全文获取类型
收费全文 | 600篇 |
免费 | 33篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 40篇 |
妇产科学 | 6篇 |
基础医学 | 52篇 |
口腔科学 | 33篇 |
临床医学 | 58篇 |
内科学 | 120篇 |
皮肤病学 | 17篇 |
神经病学 | 12篇 |
特种医学 | 101篇 |
外科学 | 75篇 |
综合类 | 24篇 |
预防医学 | 32篇 |
眼科学 | 4篇 |
药学 | 46篇 |
中国医学 | 1篇 |
肿瘤学 | 17篇 |
出版年
2022年 | 1篇 |
2021年 | 16篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 12篇 |
2015年 | 12篇 |
2014年 | 14篇 |
2013年 | 28篇 |
2012年 | 27篇 |
2011年 | 13篇 |
2010年 | 28篇 |
2009年 | 28篇 |
2008年 | 16篇 |
2007年 | 16篇 |
2006年 | 22篇 |
2005年 | 20篇 |
2004年 | 11篇 |
2003年 | 21篇 |
2002年 | 11篇 |
2001年 | 13篇 |
2000年 | 8篇 |
1999年 | 19篇 |
1998年 | 35篇 |
1997年 | 25篇 |
1996年 | 21篇 |
1995年 | 13篇 |
1994年 | 23篇 |
1993年 | 23篇 |
1992年 | 8篇 |
1991年 | 6篇 |
1990年 | 5篇 |
1989年 | 13篇 |
1988年 | 15篇 |
1987年 | 11篇 |
1986年 | 14篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 6篇 |
1982年 | 9篇 |
1981年 | 12篇 |
1980年 | 12篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 9篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1957年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有640条查询结果,搜索用时 9 毫秒
71.
72.
73.
X-linked syndrome of platelet dysfunction, thrombocytopenia, and imbalanced globin chain synthesis with hemolysis 总被引:6,自引:5,他引:1
An unusual family is described with a congenital bleeding disorder present in four males belonging to three generations. Of the three surviving affected males, all had splenomegaly and petechiae. The three had moderate thrombocytopenia (55-90 X 10(9)/liter) and markedly prolonged Ivy-template bleeding times (greater than 30 min). They were also noted to have reticulocytosis and, upon further investigation, imbalanced globin chain synthesis resembling that of beta-thalassemia minor. Studies on nine additional family members in four generations were normal except for slight elevations of reticulocyte counts in female members, one of whom had the abnormal globin chain synthesis ratio. In male members, the bleeding tendency and clinical signs always occurred in the presence of the globin chain synthesis defect and reticulocytosis. This previously undescribed condition was apparently transmitted as an X-linked disorder. 相似文献
74.
75.
Spinning top urethra (STU) is a term used to describe a widened posterior urethra seen mainly in girls. It is commonly regarded as a normal variant. The authors studied 30 girls with STU using videourodynamics. Twenty-eight showed bladder instability; 21, a congenital wide bladder neck anomaly; and 20, both instability and a wide bladder neck. One patient had a sensitive bladder. All patients had a urodynamic abnormality. The authors believe that the STU is nearly always an indication of bladder instability or wide bladder neck anomaly. The most common mechanism for the dilatation of the posterior urethra is that unstable contractions are resisted by a voluntary increase in distal sphincter tension so as to prevent leakage of urine. The resulting pressure rise produces distention of the posterior urethra, which will be maximal in subjects with a weak bladder neck mechanism as in the congenital wide bladder neck anomaly. The authors believe that STU is seldom if ever a normal variant. 相似文献
76.
The platelet release reaction was studied by a new quenched-flow approach. Platelets labeled with 14C-serotonin were reacted for short times (up to 5 sec) with thrombin and then quenched with glutaraldehyde or paraformaldehyde. Serotonin secretion began within 1 sec and was nearly complete by 4 sec. Aggregation recorded by a resistive-particle counter was similarly fast. Therefore, the quenched-flow system reveals that serotonin secretion can occur more rapidly than estimated in earlier studies. 相似文献
77.
G Zifarelli A Liantonio A Gradogna A Picollo G Gramegna M De Bellis AR Murgia E Babini D Conte Camerino M Pusch 《British journal of pharmacology》2010,160(7):1652-1661
Background and purpose:
ClC-K kidney Cl− channels are important for renal and inner ear transepithelial Cl− transport, and are potentially interesting pharmacological targets. They are modulated by niflumic acid (NFA), a non-steroidal anti-inflammatory drug, in a biphasic way: NFA activates ClC-Ka at low concentrations, but blocks the channel above ∼1 mM. We attempted to identify the amino acids involved in the activation of ClC-Ka by NFA.Experimental approach:
We used site-directed mutagenesis and two-electrode voltage clamp analysis of wild-type and mutant channels expressed in Xenopus oocytes. Guided by the crystal structure of a bacterial CLC homolog, we screened 97 ClC-Ka mutations for alterations of NFA effects.Key results:
Mutations of five residues significantly reduced the potentiating effect of NFA. Two of these (G167A and F213A) drastically altered general gating properties and are unlikely to be involved in NFA binding. The three remaining mutants (L155A, G345S and A349E) severely impaired or abolished NFA potentiation.Conclusions and implications:
The three key residues identified (L155, G345, A349) are localized in two different protein regions that, based on the crystal structure of bacterial CLC homologs, are expected to be exposed to the extracellular side of the channel, relatively close to each other, and are thus good candidates for being part of the potentiating NFA binding site. Alternatively, the protein region identified mediates conformational changes following NFA binding. Our results are an important step towards the development of ClC-Ka activators for treating Bartter syndrome types III and IV with residual channel activity. 相似文献78.
AR Jones BSC AJP Sandison FRCS WJ Owen MS FRCS 《International journal of clinical practice》1997,51(5):294-295
Pre-clerking of all patients undergoing elective general surgical operations was introduced at our hospital in an attempt to reduce an unacceptably high operation cancellation rate. A prospective audit has been performed on the effect of this policy on the cancellation rate. Before the introduction of pre-clerking there was a marked seasonal variation in the number of patients who failed to attend for surgery, which could be explained by absence on holiday. This seasonal variation disappeared after the start of pre-clerking clinics, but there has been no reduction in the number of cancellations for medical reasons. 相似文献
79.
A canine model of hemophilic (factor VIII:C deficiency) bleeding 总被引:8,自引:4,他引:8
A model of bleeding due to clotting factor deficiency has been developed in dogs. Normal and hemophilic (factor VIII:C deficient) animals were used. Bleeding was induced in lightly anesthetized animals by severing the apex of the nail cuticle using a guillotine device. In normal animals, bleeding usually ceased spontaneously after 2-8 min. In contrast, in hemophilic animals, bleeding continued for up to 20 min and necessitated either cauterization or the application of topical thrombin to achieve hemostasis. Pretreatment of the hemophilic animals with canine cryoprecipitate corrected the cuticle bleeding time to within the range noted for normal animals. The method is simple and reproducible and has the advantage that a number of observations can be made sequentially on the same animal. Rebleeding of the cauterized cuticle of the hemophilic animals did not usually occur. This model has considerable potential for the preclinical testing of products considered to bypass or replace factor VIII:C in patients with acquired inhibitors of factor VIII:C and may be adapted to the study of other mechanisms involved in normal and abnormal hemostasis. 相似文献
80.