榄香烯乳联合洛铂腹腔热灌注治疗中晚期结直肠癌恶性腹水的临床研究

目的 探讨榄香烯乳联合化疗药物洛铂腹腔热灌注对中晚期恶性结直肠癌腹水的临床疗效.方法 对2017年1月至2019年12月,广州医科大学附属肿瘤医院诊断为中晚期大肠癌并伴恶性腹水的患者共40例,按照入组条件,遵循抛硬币法分为治疗组(20例),对照组(20例),对照组予单药洛铂(50 mg),治疗组予洛铂(50 mg)加中...     

��ͯ�������������Լ���

根据罗马Ⅲ标准,儿童上消化道功能性疾病包括呕吐和吞气症(青少年反刍综合征、周期性呕吐综合征、吞气症),腹痛相关的功能性胃肠病(功能性消化不良)。本文主要阐述周期性呕吐综合征和功能性消化不良。1小儿周期性呕吐综合征近年儿科界对周期性呕吐综合征(cyclic vomiting syn-drome,CVS)的定义[1]为:3次或更多次的发作性的顽固的恶心和呕吐,每次发作持续数小时至数日,2次发作间有长达数周至数月的完全无症状的间歇期。患者不存在任何代谢、神经、胃肠等各系统的异常。1.1流行病学[1-3]CVS确切的流行病学尚未完全清楚。20世纪60年代报道为…     

Resistin induces insulin resistance, but does not affect glucose output in rat-derived hepatocytes

AIM: The aim of the present study was to observe the effects of resistin on insulin sensitivity and glucose output in rat-derived hepatocytes. METHODS: The rat hepatoma cell line H4IIE was cultured and stimulated with resistin; supernant glucose and glycogen content were detected. The insulin receptor substrate (IRS)-1 and IRS-2, protein kinase B/Akt, glycogen synthase kinase-3beta(GSK-3 beta), the suppressor of cytokine signaling 3 (SOCS-3) protein content, as well as the phosphorylation status were assessed by Western blotting. Specific antisense oligodeoxynucleotides directed against SOCS-3 were used to knockdown SOCS-3. RESULTS: Resistin induced insulin resistance, but did not affect glucose output in rat hepatoma cell line H4IIE. Resistin attenuated multiple effects of insulin, including insulin-stimulated glycogen synthesis and phosphorylation of IRS, protein kinase B/Akt, as well as GSK-3beta. Resistin treatment markedly induced the gene and protein expression of SOCS-3, a known inhibitor of insulin signaling. Furthermore, a specific antisense oligodeoxynucleotide directed against SOCS-3 treatment prevented resistin from antagonizing insulin action. CONCLUSION: The major function of resistin on liver is to induce insulin resistance. SOCS-3 induction may contribute to the resistin-mediated inhibition of insulin signaling in H4IIE hepatocytes.     

自发性大脑凸面蛛网膜下腔出血合并急性脑梗死的病因及临床特点

目的 探讨自发性大脑凸面蛛网膜下腔出血(cSAH)合并急性脑梗死(AIS)的病因及临床特点.方法 回顾性分析2012年1月至2020年1月在北京市顺义区医院神经内科住院的160例cSAH患者的临床资料,根据是否合并AIS,将患者分为cSAH组和cSAH合并AIS组.采用SPSS 19.0软件进行统计分析,根据数据类型,...     

健康促进对产后抑郁症患者生活质量影响的研究

目的 探讨健康促进对产后抑郁症患者生活质量的影响.方法 将182例产后抑郁症患者随机分为实验组与对照组,每组各91例,实验组施行健康促进,对照组只实行常规护理,24 w后采用生活质量量表(SF-36QOL)测定其生活质量.结果 实验组生活质量非常显著高于对照组(P＜0.001).结论 健康促进提高了产后抑郁症患者的生活质量.     

藏药八味沉香散50％醇提部位对急性心肌缺血大鼠作用的研究

目的：研究八味沉香散50％醇提部位对垂体后叶素诱发的急性心肌缺血大鼠的作用。方法：采用大鼠舌下静脉注射垂体后叶素复制急性心肌缺血模型,实验大鼠随机分为正常对照组、模型对照组、阳性对照组、八味沉香散50％醇提液大、小剂量组。观察八味沉香散50％醇提液对大鼠注射垂体后叶素后血清乳酸脱氢酶（LDH）、肌酸激酶（CK）和心肌超微结构的影响。结果：与模型对照组比较,八味沉香散50％醇提液预处理后可明显减少大鼠血CK和LDH的释放（均P〈0.01）,并减轻心肌超微结构线粒体的损伤。结论：八味沉香散50％醇提液对垂体后叶素所致大鼠急性心肌缺血具有一定的保护作用,而抗心肌缺血的作用可能与其保护心肌线粒体结构,改善缺血心肌的能量代谢有关。     

Optimization of Supercritical Fluid Extraction and Rapid Resolution LC-MS/ESI Identification of Chromones from Saposhnikoviae Radix through Orthogonal Array Design

Objective To establish a rapid and effective supercritical fluid extraction(SFE) and rapid resolution liquid chromatography method coupled with diode-array detector(RRLC-DAD) to quantify the chromones in a species. Methods The effects of four parameters including ethanol concentration(50%-90%), pressure(25-45 MPa), temperature(40-60 ℃), and time(30-90 min) on the chromones yields, namely prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, and sec-O-glucosylhamaudol, were investigated using SFE system with orthogonal array design(OAD). Furthermore, the extracts were analyzed using rapid resolution liquid chromatography coupled with diode-array detector(RRLC-DAD) system to confirm the results. Results Under the optimized conditions, i.e., 35 MPa of pressure, 60 ℃ of temperature, 70% ethanol, and 60 min of time, the yields of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, and total chromones were 3.514, 0.132, 6.242, 0.342, and 10.231 mg/g, respectively. In comparison with ultrasonic assisted extraction(UAE), SFE was able to yield a 20.7% increase in the total chromones from Saposhnikoviae Radix. Conclusion SFE is an alternative and promising method to extract chromones from this species, and the established RRLC-DAD method could serve as a rapid and effective method for the identification of chromones from Saposhnikoviae Radix.     

紫杉醇联合5-氟脲嘧啶持续输注治疗晚期胃癌的临床研究

目的 观察紫杉醇（PTX）联合5-氟脲嘧啶（5-Fu)持续静脉输注治疗晚期胃癌的疗效和毒性。方法 对30例晚期胃癌患者第1，第8，第15天使用PTX60mg/m^2，静脉滴注；第1-15天使用5-Fu0.25g/m^2,24h中心静脉持续输注。28天为1个周期，2个周期后评定疗效。平均3.43个周期。结果 28例可评价疗效。28例中完全缓解3例。部分缓解14例，总有效率为60.7%，中位缓解期为6.4个月。中位生存期为11.5个月。毒副作用主要为剂量限制性毒性，表现为骨髓抑制。结论 紫杉醇加5-氟脲嘧啶持续静脉输注是治疗晚期胃癌较好的化疗方案。     

A reproductive screening test of hawthorn

AIM OF STUDY: Hawthorn (Crataegus) has a long history as a medicine. The current clinical use of hawthorn as a heart medicine dates back to the late 19th century. It is well tolerated clinically yet contraindicated in pregnancy. MATERIALS AND METHODS: To determine the safety of hawthorn to the developing fetus, pregnant rats were dosed daily by gavage using 56 times the human dose of hawthorn on either gestation days (GD) 1-8 or GD 8-15. On GD 20, fetuses were weighed and examined for signs of external, internal or skeletal malformations. Rat fetuses were also explanted on GD 10.5 and cultured with hawthorn extract for 26 h. RESULTS: Hawthorn did not have an adverse effect on embryonic development in vivo or in vitro. CONCLUSIONS: While the results suggest that hawthorn, taken at the recommended dose would have no adverse effects on embryonic development this may be due to the low bioavailability of some hawthorn constituents when taken orally. Pharmacokinetic studies are required to determine the extent of absorption of hawthorn from the small intestine in healthy adults in order to verify its safety.     

全国胎儿医学与产前诊断学术研讨会纪要

由中华医学会<中华妇产科杂志>编委会主办、南方医科大学南方医院协办的全国胎儿医学与产前诊断学术研讨会于2007年9月14-17日在花城广州召开.<中华妇产科杂志>总编辑郎景和教授、副总编辑黄醒华教授和林其德教授,以及来自全国各地近300名产科、新生儿科等专家和代表参加了会议.会议系统介绍了从早孕到分娩期多种现代产科胎儿疾病诊断、治疗和胎儿监测技术,围绕胎儿医学、遗传咨询、宫内诊断等临床热点进行了热烈讨论.会议特邀澳大利亚南澳洲大学副校长McMillen教授、中国香港中文大学刘子建教授、首都医科大学附属北京妇产医院黄醒华教授等17位国内外著名专家作了专题讲座,并先后有13位代表进行了大会论文报告.现将会议内容纪要如下.