Dedifferentiated liposarcoma (DDLPS) in the rectum: A case report

Dedifferentiated liposarcoma (DDLPS) is a rare subtype of liposarcoma with a poor prognosis. This current case report describes a rectal DDLPS in a 68-year-old Chinese male that presented with lower abdominal pain and weight loss. Computed tomography and magnetic resonance imaging were undertaken to evaluate the tumour. The patient underwent radical resection of the rectal tumour, sigmoid colostomy and partial ureterectomy. The tumour was positive for mouse double minute 2 by immunohistochemistry. The patient healed well but refused chemotherapy postoperatively for economic reasons. The tumour recurred and metastasized 4 weeks after the operation. After relevant treatment, the patient''s condition deteriorated and he died of shock, metabolic acidosis, hyperlactataemia and acute renal failure. The case report also reviews the literature in terms of the clinical diagnosis, treatment and pathological characteristics of rectal DDLPS with the aim of improving the level of diagnosis and treatment.     

Precision N‐glycoproteomics reveals elevated LacdiNAc as a novel signature of intrahepatic cholangiocarcinoma

Primary liver cancer, mainly comprising hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC), remains a major global health problem. Although ICC is clinically different from HCC, their molecular differences are still largely unclear. In this study, precision N‐glycoproteomic analysis was performed on both ICC and HCC tumors as well as paracancer tissues to investigate their aberrant site‐specific N‐glycosylation. By using our newly developed glycoproteomic methods and novel algorithm, termed ‘StrucGP’, a total of 486 N‐glycan structures attached on 1235 glycosites were identified from 894 glycoproteins in ICC and HCC tumors. Notably, glycans with uncommon LacdiNAc (GalNAcβ1‐4GlcNAc) structures were distinguished from their isomeric glycans. In addition to several bi‐antennary and/or bisecting glycans that were commonly elevated in ICC and HCC, a number of LacdiNAc‐containing, tri‐antennary, and core‐fucosylated glycans were uniquely increased in ICC. More interestingly, almost all LacdiNAc‐containing N‐glycopeptides were enhanced in ICC tumor but not in HCC tumor, and this phenomenon was further confirmed by lectin histochemistry and the high expression of β1‐4 GalNAc transferases in ICC at both mRNA and protein expression levels. The novel N‐glycan alterations uniquely detected in ICC provide a valuable resource for future studies regarding to the discovery of ICC diagnostic biomarkers, therapeutic targets, and mechanism investigations.     

不同浓度含钠电解质溶液复苏对烧伤小儿机体内环境的影响

目的观察两种不同含钠浓度的电解质溶液对小儿烧伤液体复苏后机体内环境的影响。方法选择40例年龄6个月至两岁的小儿烧伤病例,烧伤面积从25%～54%,随机分成俩组,每组20例,A组采用平衡盐溶液(A组含钠量130mmol/L),B组采用高钠复方乳酸钠溶液(含钠量174mmol/L),两种不同的电解质溶液行液体复苏。并分别于伤后1～4天抽取血标本检测K 、Na 、Cl-和HCO3-四种电解质,记录各时段液体输入量和尿量以及血浆晶体渗透压等。结果A组伤后第一天的补液量明显高于B组,且A组血清Na 、HCO3-浓度明显下降,伤后第二天逐步回升。A、B两组K 浓度于伤后第三、第四天明显下降。结论电解质溶液中钠离子浓度的不同对小儿烧伤机体内环境的影响较大,含钠量高的溶液可能更有利于烧伤小儿机体内环境保持稳定。     

小鼠骨髓间充质干细胞的分离培养及生物性状研究

目的：分离培养小鼠骨髓间充质干细胞，研究其体外培养的生物特性。方法：获取骨髓间充质干细胞进行培养，倒置相差显微镜观察细胞生长情况。绘制生长曲线，冷冻保存细胞复苏后的生长状况观察。免疫细胞化学法鉴定细胞表面抗原。在地塞米松、β-甘油磷酸钠、抗坏血酸的作用下，进行成骨诱导分化。结果：原代和传代培养的细胞体外培养细胞呈现梭形、多角形外观，具有较强的生长增殖能力，复苏细胞生长状况无明显改变；细胞CD44。CD54抗原有阳性表达，CD34呈阴性表达。分化细胞表现成骨细胞特性，合成碱性磷酸酶(ALP)能力增强，矿化结节逐渐出现。结论：建立稳定可靠的分离培养小鼠骨髓MSCs方法，分离出的MSCs具有干细胞的生物特性，可用于骨组织工程中的种子细胞。     

Lactiplantibacillus plantarum-12 Alleviates Inflammation and Colon Cancer Symptoms in AOM/DSS-Treated Mice through Modulating the Intestinal Microbiome and Metabolome

In our previous research, Lactiplantibacillus plantarum-12 alleviated inflammation in dextran sodium sulfate (DSS)-induced mice by regulating intestinal microbiota and preventing colon shortening (p < 0.05). The purpose of the present study was to evaluate whether L. plantarum-12 could ameliorate the colon cancer symptoms of azoxymethane (AOM)/DSS-treated C57BL/6 mice. The results showed that L. plantarum-12 alleviated colonic shortening (from 7.43 ± 0.15 to 8.23 ± 0.25) and weight loss (from 25.92 ± 0.21 to 27.75 ± 0.88) in AOM/DSS-treated mice. L. plantarum-12 oral administration down-regulated pro-inflammatory factors TNF-α (from 350.41 ± 15.80 to 247.72 ± 21.91), IL-8 (from 322.19 ± 11.83 to 226.08 ± 22.06), and IL-1β (111.43 ± 8.14 to 56.90 ± 2.70) levels and up-regulated anti-inflammatory factor IL-10 (from 126.08 ± 24.92 to 275.89 ± 21.87) level of AOM/DSS-treated mice. L. plantarum-12 oral administration restored the intestinal microbiota dysbiosis of the AOM/DSS treated mice by up-regulating beneficial Muribaculaceae, Lactobacillaceae, and Bifidobacteriaceae levels and down-regulating pathogenic Proteobacteria, Desulfovibrionaceae, and Erysipelotrichaceae levels. As a result, the fecal metabolites of the AOM/DSS-treated mice were altered, including xanthosine, uridine, 3,4-methylenesebacic acid, 3-hydroxytetradecanedioic acid, 4-hydroxyhexanoylglycine, beta-leucine, and glycitein, by L. plantarum-12 oral administration. Furthermore, L. plantarum-12 oral administration significantly ameliorated the colon injury of the AOM/DSS-treated mice by enhancing colonic tight junction protein level and promoting tumor cells death via down-regulating PCNA (proliferating cell nuclear antigen) and up-regulating pro-apoptotic Bax. (p < 0.05). Taken together, L. plantarum-12 oral administration could ameliorate the colon cancer burden and inflammation of AOM-DSS-treated C57BL/6 mice through regulating the intestinal microbiota, manipulating fecal metabolites, enhancing colon barrier function, and inhibiting NF-κB signaling. These results suggest that L. plantarum-12 might be an excellent probiotic candidate for the prevention of colon cancer.     

Pituitary deficiency due to primary pituitary apoplexy

To the editor： Primary pituitary apoplexy （PA） has few been reported in the cases of adolescents by any English publication.Unlike the secondary PA, primary PA often occurs in patients with pituitary tumor. It is rare and subclinical. Here we report an extremely rare case of chronic primary PA. The patient developed pituitary deficiency with symptoms like delay of puberty and low level of hormone. Therefore, we present the hormone characteristics, imaging findings and the briefly review other literature.