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991.
HL Zaaijer ; GA van Rixel; JN Kromosoeto ; DR Balgobind-Ramdas ; HT Cuypers ; PN Lelie 《Transfusion》1998,38(8):776-781
BACKGROUND: Used as a supplemental assay, new anti-human immunodeficiency virus (HIV) immunoblots, employing recombinant and synthetic antigens, appeared to resolve the majority of samples with false-reactive Western blot results. Would it be possible to completely replace the Western blot by an immunoblot for confirmation and exclusion of HIV infection? STUDY DESIGN AND METHODS: The sensitivity of the new LiaTek HIV III immunoblot assay (Organon Teknika, Turnhout, Belgium) was tested on 416 Western-blot positive samples (386 HIV-1, 22 HIV-2, 1 HIV-1/2, and 7 HIV-O) and on 45 HIV-1 seroconversion samples. The specificity was tested on 146 samples from noninfected donors with false-positive results on a HIV screening test. RESULTS: All Western- blot-positive samples tested positive in the immunoblot (sensitivity: 100%). The immunoblot could not discriminate between HIV-1 and HIV-2 infection in 22 of 416 (5%) samples. The LiaTek assay showed reactivity in 28 of 45 seroconversion samples, whereas the Western blot reacted in 30 of 45 seroconversion samples. With false-positive donor samples, the immunoblot was indeterminate in 10 of 146 samples (specificity: 93%), and the Western blot was indeterminate in 44 of 146 samples (specificity: 70%). CONCLUSION: Like the Western blot, the immunoblot runs the risk of missing samples that are reactive by enzyme immunoassay during the early stage of HIV infection. Nevertheless, considering its superior specificity on false-positive donor samples, it appears that the immunoblot offers a cost-effective alternative to the Western blot assay for confirmation and exclusion of HIV infection. 相似文献
992.
993.
A Novel Imaging Marker for Small Vessel Disease Based on Skeletonization of White Matter Tracts and Diffusion Histograms
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Ebru Baykara MSc Benno Gesierich PhD Ruth Adam PhD Anil Man Tuladhar MD J. Matthijs Biesbroek MD PhD Huiberdina L. Koek MD PhD Stefan Ropele PhD Eric Jouvent MD PhD Alzheimer's Disease Neuroimaging Initiative Hugues Chabriat MD PhD Birgit Ertl‐Wagner MD Michael Ewers PhD Reinhold Schmidt MD Frank‐Erik de Leeuw MD PhD Geert Jan Biessels MD PhD Martin Dichgans MD Marco Duering MD 《Annals of neurology》2016,80(4):581-592
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995.
Emily Ronez Carole Geara Sylvie Coito Hugues Jacqmin Edouard Cornet Xavier Troussard 《Scandinavian journal of clinical and laboratory investigation》2017,77(6):406-409
Neutropenia is one of the main criteria for a blood smear review. The objective of this study was to compare the thresholds proposed by the international consensus group for hematology review (1.0 109/L) and the French speaking Group for Cellular Haematology (1.5 109/L) in terms of the number of useless smears. We collected 112,097 analyzed samples from four laboratories equipped with XN instruments (Sysmex, Kobe, Japan) during early 2016. The only exclusion criterion was a leucocyte count below 0.5 109/L. In the absence of abnormal cells and/or morphology suggesting haematological disease, samples were classified as ‘negative for morphology’ and the differential from the XN-10 was reported. These smear procedures were considered as uninformative. Some 2202 samples met the criterion for neutropenia (<1.5 109/L) for slide review representing 1.96% of the total. These included 1031 with neutropenia alone and 1171 neutropenia plus other abnormalities. Of the 1031 with neutropenia alone, 886 had a neutrophil count between 1.0 109/L and 1.5 109/L. The smear was uninformative for all of these samples. In conclusion, microscopic examination of a blood smear provided very limited information in cases of neutropenia without other abnormalities. 相似文献
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998.
Di Carlo Davide Tiziano Cagnazzo Federico Anania Yury Duffau Hugues Benedetto Nicola Morganti Riccardo Perrini Paolo 《Neurosurgical review》2020,43(3):987-997
Neurosurgical Review - The surgical resection of insular gliomas remains a challenge. Middle cerebral artery perforating arteries and deep functional pathways affect the extent of resection and the... 相似文献
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Genetic divergence of human immunodeficiency virus type 1 Ethiopian clade C reverse transcriptase (RT) and rapid development of resistance against nonnucleoside inhibitors of RT
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