脉络宁高压氧治疗突发性聋

目的：观察脉络宁高压氧治疗突发性聋的疗效。方法：应用脉络宁20ml加入5％葡萄糖注射液或氯化钠注射液250ml中滴注,每日1次,连续使用1月,同时接受高压氧治疗,每日1次,10次为1疗程;并与西医治疗组进行对照。结果：脉络宁高压氧治疗突发性聋总有效率85．72％,显著高于西医治疗组有效率的54．10％;脉络宁高压氧治疗突发性聋的疗效与治疗时间的早晚有关,治疗愈早,疗效愈好,疗程与疗效无显著的相关性。结论：脉络宁高压氧治疗突发性聋,听力恢复快,无副作用,优于单纯西医治疗。     

大鼠去势后胸腺变化的组织学及超微结构的观察

目的：探讨去势对大鼠胸腺淋巴细胞超微结构与细胞功能的影响。方法：分别于去势大鼠术后2周、4周、6周、8周后,取胸腺组织进行HE染色和透射电镜观察。结果：实验表明去势导致胸腺淋巴细胞结构坏死,或者细胞器严重受损。结论：去势后胸腺淋巴细胞形态和功能均受到损害。     

慢性胰腺炎K—ras基因突变的临床病理学意义

目的：探讨慢性胰腺炎K－ras基因突变的临床病理学意义。方法：9例手术切除的慢性胰腺炎的石蜡标本,用微解剖法分离慢性胰腺炎的胰导管上皮粘液细胞增生灶,提取,扩增DNA,用ASO斑点杂交检测K－ras基因第12密码子的碱基序列。对9例接受手术治疗的病人进行长期随访,结果：9例慢性胰腺炎中4例有明显的胰导管上皮粘液细胞增生,其中2例被检出GAT型突变,分别占全组和有胰管上皮粘液细胞增生的22％和505,两个突变病例分别是接受了胰体尾切除和胰头十二指肠切除手术,术后分别随访17年和10年,没有发现任何恶变迹象,结构：慢性胰腺为可以检出K－ras基因突变,发生了K－ras基因突变的慢性胰腺炎上皮粘液细胞增生灶,未必一定发生为胰腺癌,胰液、粪便、末梢血液和尿液的检测,以及胰腺组织细针刺液检出K－ras基因突变,对胰腺癌的诊断有重要参考价值,但不应视为胰腺癌的确诊依据。     

鸡贫血病毒VP 3基因的克隆及其体外凋亡诱导效应的研究

用PCR方法扩增了鸡贫血病毒标准株的vp3基因,并将其克隆于真核表达载体pcDNA3上,构建了重组体pcDNA-vp3.经酶切鉴定及测序分析表明,该片段和预期相符.在体外利用LipofectAMINETM介导的基因转染,将pc-DNA-vp3、pcDNA3分别转入肝癌细胞系HepG2和二倍体肝细胞系L-02中,转染后的RT-PCR结果证实vp3基因在细胞中得到了表达.同时利用筛选稳定表达细胞株的技术和原位细胞凋亡检测法,证明了鸡贫血病毒是以凋亡的方式诱导细胞死亡,并且只诱导癌细胞的凋亡,而不诱导正常或二倍体细胞死亡.表明鸡贫血病毒vp3基因很可能成为一种极具潜力的抗肿瘤生物制剂.     

The association between plant-based diet and erectile function in Chinese young healthy men: A population-based study

The study was designed and conducted to evaluate the plant-based diet status and its association with erectile function (EF) in Chinese young healthy men. From July 2018 to May 2020, 116 objectively proved physically and psychologically healthy men were selected. Clinical questionnaires, detailed physical examinations and blood tests were all assessed. An overall plant-based diet index (PDI) and a healthful plant-based diet index (hPDI) were developed from each participant to measure plant-based diet quantitatively. The EF was measured by both the International Index of Erectile Function-5 (IIEF-5) and the surrogated measures of endothelial function, peripheral levels of nitric oxide (NO) and E-selectin. In subjects, the mean PDI and hPDI were 53.8 ± 8.2 and 54.7 ± 8.9 respectively. Results of multivariate analysis showed that both PDI and hPDI were negatively associated with BMI (all p < .05), but had no relationships with NO, E-selectin, TT levels and IIEF-5 scores (all p > .05). In a subgroup of male population, Chinese young healthy men, the plant-based diet, measured by PDI and hPDI, was not related to erectile function. The population can adhere to plant-based diet to keep healthy without concerns of negative influence on erectile function.     

Berberine plays a cardioprotective role by inhibiting macrophage Wnt5a/β-catenin pathway in the myocardium of mice after myocardial infarction

Myocardial infarction (MI) is one of the diseases with high fatality rate. Berberine (BBR) is a monomer compound with various biological functions. And some studies have confirmed that BBR plays an important role in alleviating cardiomyocyte injury after MI. However, the specific mechanism is unclear. In this study, we induced a model of MI by ligation of the left anterior descending coronary artery and we surprisingly found that BBR significantly improved ventricular remodeling, with a minor inflammatory and oxidative stress injury, and stronger angiogenesis. Moreover, BBR inhibited the secretion of Wnt5a/β-catenin pathway in macrophages after MI, thus promoting the differentiation of macrophages into M2 type. In summary, BBR effectively improved cardiac function of mice after MI, and the potential protective mechanism was associated with the regulation of inflammatory responses and the inhibition of macrophage Wnt5a/β-catenin pathway in the infarcted heart tissues. Importantly, these findings supported BBR as an effective cardioprotective drug after MI.     

Comparison between small renal masses 0-2 cm vs. 2.1-4 cm in size: A population-based study

BackgroundThe NCCN guidelines recommend active surveillance (AS) as an option for the initial management of cT1a 0-2 cm renal lesions. However, data about comparison between renal cell carcinoma (RCC) 0-2 cm vs. 2.1-4 cm are scarce.MethodsWithin the Surveillance, Epidemiology, and End Results database (2002–2016), 46,630 T1a N_anyM_any stage patients treated with nephrectomy were identified. Data were tabulated according to histological subtype, tumor grade (low [LG] vs. high [HG]), as well as age category and gender. Additionally, rates of synchronous metastases were quantified.ResultsOverall, 69.3 vs. 74.1% clear cell, 21.4 vs. 17.6% papillary, 6.9 vs. 6.8% chromophobe, 2.0 vs. 1.1% sarcomatoid dedifferentiation, 0.2 vs. 0.2% collecting duct histological subtype were identified for respectively 0-2 cm and 2.1-4 cm RCCs. In both groups, advanced age was associated with higher rate of HG clear cell and HG papillary histological subtype. In 0-2 cm vs. 2.1-4 cm RCCs, 13.8% vs. 20.2% individuals operated on harbored HG tumors and were more prevalent in males. Lower synchronous metastases rates were recorded in 0-2 cm RCC and ranged from 0 in respectively multilocular cystic to 0.9% in HG papillary histological subtype. The highest synchronous metastases rates were recorded in sarcomatoid dedifferentiation histological subtype (13.8% and 9.7%) in both groups.ConclusionsRelative to 2.1-4 cm RCCs, 0-2 cm RCCs harbored lower rates of HG tumors, lower rates of aggressive variant histology and lower rates of synchronous metastases. The indications and demographics of patients selected for AS may be expanded in the future to include younger and healthier patients.