全文获取类型
收费全文 | 3828篇 |
免费 | 83篇 |
国内免费 | 13篇 |
专业分类
耳鼻咽喉 | 10篇 |
儿科学 | 145篇 |
妇产科学 | 143篇 |
基础医学 | 231篇 |
口腔科学 | 106篇 |
临床医学 | 751篇 |
内科学 | 894篇 |
皮肤病学 | 216篇 |
神经病学 | 122篇 |
特种医学 | 9篇 |
外科学 | 451篇 |
综合类 | 27篇 |
一般理论 | 29篇 |
预防医学 | 254篇 |
眼科学 | 68篇 |
药学 | 392篇 |
肿瘤学 | 76篇 |
出版年
2016年 | 29篇 |
2015年 | 37篇 |
2014年 | 37篇 |
2013年 | 78篇 |
2012年 | 27篇 |
2010年 | 95篇 |
2009年 | 110篇 |
2008年 | 35篇 |
2006年 | 27篇 |
2005年 | 29篇 |
2003年 | 26篇 |
2001年 | 47篇 |
1999年 | 90篇 |
1998年 | 152篇 |
1997年 | 192篇 |
1996年 | 190篇 |
1995年 | 128篇 |
1994年 | 163篇 |
1993年 | 119篇 |
1992年 | 118篇 |
1991年 | 111篇 |
1990年 | 99篇 |
1989年 | 114篇 |
1988年 | 118篇 |
1987年 | 97篇 |
1986年 | 96篇 |
1985年 | 87篇 |
1984年 | 86篇 |
1983年 | 95篇 |
1982年 | 83篇 |
1981年 | 77篇 |
1980年 | 70篇 |
1979年 | 90篇 |
1978年 | 76篇 |
1977年 | 41篇 |
1976年 | 40篇 |
1975年 | 39篇 |
1974年 | 39篇 |
1973年 | 25篇 |
1971年 | 41篇 |
1970年 | 32篇 |
1969年 | 28篇 |
1968年 | 43篇 |
1967年 | 34篇 |
1966年 | 30篇 |
1965年 | 33篇 |
1964年 | 25篇 |
1963年 | 30篇 |
1962年 | 32篇 |
1957年 | 25篇 |
排序方式: 共有3924条查询结果,搜索用时 15 毫秒
51.
BEERENDONK GEERTJE J. M. VAN; PEARSON PAUL G.; MEIJER DIRK K. F.; MULDER GERARD J.; NELSON SIDNEY D.; MEERMAN JOHN H. N. 《Toxicological sciences》1995,28(1):111-117
The metabolism of tris(2,3-dibromopropyl) phosphate (Tris-BP)was compared with that of completely deuterated Tris-BP (D15-Tris-BP)in an isolated, recirculating rat liver perfusion system inorder to determine the relative quantitative importance of twodifferent biotransformation pathways of Tris-BP: (i) cytochromeP450-mediated metabolism and (ii) GSH S-transferase-mediatedmetabolism. To accomplish this we quantitated the biliary excretionof S-(3-hydroxypropyl)glutathione (GSOH) as a marker metabolitefor cytochrome P450-mediated metabolism and that of S-(2,3-dihydroxypropyl)glutathione (GSOHOH) as a marker metabolite for GSH S-transferase-mediatedmetabolism. Completedeuterium substitution of Tris-BP significantlydecreased the formation of GSOH, whereas there was no effecton the formation of GSOHOH. Because our previous studies showeda large decrease in genotoxicity of D15-Tris-BP compared toTris-BP, the present results support our hypothesis that cytochromeP450-mediated metabolism is responsible for the genotoxic effectsof Tris BP in the rat liver. 相似文献
52.
SZAREK JOHN L.; STEWART NANCY L.; ZHANG JASON Z.; WEBB JEFFREY A.; VALENTOVIC MONICA A.; CATALANO PAUL 《Toxicological sciences》1995,28(2):199-208
Short-term exposure to high concentrations of ozone has beenshown to increase airway responsiveness in normal humans andin all laboratory animal species studied to date. While ourknowledge concerning the pulmonary effects of single exposuresto ozone has increased rapidly over recent years, the effectsof repeated exposures are less understood. The goal of the presentstudy was to determine whether airway responsiveness is increasedafter near-lifetime exposure to ozone. Airway segments representingapproximately eighth generation airways were isolated from Fischer344 rats of both genders that had been exposed for 6 hr perday, 5 days per week for 20 months to 0, 0.12, 0.5, or 1.0 partsper million (ppm) ozone. Circtimferential tension developmentwas measured in isolated airways in response to bethanechol,acetylcholine, and electrical field stimulation. Responsivenessof the airways to the contractile stimuli was described by theeffective dose or frequency that elicited half-maximum contraction(ED50) and the maximum response. Since ozone exposure is associatedwith remodeling of peripheral airways, smooth muscle area wasdetermined and tension responses were normalized to the areameasurements. Before normalization of tension data to smoothmuscle area, neither the ED50 nor maximum response of smallbronchi to the contractile stimuli was altered after chronicozone exposure. Smooth muscle area was greater in airways isolatedfrom animals that had been exposed to 0.5 ppm ozone. After accountingfor smooth muscle area, maximum responses of the small bronchiisolated from male rats were significantly reduced after 0.12and 0.5 ppm ozone. Although not significant statistically, asimilar trend was observed in airways isolated from female rats.These results suggest that the increase in airway responsivenessassociated with acute ozone exposure does not persist duringnear-lifetime exposure. Although the mechanism responsible forthe adaptation to the effects of 03 on airway responsivenessis unknown, the results indicate that smooth muscle cell functionwas compromised by the chronic exposure. The mechanism(s) responsiblefor mediating this effect and the relevance of these resultsto humans remains to be determined. 相似文献
53.
CLIVE DONALD; TURNER NANCY T.; HOZIER JOHN; BATSON A. GAIL; TUCKER WALTER E. Jr. 《Toxicological sciences》1983,3(6):587-602
Preclinical Toxicology Studies with Acyclovir: Genetic ToxicityTests. Clive, D., Turner, N.T., Hozier, J., Batson, A.G. andTucker, W.E., Jr. (1983). Fundam. Appl. Toxicol 3: 587602.Acyclovir (ACV), an antiviral drug active in the treatment oforal and genital Herpes infections, has been evaluated for mutagenicand carcinogenic potential in a battery of in vitro and in vivoshort-termassays. Negative results were obtained in the following in vitrotests: Ames Salmonella, plate incorporation and preincubationmodification assays; E. coli polA+/polA DNA repair; yeast(S. cerevisiae D4) gene conversion; Chinese hamster ovary cells(HGPRT, APRT loci and ouabain-resistance marker); L5178 Y mouselymphoma cells (HGPRT locus and ouabain-resistance marker);and C3H/10Tmouse fibroblast neo-plastic transformation assay.All except the last assay were performed in the presence andabsence of an exogenous metabolic activation system. ACV waspositive at high concentrations x exposure times in the absenceof exogenous metabolic activation in the following in vitrosystems and at the indicated concentrations: BALB/c-3T3 neoplastictransformation (50 /µg/mL, 72 h exposure); human lymphocytecytogenetics (250500 µg/mL, 48 h exposure); andL5178Y mouse lymphoma cells (TK locus, 4002400 µg/mL,4 h exposure; predominantly small colony mutants of chromosomalorigin produced). No effects were seen in vivo (mouse dominantlethal assay; rat and Chinese hamster bone marrow cytogenetics)at up to maximum tolerated doses (MTD). An unusual clastogeniceffect was seen in Chinese hamsters at 5 times the MTD. Overall,positive effects were seen only at either high concentrations(250 µg/mL in vitro or plasma levels) or prolonged exposure(72 hr in the BALB/ c-3T3 neoplastic transformation assay).These studies support the view that ACV is a chromosomal mutagen,i.e., one which causes multi-locus damage but not single geneeffects. The significance of these results for the genetic riskof ACV to man is discussed. 相似文献
54.
SLIKKER WILLIAM JR; PAULE MERLE G.; ALI SYED F.; ANDREW C. SCALLET; BAILEY JOHN R. 《Toxicological sciences》1991,17(2):321-334
Chronic Marijuana Smoke Exposure in the Rhesus Monkey I. PlasmaCannabinoid and Blood Carbxyhemoglobin Concentrations and ClinicalChemistry Parameters SLIKKER, W., JR., PAULE, M. G., ALI, S.F., SCALLET, A. C., AND BAILEY, J. R (1991). Fundam. Appl Toxicol17, 321334. This report is the first in a series abouta large multidisciplinary study designed to determine whetherchronic marijuana (MJ) smoke exposure results in residual behavioraland/or neuropathological alterations in the rhesus monkey. Priorto the initiation of a year of chronic MJ smoke exposure, 64periadolescent male rhesus monkeys were trained for 1 year toperform five operant behavioral tasks and then divided, accordingto their performance in these tasks, into four exposure groups(n=1516/group): (1) a high dose (HI) group, exposed 7days/week to the smoke of one standard MJ cigarette; (2) a lowd m (LO) group, exposed on weekend days only to the smoke ofa standard MJ cigarate; (3) an extracted MJ cigarette (EX) group,exposed 7 days/week to the smoke of one ethanol-extracted MJcigarette; and (4) a sham group (SH), exposed 7 days/week tosham exposure conditions. Daily exposures for 1 year were accomplishedusing a mask that covered the subjects' nose and mouth. Averagebody weights (initially 3.7?0.5 kg, mean?SD) and rates of weightgain (approximately 0.1 kg/month) were the same for all groupsthroughout the entire experiment. During the first week of expsure,plasma concentrations of -9-tetrahydrocannabinol and 11-nor-9-carboxy-THCin the HI group were 59?7 (mean?SE) and 5.5?1.5 ng/ml, respectively,45 min after MJ smoke administration and did not change significantlyat similar times after exposure throughout the remainder ofthe year. Whole blood carboxyhemoglobin levels increased toapproximately 13% 1 min after expsure to smoke in either theMJ or the EX groups. Comparison of blood chemistry and hematologyvalues before, during, and after exposure indicated no differencesfor most parameters. During exposure, lymphocytes, alkalinephosphatase and -glutamyl transferase were depressed in theHI group compared to in the SH group. During exposure, aspartateaminotransferase was elevatd for both the HI and EX groups,suggesting a general effect of smoke exposure. Because theseeffects were transient and remained within the range of reportednormal values, these data indicate that long-term, experimentalexperimental exposure to MJ smoke is feasible and does not compromisethe general health of the rhesus monkey. 相似文献
55.
KATSUTANI NARUO; YOSHITAKE SHINJI; TAKEUCHI HITOSHI; KELLIHER JOHN C.; COUCH RONALD C.; SHIONOYA HIROSHI 《Toxicological sciences》1992,19(4):555-562
Immunogenic properties of second generation human tissue plasminogenactivator (tPA) derivatives were examined in chimpanzee andmouse systems. Five species of modified tPAs (mtPAs) (designated2660, 2663, 2810, 8000, and 9200), recombinant native tPA orbovine serum albumin (BSA) as a positive control were subcutaneouslyinjected nine times at suitable intervals into chimpanzees,genetically the closest species to man. These animals were testedfor antigen(Ag)-specific antibodies to the corresponding proteinsby means of enzyme-linked immunosorbent assay and Western blotanalysis. Neither 9200, one of the five mtPAs tested, nor tPAwas immunogenic, although BSA and the other four mtPAs wereimmunogenic under these conditions. Thus, an antigenic determinant was not exposed by the modification on 9200 and thismodified tPA is expected not to be immunogenic in humans. Inthe mouse studies, mice were immunized with mtPAs. Serum sam-piesfrom these animals were tested for antibodies to the mtPAs whichdid not concomitantly recognize native tPA by immune ad sorptionof the antibodies to tPA. The amount of such antibodies alterthe elimination of native tPA-reactive antibodies was littleor none when the serum samples from 9200 and from the othermtPAs, except 8000, were tested. Taking into consideration theresults of the chimpanzee studies, it can be concluded thatAg-specific antibodies are dominantly produced to unchangedepitopes present in modified proteins in the mouse system, inwhich the native protein is immunogenic. These results suggestthat the chimpanzee model should be useful to predict immunogenicityof second generation recombinant proteins in man, while themouse system adopted by us, which determines the newly generatedepitopes of the modified proteins, is not sufficient. 相似文献
56.
MAYA BUTTERFIELD M. Sc. JOHN FISHER Ph .D. JOHN NIGEL KEARNEY Ph .D.† GWILYM ALBAN DAVIES F.R.C.S. 《Journal of cardiac surgery》1991,6(4):490-498
The hydrodynamic function and leaflet dynamics of second generation porcine valves prepared with low- or zero-pressure fixation have been studied and compared to first generation porcine bioprostheses, bileaflet, and tilting disc mechanical valves. The Carpentier-Edwards Supra-Annular and Hancock II valves showed lower pressure drops than the Medtronic Intact valve and first generation porcine valves, and comparable overall energy losses to mechanical valves at normal cardiac outputs. Only the zero-pressure fixed Intact valve showed synchronous leaflet opening. Delayed leaflet opening and high opening pressures were found in both low- and high-pressure fixed porcine valves. All porcine bioprostheses showed high open leaflet bending strains. Fixation of valve leaflets with "near zero" pressure fixation and a more physiological neutral geometry is necessary to ensure synchronous leaflet opening at low flows and a reduction in commissural bending strains. 相似文献
57.
Ventilation (VI), end-tidal (Pco2), mixed venous (PVco2) andthe ventilatory response to carbon dioxide were measured beforesurgery, and during the first 4h of recovery in 18 adult patientswho underwent elective limb surgery under fentanyl or Innovaranaesthesia. End-tidal and mixed venous PCO2 were increasedsignificantly in the first 150 min after the last dose of drug(P<0.001, P<0.01). but had returned to control valuesby 4 h. Ventilation and ventilatory response to carbon dioxidewere significantly depressed in all patients (P < 0.001),but returned to near control values at 4 h. Fentanyl and Innovaranaesthesia displaced the carbon dioxide response to the right,but no correlation was found between either the magnitude ofthe displacement of the response curve or the alteration inslope and the control values. This suggests that patients witha low value of VI/Pco2 are not more susceptible to the ventilatorydepressant action of narcotic anaesthetics. Recovery of ventilatoryresponsiveness towards normal during the 4 h after anaesthesia,occurred in a graded and progressive manner; there was no evidenceof a biphasic pattern of recovery.
*Present address: Centre for Advanced Studies in Health Sciences,Western Australian Institute of Technology, Bentley, WesternAustralia.
Presented to the annual meeting of the Canadian AnaesthetistsSociety, Ottawa, Ontario, June 1978. 相似文献
58.
A quantitative study of the degree of racemization induced by the [(NH3)5Co-(III)-] protecting group when bound to the C-terminal of the amino acids Leu, Phe, and His, as has been carried out. Racemization was determined by forming the diastereomeric cobalt dipeptides [(Leu)(AA)Co(III)(NH3)5] where AA = L-Leu, L-Phe, and L-His; after cobalt removal (using NaBH4), the peptide diastereomers were analyzed quantitatively using an amino acid analyzer. No racemization was observed within experimental error (0.3%) as a result of the substitution of the [(NH3)5Co(III)-] group on the amino acids and peptides studied. 相似文献
59.
60.
STUDIES IN NAIL GROWTH 总被引:2,自引:0,他引:2