全文获取类型
收费全文 | 6569篇 |
免费 | 573篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 69篇 |
儿科学 | 254篇 |
妇产科学 | 129篇 |
基础医学 | 885篇 |
口腔科学 | 149篇 |
临床医学 | 668篇 |
内科学 | 1494篇 |
皮肤病学 | 259篇 |
神经病学 | 464篇 |
特种医学 | 394篇 |
外国民族医学 | 3篇 |
外科学 | 930篇 |
综合类 | 66篇 |
预防医学 | 468篇 |
眼科学 | 126篇 |
药学 | 432篇 |
1篇 | |
中国医学 | 20篇 |
肿瘤学 | 350篇 |
出版年
2023年 | 31篇 |
2022年 | 62篇 |
2021年 | 167篇 |
2020年 | 131篇 |
2019年 | 190篇 |
2018年 | 215篇 |
2017年 | 158篇 |
2016年 | 172篇 |
2015年 | 171篇 |
2014年 | 214篇 |
2013年 | 261篇 |
2012年 | 371篇 |
2011年 | 371篇 |
2010年 | 248篇 |
2009年 | 225篇 |
2008年 | 309篇 |
2007年 | 264篇 |
2006年 | 284篇 |
2005年 | 257篇 |
2004年 | 197篇 |
2003年 | 206篇 |
2002年 | 189篇 |
2001年 | 178篇 |
2000年 | 157篇 |
1999年 | 145篇 |
1998年 | 125篇 |
1997年 | 131篇 |
1996年 | 135篇 |
1995年 | 99篇 |
1994年 | 98篇 |
1993年 | 84篇 |
1992年 | 156篇 |
1991年 | 135篇 |
1990年 | 90篇 |
1989年 | 102篇 |
1988年 | 94篇 |
1987年 | 92篇 |
1986年 | 87篇 |
1985年 | 94篇 |
1984年 | 60篇 |
1983年 | 49篇 |
1982年 | 44篇 |
1981年 | 38篇 |
1980年 | 20篇 |
1979年 | 22篇 |
1978年 | 31篇 |
1976年 | 27篇 |
1974年 | 19篇 |
1970年 | 17篇 |
1969年 | 23篇 |
排序方式: 共有7161条查询结果,搜索用时 515 毫秒
61.
Response to a hepatitis B polypeptide vaccine in micelle form in a young adult population 总被引:2,自引:0,他引:2
F B Hollinger C Troisi D Heiberg Y Sanchez G R Dreesman J L Melnick 《Journal of medical virology》1986,19(3):229-240
Polypeptide micelles with relative molecular weights of 25,000 (p25) and 30,000 (gp30) daltons were prepared from native 22-nm hepatitis B surface antigen (HBsAg) particles. This p25/gp30 complex was alum-adsorbed, and three dosage levels (20 micrograms, 4 micrograms, and 0.8 micrograms) were administered at 0, 1, and 6 months to 51 human volunteers. Local and systemic reactions were clinically insignificant, and all vaccinees seroconverted, regardless of dose. As anticipated, antibody responses diminished as the dosage was reduced. Seroconversion rates and geometric mean antibody levels for the 20 micrograms dosage group were significantly better than those observed with a commercial vaccine and were comparable to those achieved after immunization with 40 micrograms of the intact 22-nm particles used to prepare the polypeptides. By 2 weeks, an anti-HBs response was elicited in 80% of the group receiving 20 micrograms of the polypeptide vaccine. This rapid response to immunization may be particularly beneficial for postexposure prophylaxis where the early development of immunity is advantageous. 相似文献
62.
R. Sanchez L. Kanarek J. Koninkx H. Hendriks P. Lintermans A. Bertels G. Charlier E. Van Driessche 《Microbial pathogenesis》1993,15(6)
Enterotoxigenic Escherichia coli strains expressing F17 fimbriae bind to the intestinal mucosa of young calves. F17 fimbriae recognize receptors present in the mucus layer and the brush-border membranes from duodenum, jejunum and ileum. The adhesion of E. coli F17 can be inhibited by several glycoproteins. Adhesion is also inhibited by pretreatment of mucus and brush-border membranes with sodium metaperiodate. The use of glycoconjugates as potential adhesion-blockers is further discussed. 相似文献
63.
Andersson J Sanchez J Ekdahl KN Elgue G Nilsson B Larsson R 《Journal of biomedical materials research. Part A》2003,67(2):458-466
Contact between blood and a biomaterial surface takes place in many applications and is known to activate the coagulation and complement systems. Heparin surface coatings have been shown to reduce blood activation upon contact with artificial surfaces. To establish the optimal heparin surface concentration, blood was incubated in a tubing loop model at 37 degrees C. The tubing was coated with different surface concentrations of heparin and rotated at three different velocities. We demonstrate that the blood compatibility of a surface with regard to coagulation, complement, and platelet activation can be improved by increasing the heparin surface concentration in the 6-12 pmol antithrombin/cm2 concentration interval. The binding of factor H is not influenced by the increased heparin surface concentration, suggesting that this factor is not the primary regulator of complement on heparin surfaces. In addition, the heparin coating has no effect on the complement activation that occurs on gas surfaces in extracorporeal circuits. 相似文献
64.
Domenico Rosace Cristina Gomez-Casado Paloma Fernandez Marina Perez-Gordo María del Carmen Dominguez Angel Vega María Teresa Belver Tania Ramos Francisco Vega Guadalupe Marco Manuel de Pedro Leticia Sanchez María de las Mercedes Arnas Marcela Santaolalla Miguel Ángel Saez Sara Benedé Montserrat Fernandez-Rivas Carlos Blanco Domingo Barber 《The Journal of allergy and clinical immunology》2019,143(2):681-690.e1
65.
66.
67.
Ana L Sanchez Carrie M Langdon Mahmood Akhtar Jun Lu Carl D Richards Premysl Bercik Derek M McKay 《Journal of interferon & cytokine research》2003,23(4):193-201
The use of biologics has promising potential in the treatment of inflammation. Studies with cultured cells and mouse models of disease have ascribed proinflammatory and anti-inflammatory functions to oncostatin M (OSM) and the related cytokine, interleukin-6 (IL-6). Here, we examined the effect of systemic administration of adenoviral (Ad) vectors encoding either murine OSM (AdMuOSM) or murine IL-6 (AdMuIL-6) in a mouse model of colitis. BALB/c mice were treated with a 5-day course of 4% dextran-sodium sulfate (DSS) water with or without administration of adenoviral vectors (i.p. or i.m. at 10(7) plaque-forming units [pfu]) given as a cotreatment or therapy. The deletion variant of the adenovirus served as a control for adenoviral infection. Colitis was assessed by (1) morphology (damage score, macrophage infiltration, apoptosis) and (2) function (myeloperoxidase activity and Ussing chamber analysis of epithelial ion transport). Infection with adenovirus alone did not affect colonic form or function. AdMuOSM (either i.p. or i.m.) significantly reduced the severity of the DSS-induced colitis. There was less damage, reduced macrophage infiltration, fewer apoptotic bodies, and a significant improvement in stimulated ion transport in colonic tissues from the treated mice. No benefit of AdMuIL-6 treatment was observed in this model system. Thus, systemic administration of AdMuOSM given as a cotreatment and to a lesser extent as a therapy was found to be of benefit in DSS-induced colitis, a murine model of inflammatory bowel disease (IBD). 相似文献
68.
69.
Expanded safety and immunogenicity of a bivalent, oral, attenuated cholera vaccine, CVD 103-HgR plus CVD 111, in United States military personnel stationed in Panama
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Taylor DN Sanchez JL Castro JM Lebron C Parrado CM Johnson DE Tacket CO Losonsky GA Wasserman SS Levine MM Cryz SJ 《Infection and immunity》1999,67(4):2030-2034
To provide optimum protection against classical and El Tor biotypes of Vibrio cholerae O1, a single-dose, oral cholera vaccine was developed by combining two live, attenuated vaccine strains, CVD 103-HgR (classical, Inaba) and CVD 111 (El Tor, Ogawa). The vaccines were formulated in a double-chamber sachet; one chamber contained lyophilized bacteria, and the other contained buffer. A total of 170 partially-immune American soldiers stationed in Panama received one of the following five formulations: (a) CVD 103-HgR at 10(8) CFU plus CVD 111 at 10(7) CFU, (b) CVD 103-HgR at 10(8) CFU plus CVD 111 at 10(6) CFU, (c) CVD 103-HgR alone at 10(8) CFU, (d) CVD 111 alone at 10(7) CFU, or (e) inactivated Escherichia coli placebo. Among those who received CVD 111 at the high or low dose either alone or in combination with CVD 103-HgR, 8 of 103 had diarrhea, defined as three or more liquid stools. None of the 32 volunteers who received CVD 103-HgR alone or the 35 placebo recipients had diarrhea. CVD 111 was detected in the stools of 46% of the 103 volunteers who received it. About 65% of all persons who received CVD 103-HgR either alone or in combination had a fourfold rise in Inaba vibriocidal titers. The postvaccination geometric mean titers were comparable among groups, ranging from 450 to 550. Ogawa vibriocidal titers were about twice as high in persons who received CVD 111 as in those who received CVD 103-HgR alone (600 versus 300). The addition of CVD 111 improved the overall seroconversion rate and doubled the serum Ogawa vibriocidal titers, suggesting that the combination of an El Tor and a classical cholera strain is desirable. While CVD 111 was previously found to be well tolerated in semiimmune Peruvians, the adverse effects observed in this study indicate that this strain requires further attenuation before it can be safely used in nonimmune populations. 相似文献
70.
Javier I. Escobar David J. Lakatua Barbara Streifel Norman L. Virnig Otto Sanchez 《Clinical genetics》1977,11(1):8-12
An abnormal chromosome No. 2 was found in the case of a child with an imperforate anus, a recto-vaginal fistula, unilateral atresia of the inner canal, and deformity of the external ear. G-banding studies revealed an insertion of a segment of the short arm into the long arm in one of the chromosomes No. 2 of the proband, the apparent result of a de novo phenomenon of chromosome rearrangement. 相似文献