全文获取类型
收费全文 | 4121篇 |
免费 | 280篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 155篇 |
妇产科学 | 91篇 |
基础医学 | 737篇 |
口腔科学 | 17篇 |
临床医学 | 397篇 |
内科学 | 931篇 |
皮肤病学 | 96篇 |
神经病学 | 450篇 |
特种医学 | 82篇 |
外科学 | 389篇 |
综合类 | 25篇 |
一般理论 | 1篇 |
预防医学 | 338篇 |
眼科学 | 75篇 |
药学 | 260篇 |
中国医学 | 3篇 |
肿瘤学 | 364篇 |
出版年
2024年 | 4篇 |
2023年 | 29篇 |
2022年 | 52篇 |
2021年 | 101篇 |
2020年 | 64篇 |
2019年 | 108篇 |
2018年 | 99篇 |
2017年 | 65篇 |
2016年 | 86篇 |
2015年 | 116篇 |
2014年 | 138篇 |
2013年 | 209篇 |
2012年 | 339篇 |
2011年 | 349篇 |
2010年 | 175篇 |
2009年 | 161篇 |
2008年 | 266篇 |
2007年 | 287篇 |
2006年 | 274篇 |
2005年 | 268篇 |
2004年 | 265篇 |
2003年 | 276篇 |
2002年 | 216篇 |
2001年 | 41篇 |
2000年 | 24篇 |
1999年 | 48篇 |
1998年 | 49篇 |
1997年 | 43篇 |
1996年 | 32篇 |
1995年 | 35篇 |
1994年 | 19篇 |
1993年 | 19篇 |
1992年 | 16篇 |
1991年 | 18篇 |
1990年 | 16篇 |
1989年 | 11篇 |
1988年 | 10篇 |
1987年 | 12篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1979年 | 8篇 |
1977年 | 4篇 |
1972年 | 6篇 |
1971年 | 2篇 |
1970年 | 4篇 |
1968年 | 6篇 |
1966年 | 2篇 |
排序方式: 共有4420条查询结果,搜索用时 0 毫秒
61.
Aurell H Etienne J Forey F Reyrolle M Girardo P Farge P Decludt B Campese C Vandenesch F Jarraud S 《Journal of clinical microbiology》2003,41(7):3320-3322
An analysis of 691 French clinical Legionella isolates showed that the endemic L. pneumophila serogroup 1 strain Paris was responsible for 12.2% of all cases of legionellosis and had a specific pulsed-field gel electrophoresis pattern. We also demonstrated the presence of this endemic clone throughout Europe. 相似文献
62.
Christelle Rivas Aouatef Djeraba Eugène Musset Nico van Rooijen Bas Baaten Pascale Quéré 《Avian pathology》2003,32(2):139-149
In this study the functional effectiveness of in vivo macrophage depletion using liposome-encapsulateddichloromethylene bisphosphonate (C12MBP) was examined in the chicken. The main target organs forsystemic liposome-encapsulated C12MBP treatment are the spleen and the liver. Intravenous treatment withC12MBP of B21/B21 chickens, genetically resistant to Marek's disease (MD), before challenge with the very virulent strain RB-lB, increased viral load in the blood and spleen after the first week and up to 6 weeks postinfection. In addition, C12MBP treatment dramatically increased tumour incidence and tumour load, especially in the spleens and livers of sick animals, but without affecting MD-specific mortality of B21/B21 cickens infected with RB-1B at 12 days of age. Nitric oxide (NO) is an important effector of the macrophage and has antiviral and antitumoural properties. NO has been shown to be one of the mechanisms triggered in resistance to Marek's disease. Intravenous treatment with Cl2MBP before infection with RB-1B induced a long-lasting decrease in numbers of macrophages and reduction in splenic inducible NO production associated with an absence of nitrate induction in the serum (up to 6 weeks pi). These results do not identify macrophage and NO production as major effector components in genetic resistance to Marek's disease, but underline their roles in limiting viraemia and tumour development in organs such as the spleen and the liver. 相似文献
63.
64.
65.
Alarms and Parameters Generated by Hematology Analyzer: New Tools to Predict and Quantify Circulating Sezary Cells 下载免费PDF全文
66.
Laure Calvet Aurélie Cabrespine Nathalie Boiret‐Dupré Etienne Merlin Catherine Paillard Marc Berger Jacques‐Olivier Bay Olivier Tournilhac Pascale Halle 《Transfusion》2013,53(3):570-578
BACKGROUND: Controlled‐rate freezing and storage in nitrogen is the standard technique for cryopreservation of peripheral hematopoietic progenitor cells (PHPCs) but presents high cost and dimethyl sulfoxide (DMSO) toxicity. Cryopreservation at ?80°C, by uncontrolled rate freezing with only 3.5% DMSO, preserves the functional capacities of PHPCs, produces successful engraftment, and reduces toxicity during infusion. STUDY DESIGN AND METHODS: Long‐term hematopoietic and immunologic reconstitution for 342 autografts (311 adults, 31 children) after PHPCs were cryopreserved at ?80°C was studied at 3, 6, and 12 months. The median (range) storage time of PHPCs cryopreserved was 1.7 (0.1‐5.99) months. RESULTS: Hemoglobin (Hb), white blood cells, and platelets (PLTs) reach normal values to trilineage at 12 months for 39% patients. Multivariate analysis shows a significant impact on CD34+ infused and on conditioning regimen for PLTs. Hb was influenced by growth factor administration at 3 months. Long‐term recovery is also highly dependent on blood counts (Hb, PLT, and neutrophil) at start of high‐dose chemotherapy. Only 43% of patients had reached normal lymphocyte values at 12 months after transplant, and a profound CD4+ T‐lymphocyte deficit remained, as others reported. CONCLUSION: Transplantation with PHPCs cryopreserved at ?80°C for no more than 6 months is satisfactory for long‐term hematopoietic and immunologic reconstitution, even if a profound CD4+ T lymphocyte deficit persists at 1 year. This easier and cheaper cryopreservation method also leads to successful engraftment. 相似文献
67.
De Vreese K Barylski R Pughe F Bläser M Evans C Norton J Semana G Holman R Loiseau P Masson D Gielis M De Brauwer A De Canck I Verpooten G Hulstaert F 《Clinical and diagnostic laboratory immunology》2004,11(2):430-432
We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests. 相似文献
68.
Magazin M Poszepczynska-Guigné E Bagot M Boumsell L Pruvost C Chalon P Culouscou JM Ferrara P Bensussan A 《The Journal of investigative dermatology》2004,122(1):111-118
Circulating malignant Sezary cells are a clonal proliferation of CD4+CD45RO+ T lymphocytes primarily involving the skin. To study the biology of these malignant T lymphocytes, we tested their ability to migrate in chemotaxis assays. Previously, we had shown that the neuropeptide neurotensin (NT) binds to freshly isolated Sezary malignant cells and induces through NT1 receptors the cell migration of the cutaneous T cell lymphoma cell line Cou-L. Here, we report that peripheral blood Sezary cells as well as the Sezary cell line Pno fail to migrate in response to neurotensin although they are capable of migrating to the chemokine stromal-cell-derived factor 1 alpha. This is in contrast with normal circulating CD4+ or CD8+ lymphocytes, which respond to both types of chemoattractants except after ex vivo short-time anti-CD3 monoclonal antibody activation, which abrogates the neurotensin-induced lymphocyte migration. Furthermore, we demonstrate that neurotensin-responsive T lymphocytes express the functional NT1 receptor responsible for chemotaxis. In these cells, but not in Sezary cells, neurotensin induces recruitment of phosphatidylinositol-3 kinase, and redistribution of phosphorylated cytoplasmic tyrosine kinase focal adhesion kinase and filamentous actin. Taken together, these results, which show functional distinctions between normal circulating lymphocytes and Sezary syndrome cells, contribute to further understanding of the physiopathology of these atypical cells. 相似文献
69.
Clinical and molecular delineation of Tetrasomy 9p syndrome: Report of 12 new cases and literature review 下载免费PDF全文
Laïla El Khattabi Sylvie Jaillard Joris Andrieux Laurent Pasquier Laurence Perrin Yline Capri Abdelmadjid Benmansour Annick Toutain Pascale Marcorelles Catherine Vincent‐Delorme Hubert Journel Catherine Henry Claire De Barace Louise Devisme Christèle Dubourg Florence Demurger Josette Lucas Marc‐Antoine Belaud‐Rotureau Jeanne Amiel Valérie Malan Marie‐Christine De Blois Loïc De Pontual Aziza Lebbar Nathalie Le DÛ Dominique P. Germain Jean‐Marc Pinard Eva Pipiras Anne‐Claude Tabet Azzedine Aboura Alain Verloes 《American journal of medical genetics. Part A》2015,167(6):1252-1261
70.
Mosaicism for Dominant Collagen 6 Mutations as a Cause for Intrafamilial Phenotypic Variability 下载免费PDF全文
Sandra Donkervoort Ying Hu Tanya Stojkovic Nicol C. Voermans A. Reghan Foley Meganne E. Leach Jahannaz Dastgir Véronique Bolduc Thomas Cullup Alix de Becdelièvre Lin Yang Hai Su Katherine Meilleur Alice B. Schindler Erik‐Jan Kamsteeg Pascale Richard Russell J. Butterfield Thomas L. Winder Thomas O. Crawford Robert B. Weiss Francesco Muntoni Valérie Allamand Carsten G. Bönnemann 《Human mutation》2015,36(1):48-56
Collagen 6‐related dystrophies and myopathies (COL6‐RD) are a group of disorders that form a wide phenotypic spectrum, ranging from severe Ullrich congenital muscular dystrophy, intermediate phenotypes, to the milder Bethlem myopathy. Both inter‐ and intrafamilial variable expressivity are commonly observed. We present clinical, immunohistochemical, and genetic data on four COL6‐RD families with marked intergenerational phenotypic heterogeneity. This variable expression seemingly masquerades as anticipation is due to parental mosaicism for a dominant mutation, with subsequent full inheritance and penetrance of the mutation in the heterozygous offspring. We also present an additional fifth simplex patient identified as a mosaic carrier. Parental mosaicism was confirmed in the four families through quantitative analysis of the ratio of mutant versus wild‐type allele (COL6A1, COL6A2, and COL6A3) in genomic DNA from various tissues, including blood, dermal fibroblasts, and saliva. Consistent with somatic mosaicism, parental samples had lower ratios of mutant versus wild‐type allele compared with the fully heterozygote offspring. However, there was notable variability of the mutant allele levels between tissues tested, ranging from 16% (saliva) to 43% (fibroblasts) in one mosaic father. This is the first report demonstrating mosaicism as a cause of intrafamilial/intergenerational variability of COL6‐RD, and suggests that sporadic and parental mosaicism may be more common than previously suspected. 相似文献