库存软骨异体移植的动物实验研究──组织学、组织化学、电镜观察

为寻找理想的软骨库存液，本实验采用异体猪肋软骨皮下移植模型，研究了经０．４％戊二醛、甲醛混合液保存４周的软骨移植物在异体移植１、３、５月后的组织、组化及电镜观的变化。结果表明该保存液处理的软骨组织的细胞结构、酶化学生成物于移植５月后与正常组织相比变化不大。提示软骨细胞仍具有一定活力及增殖潜力。本实验认为０．４％戊二醛、甲醛混合液是一种优良的软骨库存液。     

创伤性面瘫物理疗法的临床研究

目的 比较超短波加离子导入疗法（A组）与电刺激加离子导入疗法（B组）治疗创伤性面瘫的效果。方法 对39例创伤性面神经损伤患者进行临床对照研究，通过量化面神经功能评价、神经电图检查及面神经功能的主观评价比较面神经损伤的治疗效果。结果 创伤性面瘫以青年男性多见，损伤程度以中、重度为主；对于神经损伤轻、中度患者,A组与B组均能促进神经恢复，B组神经功能开始恢复时间早于A组。对于神经损伤重度患者，A组与B组神经恢复率高于对照组，两组均不能减少并发症出现率。结论 超短波加离子导入疗法与电刺激加离子导入疗法均能促进面神经损伤的恢复，但对于减少并发症发生率并无帮助。     

PTEN、p27在口腔粘膜白斑和口腔癌组织中的表达及意义

目的　探讨抑癌基因PTEN、p2 7在口腔粘膜白斑 (oralleukoplakia ,OLK)和口腔癌 (oralsquamouscellcancer,OSCC)组织中的蛋白表达及意义。方法　应用免疫组化S -P法检测 10例正常口腔粘膜、2 5例口腔粘膜白斑 (其中白斑伴上皮异常增生 15例 ,白斑不伴上皮异常增生 10例 )及 32例口腔癌组织中抑癌基因PTEN和p2 7的蛋白表达情况。结果　正常口腔粘膜和白斑不伴上皮异常增生组织中PTEN和 p2 7蛋白全部阳性表达 ;白斑伴上皮异常增生组织中PTEN和 p2 7蛋白阳性表达率分别为93.3%、73.3%。OSCC组织中PTEN蛋白阳性表达率为71.9% ,其中高、中、低分化OSCC组织中PTEN蛋白阳性表达率分别为 85 .7%、75 %和 33.3% ,统计学分析表明PTEN在OSCC组织中的蛋白表达与组织分化程度明显相关 (P <0 .0 5 )。OSCC组织中 p2 7蛋白的阳性表达率为4 0 .6 % ,其中高、中、低分化OSCC组织中p2 7蛋白的阳性表达率分别为 4 2 .9%、4 1.7%和 33.3% ,统计学分析表明p2 7在OSCC组织中的蛋白表达与组织分化程度无明显相关 (P >0 .0 5 )。结论　OSCC组织中PTEN和 p2 7蛋白的阴性表达率较高 ,说明PTEN和 p2 7基因突变或缺失在OSCC的发生发展过程中起重要作用。     

Metastases to lingual lymph nodes from squamous cell carcinoma of the tongue

The diagnosis of squamous cell carcinoma (SCC) of the tongue metastasising to the lingual lymph nodes is rare. Until now lingual lymph nodes have not been mentioned in any system of lymph node classification. We report a case of SCC of the tongue with metastases to the lymph nodes in the superficial floor of the mouth, and to levels I and II lymph nodes. We provide evidence of the existence of lingual lymph nodes, and validate the practice of removing the lymph node-bearing-tissue of the floor of the mouth during neck dissection in cancers of the oral cavity.     

Measuring clinical attachment: reproducibility of relative measurements with an electronic probe.

During the past few years, improvements in detecting longitudinal changes in clinical attachment or alveolar bone density have been introduced. For example, constant-force electronic probes and computer-assisted subtraction analysis of longitudinal radiographs have been reported to be more sensitive and reproducible in detecting changes in clinical attachment or alveolar bone density, respectively. Use of these new technologies requires that sources of measurement error be identified and their contribution quantified. This study investigated the reproducibility of a constant-force electronic probe in a careful clinical setting. Measurements were performed from individually fabricated acrylic stents. Three groups of subjects were selected for the study: 10 adults without oral disease, 10 adults with gingivitis, and 10 adults with moderate periodontitis. Four probing designs were employed to investigate the reproducibility of the probe. In design A, the probe tip was left in the sulcus between successive probings. In design B, the probe tip was removed from the gingival margin between probings but the next probing followed immediately. In design C, successive whole-mouth probings were interrupted by a 5-minute interval and a mouth rinse. In design D there was a 4-week interval between each successive probing. Three measurements were taken at each site for each design. The main purpose of this study was to identify variance components in the attachment level variation. The maximum probing error standard deviation ranged from 0.2 to 0.3 mm depending on the periodontal health of the subject. This level of variation is considerably smaller than that found in most previous studies.(ABSTRACT TRUNCATED AT 250 WORDS)     

功能矫治器矫治安氏Ⅱ类Ⅰ分类错He的软组织侧貌变化

目的 用5项软组织美学指标，评价功能矫治器矫治安氏类Ⅰ分类错殆的软组织变化。方法 对21例安氏类Ⅰ分类错He患者用功能矫治器(FR-2)矫治前后的X线头影测量片进行分析，对比治疗前后软组织5项美学指标的变化。结果 通过早期的功能矫形治疗，患者大多数美学指标接近正常值范围，但也有部分指标改善不明显。结论 功能矫治能使大多数患者软组织侧貌得到明显改善，但存在个体差异，部分病例仍需要二期治疗。     

应用植入控释系统进行一次法根管治疗术的动物实验研究

目的：利用自制含氯霉素、甲硝唑等药物的控释系统，对实验家兔的血药浓度、药物抗菌活性和家兔根管、尖周组织的病理观察，为今后临床应用提供理论和实验依据。方法：应用组织病理观察和液相色谱分析技术。结果：用ＨＰＬＣ法得出实验用家兔血药浓度和抗菌活性均为阴性，根管及尖周组织病理切片无特殊表现。结论：自制控释系统是一种无毒、无副作用、有效的根管治疗药物剂型，可进行临床应用。     

正畸用粘接剂底涂剂的研制

目的 开发能与牙釉质产生较强粘接力，提高托槽在牙面上的粘接强度。提供正畸治疗理想的底涂剂。方法 引入不同活性稀释单体组成不同的底涂剂配方，进行粘接抗剪切强度测试。结果 筛选出以极性适中的HAMA组成的底涂剂，其性能较同类产品更为杰出，粘接抗剪切强度达23.6MPa。结论 在传统底涂剂配方中引入极性适中，液体表面张力适当的HAMA所形成的PR-2配方是本研究的理想的底涂剂配方。