Signalling lymphocyte activation molecule family member 9 is found on select subsets of antigen-presenting cells and promotes resistance to Salmonella infection

Signalling lymphocyte activation molecule family member 9 (SLAMF9) is an orphan receptor of the CD2/SLAM family of leucocyte surface proteins. Examination of SLAMF9 expression and function indicates that SLAMF9 promotes inflammation by specialized subsets of antigen-presenting cells. Within healthy liver and circulating mouse peripheral blood mononuclear cells, SLAMF9 is expressed on CD11b⁺, Ly6C⁻, CD11c^low, F4/80^low, MHC-II⁺, CX₃CR1⁺ mononuclear phagocytes as well as plasmacytoid dendritic cells. In addition, SLAMF9 can be found on peritoneal B1 cells and small (F4/80^low), but not large (F4/80^high), peritoneal macrophages. Upon systemic challenge with Salmonella enterica Typhimurium, Slamf9^−/− mice were impaired in their ability to clear the infection from the liver. In humans, SLAMF9 is up-regulated upon differentiation of monocytes into macrophages, and lipopolysaccharide stimulation of PMA-differentiated, SLAMF9 knockdown THP-1 cells showed an essential role of SLAMF9 in production of granulocyte–macrophage colony-stimulating factor, tumour necrosis factor-α, and interleukin-1β. Taken together, these data implicate SLAMF9 in the initiation of inflammation and clearance of bacterial infection.     

Receptor-mediated calcium signalling in glial cells from mouse corpus callosum slices

We addressed the question of whether glial cells in intact white matter tracts express neurotransmitter receptors and we used Ca⁺⁺ signalling as a probe to detect the receptor activation. Corpus callosum slices from postnatal mice were bulk-loaded with the Ca⁺⁺- sensitive fluorescent dye fluo-3, and confocal microscopy was used to measure Ca⁺⁺ transients in response to neuroligands. Glial cell bodies were intensely dye-loaded and could be discriminated from the diffuse fluorescence of axons. Subpopulations of glial cells from slices obtained at postnatal days 3 to 7 responded with Ca⁺⁺ signals to ATP, glutamate, histamine, GABA, norepinephrine, serotonin, angiotensin II, bradykinin, and substance P. These subpopulations showed a distinct overlap; cells which were responsive to substance P always showed Ca⁺⁺ signalling in response to histamine, ATP, GABA, and high K⁺ (membrane depolarization). GABA-responsive cells almost always showed a [Ca⁺⁺], increase after membrane depolarization. In brain slices from postnatal day 11 to 18 animals, the Ca⁺⁺ responses were evident for glutamate, ATP, and norepinephrine, while GABA, substance P, serotonin, histamine, or angiotensin II rarely elicited a response. This study demonstrates that white matter glial cells in slices exhibit a large repertoire of neurotransmitter responses linked to Ca⁺⁺ signalling and that these receptor systems are differentially distributed on sub-populations of glial cells. © 1996 Wiley-Liss, Inc.     

Actor–observer asymmetry in perceptions of parole board release decisions

In the current study, the actor–observer effect is tested with both mock parole board members and the public evaluating the responsibility of parole board members for a decision resulting in a parolee reoffending and committing a murder. Participants (two samples with a combined N = 1317) were randomly assigned to act as a mock parole board member and make a decision (which ended in the parolee reoffending) or as a member of the public who read a story about the same parole decision and outcome. Findings suggest that the traditional actor–observer asymmetry emerged across blame and responsibility concepts, emotion and moral judgments. Overall, the public held harsher judgments than the mock parole board members. Implications regarding self-enhancement, methodology and attribution theory are discussed.Key words: actor–observer, attributions, blame, decision-making, emotion, parole, responsibility

Parole has been a part of corrections reform since the early twentieth century, and it shares many goals with a more rehabilitative criminal justice approach seen gaining momentum recently (Cullen, 2017; Petersilia, 2000). Perceptions that prison sentences were too harsh and did not allow any room for rehabilitation spurred support for a more lenient approach to parole, and models of releasing less violent inmates that have shown good behavior were adopted (Clear & Cole, 1997). In general, there are two types of parole decisions; discretionary and mandatory parole (Maruschak & Bonczar, 2013). Discretionary parole involves a decision process through which parole board members review an inmate’s files and decide whether to grant or deny supervised release (parole). There is no required action given to the board, but the action is left up to the discretion of the board. In contrast, mandatory parole is a decision process by which inmates are released under specific circumstances after they have served a certain period of time in prison and do not have major violations or other factors barring their release (Hughes et al., 2001). Under mandatory release guidelines, the parole board is often obligated to release inmates to supervised release, usually based on determinate sentencing and good behavior, unless some major factor prevents such action (Abadinsky, 2012). Recently, discretionary parole has been used at a higher rate than mandatory parole, creating a trend over the past several years (Kaeble, 2018; Maruschak & Bonczar, 2013).In the United States, there are currently about 875,000 inmates on parole, with over 450,000 inmates being released on parole in 2016 (Kaeble, 2018). With an increase in discretionary parole decisions and a large parole population, the parole board likely faces scrutiny for the outcomes of its decisions and is held accountable by the public for ensuring safety and economy (e.g. Abadinsky, 2012; Mackenzie, 2001; National Parole Resource Center, 2012; Paparozzi & Guy, 2009). Parole board members’ perspectives and the public’s perspective might differ as they relate to how parole decisions are perceived and the accountability of the boards’ decisions. This divergence is important because legislation applicable to parole board decisions is intended to incorporate both perspectives, and any dialogue about parole should involve an attempt at mutual understanding. However, it is possible that the divergence in perspectives is due to experience and observational standpoint. The purpose of the current study is to apply an attribution framework to better understand the difference between perspectives of actors (parole board members) and observers (the public) by using a mock parole decision-making paradigm and comparing both parole board and public perspectives on the decision process and outcome with a focus on blame and responsibility for negative outcomes.     

Epidemiology of non-traumatic spinal cord injury in Ireland – a prospective population-based study

Objective: To carry out a study of non-traumatic spinal cord injury (NTSCI) epidemiology in IrelandDesign: Prospective study of all new incident cases of NTSCI during 2017Setting: Republic of IrelandParticipants: All persons with a newly acquired NTSCIInterventions: NoneOutcome measures: Crude and age/sex specific incidences; ISCoS core dataset and non-traumatic dataset; population denominator was 2016 national census figures, adjusted to 2017.Results: Overall crude incidence of NTSCI in the Republic of Ireland in 2017 was 26.9 per million per year. Mean age at onset was 56.6 (SD 17.7) years. Females accounted for 51.2% of cases. Most frequent grade of ASIA impairment scale (AIS) was AIS D. Most common etiology was degenerate conditions (48.8%) followed by neoplastic (26.4%). The most common pattern of onset (51.2%) was lengthy (greater than one month).Conclusions: Incidence of NTSCI is more than double that for traumatic SCI in the Republic of Ireland. This suggests that the delivery of rehabilitation services to patients with spinal cord injuries requires prompt review and expansion.