Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3

PRL-3 is a newly identified nonclassical protein tyrosine phosphatase associated with colorectal cancer metastasis. To prepare specific monoclonal antibody (MAb) against PRL-3, cDNAs of PRL-1, -2, and -3 were cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli. Monoclonal antibody against PRL-3 was obtained with hybridoma technique and specific ELISA screening. Western blotting and immunohistochemistry assays showed that MAb 3B6 had specific binding ability with PRL-3 protein in eukaryotic cells. This MAb will be a helpful tool for the detection of PRL-3 protein in the tissues and serum of colorectal cancer and other cancer patients.     

Differential behavior of simple and complex cells in visual cortex during a brief IOP elevation

PURPOSE: To study and compare responses of different types of cortical neurons in the primary visual cortex in cats to grating stimuli before and during brief elevation of intraocular pressure (IOP). METHODS: Single-unit electrophysiological recordings were performed in anesthetized and paralyzed cats. The IOP was elevated by injecting saline into the anterior chamber of the cat's eyes through a syringe needle. The IOP was elevated to a level at which the retinal perfusion pressure (arterial pressure minus IOP) was maintained at approximately 30 mmHg for a period of 4 minutes. The responses of simple and complex cells in the primary visual cortex to visually drifting sinusoidal gratings were measured before and during the elevation of IOP. RESULTS: The response amplitude of all the cortical cells in the primary visual cortex declined during a brief elevation of IOP. The decrease in the response of simple cells was always more significant than that of complex cells. The differential decrease between the two major types of cells was independent of the cell's receptive field location and cortical depth. There was a mild tendency for cells with higher preferred spatial frequencies to be more sensitive than those with lower frequencies. The preferred orientation and direction of most cortical cells remained roughly unchanged though their orientation and direction biases decreased. An increase in the animal's blood pressure, which returned the retinal perfusion pressure to a normal level, compensated for the decreased response induced by the elevation of IOP. CONCLUSIONS: The differential effects of a brief elevation of IOP on the response of simple and complex cells in the visual cortex are general and may originate from the retina through the lateral geniculate nucleus (LGN), where different effects of elevation of IOP are exerted on X- and Y-type retinal ganglion cells. The results may suggest differential behavior of neurons tin the parvo and magno pathways of the primate.     

Parasagittal hemangioma associates with unilateral cheiro-oral-pedal syndrome

Cortical lesion might elicit restricted acral sensory deficit but a disparity of topographies in cheiro-oral-pedal syndrome is very rare. We report the first case of cheiro-oral-pedal syndrome due to a contralateral parasagittal hemangioma involving the supplement sensory area in parietal lobe. This unusual link between neuroanatomy and neurological feature is discussed.     

Combined free-tissue transfer for primary reconstruction of radial part of the hand

This paper presents 14 cases of primary reconstruction of radial-part hand defect by combined free-tissue transfer. The thumb was reconstructed by wraparound flap or second-toe transfer, while a lateral upper-arm flap or anterolateral thigh flap was transferred to cover the soft-tissue defect of the radial part of the hand. The arteries of the toe and flap were anastomosed and cascaded with the radial artery in an anatomical "snuffbox." Fourteen toes and 13 flaps survived in 14 cases. Only one flap was lost. Two-point discrimination of thumb tips was between 4-8 mm. The results suggest that combined free-tissue transfer appears to be a promising way of reconstructing a defect of the radial part of the hand.     

鞍区肿瘤围手术期防治下丘脑功能损害的临床研究

目的 探索鞍区肿瘤及其手术所致下丘脑功能损害的有效防治措施。方法 67例鞍区肿瘤需要手术的病人入院时被随机分为两组,研究组32例，实施我们制定的一系列围手术期防治下丘脑功能损害的措施。对照组35例,常规进行术前准备及术后处理,出现并发症后积极治疗.比较分析两组病例术后并发症的差异。结果 研究组尿崩症、血电解质紊乱、消化道出血、癫痫等的发生率及死亡率均较对照组低（ P〈0.05）。结论 鞍区肿瘤围手术期防治下丘脑功能损害可有效降低术后并发症的发生率和死亡率,是提高手术效果的重要措施。     

绝经妇女取环失败的原因及治疗措施

[目的]分析绝经后妇女取环失败原因及治疗措施。[方法]对本院2002年4月至2004年6月门诊132例绝经后不同原因所致取环失败者,给予尼尔雌醇片2mg每日1次口服,用药1周后取环,取环术中同时应用1%利多卡因作宫颈阻滞麻醉。[结果]132例中128例取出,4例因宫口闭锁,无法取出,成功率达93.93%,效果满意。[结论]对绝经后困难取环者,应用口服尼尔雌醇同时应用1%利多卡因作宫颈阻滞麻醉,可显著提高取环成功率,并减轻患者取环时不适感。     

参麦注射液联合PLF方案治疗乳腺癌的临床研究

目的:探讨参麦注射液联合PLF(双周疗法)方案治疗乳腺癌的疗效和不良反应。方法:将74例中晚期乳腺癌患者随机分为参麦注射液联合顺铂(DDP)加大剂量醛氢叶酸(HD-LV)加5氟脲嘧啶(5-FU)持续滴注48小时的PLF治疗组35;对照组单纯用PLF化疗39例,对其疗效及不良反应进行观察比较与分析,所有病人均接受2个疗程以上的治疗。结果:治疗组有效率(CR+PR)62.9%,对照组有效率53.8%,两者间差异无显著性(P>0.05);临床受益率治疗组85.7%,对照组64.1%,两者间差异有显著性(P<0.05);治疗组和对照组中位缓解期、中位生存期分别为(9.7±3.7)月、(8.4±2.6)月(P<0.05)和(12.9±4.4)月、(11.6±4.9)月(P>0.05);治疗组治疗后与治疗前相比CD3、CD4、CD8、CD4/CD8值有明显提高(P<0.05),而对照组治疗后较治疗前下降。两组的不良反应主要是外周静脉炎、骨髓抑制、消化道反应和脱发等,其不良反应相似,两组差异无显著性(P>0.05),单纯化疗组的恶心呕吐,心脏毒性和脱发(P<0.05)、白细胞减少更多见(P<0.01)。结论:参麦注射液联合PLF方案治疗乳腺癌可以改善肿瘤患者的生存质量和提高患者的免疫功能,减轻化疗所致的血液学毒性、心脏毒性和消化道反应,值得临床推广应用。     

针刺“足三里”对大鼠孤束核P物质的影响

目的 :观察延髓孤束核P物质免疫阳性反应产物的分布以及针刺“足三里”穴对其的影响。方法 :将SD大鼠随机分为三组 :电针“足三里”穴组 (n =10 )、电针“足三里”穴旁开 0 5cm组(n =10 )、空白对照组 (n =10 )。应用免疫组织化学ABC法显示延脑组织SP免疫活性切片。结果 :孤束核内P物质的免疫阳性反应产物以膨体型纤维为主 ,呈点状和串珠状 ,其敞开部有少量阳性胞体。主要分布于孤束核的内侧亚核、连合亚核 ,其余亚核内较为稀疏 ;电针“足三里”穴内侧亚核、连合亚核阳性纤维的光密度明显升高 ,其余亚核无明显变化 ;电针“足三里”穴外侧旁开 0 .5cm组与空白组相似 ,各亚核均无明显变化。结论 :针刺“足三里”可能通过抑制NTS内侧亚核和连合亚核SP的释放 ,对胃的功能活动产生兴奋作用     

利用生物芯片技术探讨支原体与胃癌的关系

目的：探讨支原体感染与胃癌的相关性以及胃癌细胞感染支原体后基因表达的变化。方法：建立组织芯片制备体系,制备包含105例胃癌组织、101例胃癌手术切缘正常组织和62例胃良性疾患的组织芯片。以猪鼻支原体特异抗体PD4为一抗,利用免疫组织化学的方法对组织芯片进行染色,判断猪鼻支原体感染的分布情况。借助基因芯片分析人胃癌细胞系MGC803细胞被支原体感染后基因表达水平的变化。结果：被测标本中,54．1％（53／98）的胃癌组织存在猪鼻支原体感染。胃癌标本正常切缘组织感染率51．7（45／87）。而胃良性疾患组织感染率仅为15．8％（9／57）,与胃癌组织相比差异有显著意义（P＝0．001）。感染率与肿瘤细胞分化程度成正比。人胃癌细胞NGC803细胞在感染支原体后,在被检测的48000个基因位点中共有409个基因的表达水平发生明显改变。部分调亡、细胞粘附相关基因的表达受到抑制。结论：胃癌组织中猪鼻支原体的感染率明显高于胃良性疾患组织,提示猪鼻支原体感染与胃癌的发生发展可能存在一定的相关性。猪鼻支原体的感染明显影响胃癌细胞的基因表达,提示猪鼻支原体的感染可能会影响胃癌细胞的生物学行为。