彩色多普勒与其它检查方法对心尖肥厚型心肌病诊断价值的比较

目的 :探讨CDFI对AHCM的诊断价值。材料和方法 :对经ECG、MRI、CIN和EMB检查确诊的AHCM的CD FI特点与诊断及鉴别诊断方法进行分析、讨论。结果 :分别由上述检测技术提示或证实的 2 1例AHCM患者均显示乳头肌以下心室游离壁 /室间隔不同程度的增厚。当厚度 >1 5cm以上者 ,心室收缩时 ,室腔区可消失。 2 1例中有 2例合并有右室心尖肥厚。结论 :CDFI为AHCM最理想的检测方法。如结合以上其它检查 ,特别是ECG提示 ,则可避免假阴性的出现 ,进一步提高准确性     

硬膜外血肿并天幕疝的CT研究

目的 分析硬膜外血肿并天幕疝的CT表现，探讨血肿体积与天幕疝程度的关系。资料与方法 对86例硬膜外血肿患者应用斜坡垂线扫描法进行研究。结果 斜坡垂线扫描图像上，36例显示天幕疝，其CT表现为：与颞叶脑组织相连的小脑幕内侧环池内软组织密度影。颞叶疝至幕下3mm以内者4例，3－6mm者12例，6mm以上者20例，相应血肿体积分别为30.99-34.81ml、32.84-41.76ml和39．15－69．54ml。结论 应用斜坡垂线扫描法，CT可对硬膜外血肿合并天幕疝作出准确诊断；血肿体积与疝入程度有关。     

曲马多预防硬膜外麻醉后寒战的临床观察

目的　观察曲马多对硬膜外麻醉后寒战的预防作用。方法　 6 0例硬膜外麻醉患者随机分为曲马多组 (Ⅰ组 ,n =2 0 )、哌替啶组 (Ⅱ组 ,n =2 0 )和对照组 (Ⅲ组 ,n =2 0 )。记录三组麻醉后寒战的发生情况和 30min内SpO2 的变化及三组并发症的发生情况。结果　Ⅰ ,Ⅱ组仅有 1例发生寒战 ,而Ⅲ组有 15例寒战。与Ⅲ组相比 ,Ⅱ组SpO2 下降明显 (P <0 .0 5 ) ,Ⅰ组变化不明显 (P >0 .0 5 )。结论　预防性应用曲马多可以有效预防硬膜外麻醉后寒战     

西藏土壤厌氧芽胞梭菌的调查分析

目的　了解西藏高原土壤厌氧芽胞细菌的种类、数量及其对药物敏感情况 ,为平战时感染提供诊疗依据。方法　按照Bergey’s手册对土样进行厌氧培养分离及生化反应鉴定。结果　每克土壤中厌氧芽胞梭菌数为 :30～ 1 6 2 0 0 0、细菌总数为 :0 .1 1× 1 0 7～ 88.6× 1 0 7;同一地区不同地点、同一地点不同深度细菌数和梭菌数相差甚大。未开垦地区不能培养出厌氧细菌 ,75cm以下不能检出厌氧细菌。分离出的 2 7种 93株厌氧芽胞梭菌中主要为产气荚膜梭菌占 2 0 4 %。结论　西藏地区土壤存在有能产生气性坏疽的厌氧芽胞梭菌及其它厌氧菌 ,但大部分来自人或动物的粪便 ,其对常用抗生素的敏感率均在 90 %以上     

严重创伤早期大鼠下丘脑HSP70与iNOS表达与分布的时效关系

目的研究严重创伤早期大鼠下丘脑热休克蛋白(HSP)70与诱导型一氧化氮合酶(iNOS)转录表达变化分布和意义.方法采用BIM-Ⅲ型生物撞击机致大鼠胸部严重撞击伤,并造成单侧股骨骨折,运用S-ABC免疫组化、原位杂交技术测定下丘脑HSP70与iNOS及其mRNA的转录表达水平.结果正常对照组HSP70低水平表达,在伤后1小时即开始明显增高(P＜0.05),6小时达到峰值为正常的18倍,12小时后开始下降,24小时时主要集中在室旁核内;iNOS无基础表达,在伤后1小时开始出现,随HSP70同步增高,8小时达到高峰,较1小时时增加13倍.两者相关系数r=0.97601.结论严重创伤后早期下丘脑内HSP70和iNOS过度表达, 在严重创伤应激时下丘脑的损伤与抗损伤机制中起重要作用.     

放射线联合p53基因及内皮抑素治疗小鼠前列腺癌皮下移植瘤

Objective To test the hypothesis that p53 gene therapy combined with endostatin can enhance tumor response to radiation therapy of RM-1 mouse xenograft prostate cancer and to investigate its mechanism. Methods A mouse prostate cancer model was established. Then mice with xenograft tumor were randomly divided into group A (control), B (radiation), C (radiation and rAdp53), D (radiation and rh-endostatin) and E (radiation and rAdp53 and rh-endostatin). On day 1, rAdp53 was injected intra-tumorously with 1 × 1010 vp per animal to group C and E. From day 1 to 14, rh-endostatin was given 15 mg/kg intraperitoneally daily to group D and E. On day 4 single fraction of 15 Gy was given to tumors in groups B, C, D and E. Normal saline was injected intra-tumorously or intraperitoneaUy accordingly as control. No treatment was done to group A. Tumor volume was measured daily. Samples were collected on Days 5, 10 and 15. Ki67, CD31, p53 and VEGF were detected by means of immunohistochemistry. Results (1) Radiation alone, radiation combined with intra-tumorous injection of Adp53 and/or intraperitoneal injection of rh-endostatin resulted in tumor growth arrest of RM-1 cells in vivo (P = 0.000). Radiation combined with both rAdp53 and rh-endostatin was the most effective treatment (P < 0.05). (2) All the four treatment groups had a decreased expression of mutant type P53 (P = 0.000). The expression of Ki67 in groups B and C were equal (P 0.05) and increasing (P = 0.000), respectively. Group D had a up-down-up curve (P < 0.05), but group E had a up-down one. On day 5 the expresion of VEGF in group E was the lowest (P < 0.05). An increased expression of MVD compared with the control was shown, and MVD in groups C, D and E were always higher than that in the control (P < 0.05). Conclusions The limitation of radiotherapy could be overcome by combination with beth p53 gene therapy and endostatin on the growth of mouse prostate cancer cell. Radiation, rAdp53 and endostatin have their own role but they can be interacted with each other.     

Determination of the prognostic value of [(18)F]fluorodeoxyglucose uptake by using positron emission tomography in patients with non-small cell lung cancer

The aim of this study was to determine whether quantitative information obtained from [(18)F]fluorodeoxyglucose positron emission tomography ((18)F-FDG PET) has a prognostic significance for patients with non-small cell lung cancer (NSCLC). We investigated (18)F-FDG PET imaging of 73 patients with NSCLC. The maximum standardized uptake value (SUV(max)) was significantly different between the histopathological types of tumour (squamous cell carcinoma (n=37, 12.4+/-5.1), adenocarcinoma (n=30, 8.2+/-5.8), bronchioloalveolar carcinoma (n=4, 2.6+/-1.7), <0.01). In the univariate analysis of all patients, staging (P=0.0001), tumour cell type (P=0.013), and a SUV(max) greater than 7 (P=0.0011) was correlated with survival. However, a multivariate analysis identified staging and SUV(max) greater than 7 were affected survival adversely. The mortality rate of patients with group I disease (stage I to stage IIIA) was 5.8 times lower than that of patients with group II disease (stage IIIB to stage IV). Patients with a high SUV(max) (> or =7) had a 6.3 times higher mortality than those with a low SUV(max)(<7). By multivariate analysis of patients with squamous cell carcinoma, only grouping affected survival (P=0.008, relative risk=4.3). In the case of adenocarcinoma, the SUV(max) (>10) correlated exclusively with poorer survival (P=0.031, relative risk=11.152). (18)F-FDG uptake correlated with survival in NSCLC. Especially in adenocarcinomas, the SUV(max) was complementary to other known prognostic factors.     

Contrast-enhanced power Doppler US: is it useful in the differentiation of gallbladder disease?

Thirteen patients with gallbladder disease underwent power Doppler ultrasound (PDUS) before and after microbubble contrast agent injection. Lesion and liver bed vascularity was evaluated. Pathological diagnoses in nine patients were two acute cholecystitis, four chronic inflammation, one adenoma and two adenocarcinoma. Two cases of cancer were included on clinical and radiological findings. Two cases were excluded because no pathologic diagnosis was available. Liver bed hyperemia was noted only in acute cholecystitis. Contrast-enhanced PDUS was superior to nonenhanced PDUS in the demonstration of vascularity of gallbladder diseases. However, contrast-enhanced PDUS has limited value in the differentiation.     

70%肝切除对大鼠胎肝细胞脾内移植后增殖能力的影响

研究70％肝切除对大鼠胎肝细胞脾内移植后增殖影响。分离孕3周SD大鼠胚胎肝细胞，将其移植入70％肝切除大鼠脾内，分别于移植后7天和30天应用流式细胞仪分析肝切除大鼠残肝细胞的细胞周期，用图像分析法检测脾内移植肝细胞面积密度。与对照组比较，胎肝细胞移植后7天，肝切除鼠残细胞S期细胞比例明显增加（P＜0．05），Gz/M期细胞比例明显减少（P＜0．01）。而其牌内移植 胎肝细胞面积密度则显著升高（P＜0．05）；30天后，残 细胞再生状态与移植胎肝细胞的面积密度与对照组比较均无显著性差异。研究表明，70％肝切除的肝再生有利于大鼠肝细胞脾内移植后的增殖。     

创伤性湿肺的X线与CT诊断

目的 探讨X线摄影、CT在创伤性湿肺诊断中的应用价值及其临床意义。方法 回顾分析21例创伤性湿肺病例的X线、CT影像资料，并加以总结。结果 创伤性湿肺主要表现为肺纹影增强、模糊，占85.71%；肺内斑片影，占71.43%；面纱征或／和毛玻璃征，占23.81%；肺血肿影，占14.29%及其他如肋骨骨折、胸腔积液、膈肌破裂等影像表现。结论 X线摄影、CT检查目前仍是诊断创伤性湿肺的主要手段。