全文获取类型
收费全文 | 284篇 |
免费 | 12篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 14篇 |
妇产科学 | 9篇 |
基础医学 | 34篇 |
口腔科学 | 10篇 |
临床医学 | 32篇 |
内科学 | 50篇 |
皮肤病学 | 10篇 |
神经病学 | 2篇 |
特种医学 | 6篇 |
外科学 | 25篇 |
综合类 | 38篇 |
预防医学 | 26篇 |
眼科学 | 1篇 |
药学 | 28篇 |
中国医学 | 2篇 |
肿瘤学 | 18篇 |
出版年
2023年 | 5篇 |
2021年 | 10篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2016年 | 3篇 |
2015年 | 11篇 |
2014年 | 23篇 |
2013年 | 19篇 |
2012年 | 10篇 |
2011年 | 8篇 |
2010年 | 9篇 |
2009年 | 13篇 |
2008年 | 2篇 |
2007年 | 10篇 |
2006年 | 21篇 |
2005年 | 6篇 |
2004年 | 6篇 |
2003年 | 10篇 |
2002年 | 6篇 |
2001年 | 11篇 |
2000年 | 5篇 |
1999年 | 13篇 |
1998年 | 10篇 |
1997年 | 13篇 |
1996年 | 13篇 |
1995年 | 4篇 |
1994年 | 7篇 |
1993年 | 6篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1987年 | 3篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1973年 | 1篇 |
1966年 | 1篇 |
1964年 | 1篇 |
1959年 | 6篇 |
1958年 | 1篇 |
1957年 | 1篇 |
1956年 | 2篇 |
1955年 | 1篇 |
1954年 | 1篇 |
1952年 | 1篇 |
1941年 | 1篇 |
排序方式: 共有306条查询结果,搜索用时 15 毫秒
91.
风湿热是一种全身性炎症性疾病,其也可引起多系统器官的血管炎。结论:有风湿性二尖瓣病变及接受二尖瓣置换的患儿因冠脉系统血管炎而发生了心肌缺血,其中1例有神经系统表现,可能是由于脑部的血管炎引起的。儿童的风湿性心脏病和冠脉系统血管炎@Gunal N.$Department of Paediatr 相似文献
92.
目的:调查女性青少年中潜在的可改变因素如安全套使用、性行为和并发的性传播性疾病与生殖器人乳头状瘤病毒(H PV)感染持续时间的关系。设计:纵向观察研究。机构:在印第安纳州的印第安纳波利斯的3所诊所进行的研究。入选者:49名H PV阳性青少年对H PV感染进行经常性测试并提供性行为日记。主要调查内容:安全套使用情况、性行为、性伴侣人数和伴发的淋病奈瑟菌、沙眼衣原体和阴道毛滴虫感染。主要观察指标:对特定类型H PV感染从发生到清除的时间应用比例风险模型进行分析,应用校正后的危险比(AH Rs)来评价危险因素对H PV感染时程的影… 相似文献
93.
Martin EA; Heydon RT; Brown K; Brown JE; Lim CK; White IN; Smith LL 《Carcinogenesis》1998,19(6):1061-1069
A novel HPLC system has been developed that has allowed the separation of
tamoxifen DNA adducts formed in the livers of rats and mice treated with
this drug. At least 13 different peaks have been separated from
32P-post-labelled DNA, with two major peaks jointly accounting for >60%
of the total adducts formed by tamoxifen in the livers of treated rats and
mice. This is a great improvement on the resolution obtained by thin layer
chromatography, which separates the adducts into one main product
consisting of a group of major adduct spots eluting together, plus several
other minor spots. Identification of the nature of some of the peaks has
been investigated. Comparisons of the products formed when
alpha-acetoxytamoxifen is reacted with DNA in vitro with 32P-post- labelled
liver DNA adducts from rats treated with tamoxifen or alpha-
hydroxytamoxifen in vivo, appear to confirm that a major route of
activation of tamoxifen in vivo is via alpha-hydroxylation. The resolving
power of this HPLC system has further extended this result to show that six
of the peaks, including the two major peaks, are formed by the reaction of
an activated alpha-hydroxytamoxifen with DNA. Activation of
4-hydroxytamoxifen by the peroxidase/H2O2 system in vitro gives a more
polar DNA adduct seen only at trace levels in liver DNA from
tamoxifen-treated rats and mice.
相似文献
94.
目的:通过检测湖北地区极重度感音神经性聋患儿常见耳聋基因突变情况,分析该人群的分子病因学特点,为临床耳聋防治和遗传咨询提供参考。方法:收集306例湖北地区极重度感音神经性聋患儿,抽取外周血,提取DNA,应用遗传性耳聋基因芯片检测GJB2、GJB3、SLC26A4和线粒体12SrRNA4个基因的9个突变热点。对所有携带SLC26A4基因突变患者进行颞骨CT扫描。结果:306名患儿中,132例(43.14%)检出携带不同基因突变,其中有2例携带双基因突变。GJB2基因突变检出率为29.41%(90/306),SLC26A4基因突变检出率为13.72%(42/306),线粒体12SrRNA基因突变检出率为O.65%(2/306)。本组患者未检出GJB3基因突变。36例携带SLC26A4基因突变者颞骨CT扫描显示前庭水管扩大。结论:GJB2基因和SLC26A4基因是本组患儿最主要的致聋基因,其中235delC突变为最常见的突变位点,其次为1VS7—2A〉G突变。筛查SLC26A4基因常见突变有助于大前庭水管综合征的诊断。 相似文献
95.
目的 观察超声引导下微波凝固治疗肝癌前后患者免疫指标的动态变化。方法 分别于微波治疗前1周及治疗后1、2、3、4周采集患者外周静脉血,用Cr51释放法检测自然杀伤细胞活性,放射免疫法测定白细胞介素-2水平,酶联免疫法测定可溶性白介素2受体水平。结果 肝癌患者存在严重免疫功能低下,与正常人比NK细胞活性与IL-2水平下降;SIL-2R水平上升,均有统计学意义(P<0.01)。微波凝固治疗肝癌后,NK细胞活性明显上升(P<0.05),SIL-2R水平明显降低(P<0.01)。结论 微波热疗后机体免疫功能增强,提高了抗肿瘤能力。 相似文献
96.
Soohyeon Cho Lina Gu Ik Joon IN Bo Wu Taehoon Lee Hakwon Kim Sangho Koo 《RSC advances》2021,11(50):31511
One-pot conversion of sustainable d-ribose with l-amino acid, methyl esters produced pyrrole-2-carbaldehydes 5 in reasonable yields (32–63%) under pressurized conditions of 2.5 atm at 80 °C. The value-added pyrraline compounds 5 as platform chemicals were utilized for quick installation of poly-heterocyclic cores for the development of pyrrole-motif natural and artificial therapeutic agents. A pyrrole-fused piperazin-2-one scaffold 6 was prepared by reductive amination of pyrralines 5 with benzylamine. While further cyclization of pyrralines 5 with ethane-1,2-diamine produced pyrrolo-piperazin-2-ones 7 with an extra imidazolidine ring, the reaction with 2-amino alcohols derived from natural l-amino acids, alanine, valine, and phenylalanine, respectively provided pyrrolo-piperazin-2-ones 8, 9, and 10 with oxazolidine as the third structural core. Cell viability and an anti-inflammatory effect of the synthesized compounds were briefly tested by the MTT method and the Griess assay, among which 8h and 10g exhibited significant anti-inflammatory effects with negligible cell toxicity.One-pot conversion of sustainable d-ribose with l-amino acid, methyl esters produced pyrrole-2-carbaldehydes 5 in reasonable yields (32–63%) under pressurized conditions of 2.5 atm at 80 °C. 相似文献
97.
Neutrophil chemotaxis in acutely infected and clinically stable cystic fibrosis patients* 总被引:1,自引:0,他引:1
BURÇIN ener Gülen Hasçelik Uur Özçelk AYFER Günalp & AYHAN Göçmen 《Pediatrics international》1999,41(5):514-518
Abstract Purpose : The aim of the present study was to evaluate the role of neutrophil chemotaxis in cystic fibrosis (CF) and to also determine whether an acute bacterial infection and the nutritional status of a child can affect neutrophil chemotaxis.
Methods : Twelve acutely infected and 12 clinically stable CF patients and 10 healthy age-matched controls were studied. Neutrophil chemotaxis and random migration were investigated in vitro in the peripheral blood of subjects by the Boyden chamber method and the results were expressed as chemotactic index (CI). The nutritional status of the cases was evaluated as body mass index (BMI).
Results : The CI values in the acutely infected group were found to be significantly lower than the clinically stable and healthy control groups ( P <0.05 and P <0.005, respectively). There was no significant difference between the clinically stable CF group and the healthy control group ( P >0.1). No significant correlation was detected between the CI and BMI of the two groups of CF patients ( P >0.05).
Conclusions : The present study confirms that neutrophil chemotaxis and random migration are normal in clinically stable CF patients. The decreased CI in the acutely infected patients indicates the possible role of infection itself on neutrophil chemotaxis. 相似文献
Methods : Twelve acutely infected and 12 clinically stable CF patients and 10 healthy age-matched controls were studied. Neutrophil chemotaxis and random migration were investigated in vitro in the peripheral blood of subjects by the Boyden chamber method and the results were expressed as chemotactic index (CI). The nutritional status of the cases was evaluated as body mass index (BMI).
Results : The CI values in the acutely infected group were found to be significantly lower than the clinically stable and healthy control groups ( P <0.05 and P <0.005, respectively). There was no significant difference between the clinically stable CF group and the healthy control group ( P >0.1). No significant correlation was detected between the CI and BMI of the two groups of CF patients ( P >0.05).
Conclusions : The present study confirms that neutrophil chemotaxis and random migration are normal in clinically stable CF patients. The decreased CI in the acutely infected patients indicates the possible role of infection itself on neutrophil chemotaxis. 相似文献
98.
rhBMP-2对骨骼肌卫星细胞增殖与粘附的影响 总被引:1,自引:2,他引:1
目的:探讨人重组骨形态发生蛋白(rhBMP)一2对骨骼肌卫星细胞增殖与粘附的影响。方法:体外分离与培养骨骼肌卫星细胞,分别用0、50、100、500、1000ng/ml的rhBMP-2诱导培养基培养48h。利用MTT法测定细胞增殖能力的变化,通过荧光法测定接种后1h的粘附细胞率。结果:rhBMP-2可促进骨骼肌卫星细胞的增殖,这种作用从BMP浓度为500ng/ml即可表现出来,并随着浓度的增加而越发明显。在rhBMP-2作用下骨骼肌卫星细胞的粘附率增高,在500ng/ml的浓度时达最高,但当BMP浓度进一步增加时,细胞粘附率却不再增加。结论:rhBMP-2可促进骨骼肌卫星细胞的增殖,增强其粘附特性。 相似文献
99.
100.
Vladimir GAPAROVI Radovan RADONI Mirko GJURAIN Hrvoje GAPAROVI Dragutin IVANOVI Marijan MERKLER Ivan JELIC 《Nephrology (Carlton, Vic.)》1997,3(2):155-158
Summary: The aim of our study was to explore the aetiology and the outcome of acute renal failure (ARF) during the war in Croatia. of the 2132 patients admitted to our hospital between April 1990 and November 1992 due to war related trauma, 11 (0.5%) developed ARF. We believe that the development of ARF in these patients was secondary to an overwhelming septic process. Most of our patients suffered from multiple organ failure. of the 11 patients suffering from ARF due to war related trauma only four recovered (63.6% had died). We attribute the lethal outcome to the progression of the septic process. Patients who developed ARF due to infectious diseases unrelated to trauma had a different prognosis. Acute renal failure caused by the Hantan virus ran a benign course, in both its oliguric and non-oliguric form. Patients who developed ARF as a complication of leptospirosis also had a good prognosis. Although ARF is usually of a multifactorial genesis, our study aimed to emphasize the importance of disseminated septic processes as a cause of ARF. 相似文献