Detection of beryllium sensitivity using a flow cytometric lymphocyte proliferation test: the Immuno-Be-LPT

Measurement of lymphocyte proliferation to detect hypersensitivity to beryllium (Be-LPT) in vitro is done presently using a method based on tritiated thymidine incorporation. Although this method is sensitive it gives no information on cell viability or responding lymphocyte subsets. We have developed reliable and simple flow cytometric assays for lymphocyte proliferation testing (Immuno-Be-LPT) by combining immunophenotyping with bromodeoxyuridine (BrdU) incorporation or DNA content using propidium iodide (PI) or 4'6'-diimidazolin-2-phenylindole (DAPI). Evaluation of beryllium-induced lymphocyte proliferation in blood cells from seven patients with chronic beryllium disease (CBD) and 120 beryllium workers by both the Bc-LPT and the Immuno-Be-LPT showed agreement between the tests. The Immuno-Bc-LPT provided additional information about the specific type of lymphocytes responding. CD4+ lymphocytes proliferated in response to beryllium in blood samples from all seven CBD individuals and CD8+ lymphocytes proliferated in six of the seven. Four beryllium workers without CBD had positive responses to beryllium primarily in the CD8+ cells. The use of the individual's own plasma supported a greater beryllium or tetanus-induced proliferation of CD4+ lymphocytes when compared to commercial human serum. The response of CD4+ lymphocytes measured in the Immuno-Be-LPT may provide a new marker for the diagnosis of CBD.     

Cx基因在胃癌细胞株中的表达及诱导分析

目的：探讨胃癌细胞株ＭＧＣ８０３在全反式维甲酸（ａｌｌ－ｒａｎｓ－ｒｅｔｉｎｏｉｃ,ＲＡ）、佛波酯（ｐｈｏｒｂｌｏ ｅｓｔｅｒ,ＴＰＡ）及二甲基亚砜（ｄｉｍｅｔｈｙｌ ｓｕｌｆｏｘｉｄｅ,ＤＭＳＯ）３种诱导剂分别作用后,胃癌细胞中间隙连接蛋白（ｃｏｎｎｅｘｉｎ,Ｃｘ）基因表达变化。方法;采用比较多重ＲＴ－ＰＣＲ－Ｓｏｕｔｈｅｒｎ ｂｌｏｔ方法检测。结果：维甲酸、二甲基亚地胃癌细胞基因组中Ｃｘ４３基     

1964例原发性支气管肺癌的外科手术经验

目的 总结１９６４例原发性支气管肺癌的外科手术经验,方法 对１９６４例肺癌患者（中心型１１４０例,周围型８２４例）的临床和病理资料进行回顾性分析。结果 全组手术切除共１６２６例,切除率为８２．８％,其中全肺切除１６０例,肺叶切除１３８６例,支气管袖状成形肺叶切除７０例及支气管楔形成形肺叶切除１０例。术后发生并发症２９４例,死亡２０例,手术死亡率为１．０％（２０／１９６４）,对手术中常见的疑难病例处理进行了分析。结论 手术为肺癌的主要治疗方式之一。若遇左上肺产吕浸润和包绕左肺动脉干,应先阻断动脉根部,再分离被浸润的土叶肺动、静脉并予结扎切断,然后切除有肿瘤的左上叶,最后清除血管壁上残余的癌组织,中晚期中心型肺癌以及肺动,静脉根部距肿瘤距离太短（≤０．５ｃｍ）的病例应行心包内结扎肺血管。右上肺癌浸润和包绕上腔静脉和     

融合蛋白LHRH-PE40与癌细胞表面LHRH受体结合的特性

目的 :探讨重组毒素 LHRH- PE40与癌细胞表面 LHRH受体结合的特性。方法 :用放射性配基结合分析的方法测定亲和力和受体容量。结果 :LHRH- PE40与 Hela细胞的亲和力 :Kd=( 0 .36± 0 .1 2 ) nmol,容量 Bmax=( 0 .2 3± 0 .1 5 )μmol· g-1;与 Hep2细胞亲和力 :Kd=( 0 .33±0 .1 1 ) nmol,容量 Bmax=( 0 .46± 0 .1 2 ) μmol· g-1。结论 :重组毒素 LHRH- PE40保留了天然LHRH与其受体结合的高亲和力     

Clinical Usage of Laparoscopic Technique in Cerebrospinal Fluid Ventriculo-peritoneal Shunt

<正>Laparoscopic technique was used to place peritonesl terminal tube incerebrospinal fluid shunt in six patients with hydrocephalus in our series.Three kinds ofmethods were introduced.The results showed that the laparoscopic fine trocar puncture andsubcutaneous fixation method was the most efficient one.This approach was simple,reliableand less time-consuming.No complications associated with the technique were encountered.     

MR imaging of blood vessels using three-dimensional reconstruction: methodology

Multisection, dual-echo magnetic resonance (MR) transaxial images of blood vessels contain both anatomic and qualitative information about flow. Even so, the images are produced as a series of two-dimensional tomographic sections from which full visualization of connected structures is difficult. A computer algorithm was developed that automatically detects flowing blood based on pixel intensity and calculated T2 and provides reconstructed views of vessels while analyzing and displaying flow characteristics. Images of abdominal vessels, aortic aneurysms, and the heart were encoded by flow and color to demonstrate depth. In addition, these data were reconstructed to derive a more accurate assessment of patency. With this technique, transaxial images can be used to analyze flow patterns, determine patent areas, and visualize all levels of vessels in a single image.     

A prospective evaluation of treatment with Selective Internal Radiation Therapy (SIR-spheres) in patients with unresectable liver metastases from colorectal cancer previously treated with 5-FU based chemotherapy

Background  

To prospectively evaluate the efficacy and safety of selective internal radiation (SIR) spheres in patients with inoperable liver metastases from colorectal cancer who have failed 5FU based chemotherapy.