结构域的编码区后在大肠杆菌中进行了表达 .表达产物经纯化后 ,加入培养的 HL 6 0细胞 ,表达的蛋白可直接进入细胞内 .这一结果为用外源蛋白负载(L oading)免疫细胞提供了新的途径 .1 材料和方法1.1 DNA重组 人工合… 相似文献
84.
85.
86.
J W Witkin 《Neurobiology of aging》1986,7(4):259-263
Luteinizing hormone releasing hormone (LHRH) elements were demonstrated immunocytochemically in coronal sections of a portion of the left hypothalamus in twenty female rhesus macaques ranging in age from just over two years to over 21 years. This time period includes the juvenile preovulatory phase. The region studied included the retrochiasmatic region as far dorsally as the superior extent of the optic tract and as far posteriorly as the mammillary body. There was a range in total LHRH neurons per animal from 55 to 470. No trends in cell number as a function of age was observed. The range in cell number among the youngest animals (two year olds) was about the same as that in adult (6-15 year olds) and older (20+ year olds) animals. These results suggest that there is no age-related decline in the potential for manufacture of LHRH, at least into the pre-menopausal period, in the female rhesus macaque. 相似文献
87.
Berry V; Ionides AC; Moore AT; Plant C; Bhattacharya SS; Shiels A 《Human molecular genetics》1996,5(3):415-419
Inherited cataract is a clinically and genetically heterogeneous disease.
Here we report the identification of a new locus for an autosomal dominant
anterior polar cataract on the short arm of chromosome 17. To map this new
locus we performed genetic linkage analysis with microsatellite markers in
a four-generation pedigree. After exclusion of seven candidate loci for
cataract, we obtained significant positive LOD scores for markers D17S849
(Z = 4.01 / theta = 0.05) and D17S796 (Z = 4.17 / theta = 0.05). Multipoint
analysis gave a maximum LOD score of 5.2 (theta max = 0.06) between these
two markers. From haplotype analysis, the cataract locus lies in the 13 cM
interval between markers D17S849 and D17S796. This study provides the first
genetic mapping of an autosomal dominant anterior polar cataract.
相似文献
88.
89.
90.
Ann. Hum. Genet . (1999), 63 , 473–482
Correction
The authors wish to add the following correction to this paper:
The genomic organization of the human organic cation transporter (hOCT1/SLC22A1) has recently been described by us to consist of 7 exons [Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1( hOCT1 / SLC22A1 ); Ann. Hum. Genet . 63 : 473–482]. A reexamination revealed 11 exons instead of 7. The mistake occurred through cDNA contamination. The corrected gene structure of the hOCT1 gene is available at EMBL under the following accession numbers:
AJ243995 (Exon 1), AJ243996 (Exon 2), AJ276051 (Exon 3), AJ276052 (Exon 4), AJ276053 (Exon 5 and 6), AJ245460 (Exon 7), AJ243998 (Exon 8), AJ243999 (Exon 9 and 10) and AJ244000 (Exon 11). 相似文献
Correction
The authors wish to add the following correction to this paper:
The genomic organization of the human organic cation transporter (hOCT1/SLC22A1) has recently been described by us to consist of 7 exons [Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1( hOCT1 / SLC22A1 ); Ann. Hum. Genet . 63 : 473–482]. A reexamination revealed 11 exons instead of 7. The mistake occurred through cDNA contamination. The corrected gene structure of the hOCT1 gene is available at EMBL under the following accession numbers:
AJ243995 (Exon 1), AJ243996 (Exon 2), AJ276051 (Exon 3), AJ276052 (Exon 4), AJ276053 (Exon 5 and 6), AJ245460 (Exon 7), AJ243998 (Exon 8), AJ243999 (Exon 9 and 10) and AJ244000 (Exon 11). 相似文献