全文获取类型
收费全文 | 332篇 |
免费 | 29篇 |
国内免费 | 34篇 |
专业分类
耳鼻咽喉 | 19篇 |
儿科学 | 11篇 |
妇产科学 | 2篇 |
基础医学 | 53篇 |
口腔科学 | 2篇 |
临床医学 | 46篇 |
内科学 | 53篇 |
皮肤病学 | 2篇 |
神经病学 | 33篇 |
特种医学 | 36篇 |
外科学 | 32篇 |
综合类 | 14篇 |
预防医学 | 44篇 |
药学 | 39篇 |
中国医学 | 1篇 |
肿瘤学 | 8篇 |
出版年
2021年 | 5篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 6篇 |
2017年 | 7篇 |
2016年 | 5篇 |
2015年 | 4篇 |
2014年 | 8篇 |
2013年 | 22篇 |
2012年 | 6篇 |
2011年 | 5篇 |
2010年 | 12篇 |
2009年 | 12篇 |
2008年 | 7篇 |
2007年 | 23篇 |
2006年 | 7篇 |
2005年 | 13篇 |
2004年 | 9篇 |
2003年 | 9篇 |
2002年 | 10篇 |
2001年 | 10篇 |
2000年 | 12篇 |
1999年 | 5篇 |
1998年 | 11篇 |
1997年 | 16篇 |
1996年 | 10篇 |
1995年 | 18篇 |
1994年 | 12篇 |
1993年 | 9篇 |
1992年 | 7篇 |
1991年 | 4篇 |
1990年 | 10篇 |
1989年 | 4篇 |
1988年 | 16篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 9篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1970年 | 5篇 |
1969年 | 5篇 |
1968年 | 3篇 |
排序方式: 共有395条查询结果,搜索用时 453 毫秒
11.
Purinergic signalling: an experimental perspective 总被引:1,自引:0,他引:1
Investigation of the multiple roles of extracellular nucleotides in the cochlea has developed from analysis of ATP-activated conductances in single sensory hair cells. Molecular probes such as radiolabelled ATP analogues and radiolabelled mRNA for ATP-gated ion channel subunits (P2X receptors) rapidly revealed the extensive nature of ATP signalling in this sensory organ. This has provided a foundation for physiological investigations which put extracellular nucleotides at the centre of homeostatic regulation of the driving force for sound transduction, modulation of mechanical tuning, control of cochlear blood flow and auditory neurotransmission. The purinergic signal transduction pathways associated with these processes have several novel features of significance to the broader field of purinergic neuroscience. In turn, these studies have benefited from the recent experimental advances in the field of purinergic signalling, a significant component of which is associated with the work of Professor Geoffrey Burnstock. 相似文献
12.
13.
14.
Normal human platelets were shown to contain the enzyme dihydropteridine reductase. The enzyme was not found in a variety of other cells of hematogenous origin. Partial purification and kinetic and physical data indicated that the platelet enzyme is similar to that previously characterized from liver. Dihydropteridine reductase is important for the regeneration of tetrahydrobiopterin, a required cofactor in hydroxylation reactions involved in biogenic amine formation. The presence of the enzyme may indicate that some synthesis de novo of serotonin and/or catecholamines occurs in platelets, as opposed to a purely storage and transport function. In addition, screening for hyperphenylalaninemia due to dihydropteridine reductase deficiency may become feasible by assaying platelets for enzyme activity. 相似文献
15.
16.
J Hickson W F Housley C Boyle 《International journal of aging & human development》1988,26(3):191-199
The present study examined Rotter's Internal-External (I-E) locus of control (LOC) concept in relation to life satisfaction and death anxiety in an aged population. Age and sex of the individual were also considered. In the case of life satisfaction, a strong sex and a strong locus of control effect were found. For death anxiety, again a strong sex effect was found, but there was also a significant interaction between locus of control and age. The article suggests the need for a life span developmental perspective in LOC research. Studies which explore the influence of life experiences, situational and environmental variables, and their effect on control orientation are also needed. 相似文献
17.
Molecular characterization of the t(2;5) (p23; q35) translocation in anaplastic large cell lymphoma (Ki-1) and Hodgkin's disease 总被引:1,自引:3,他引:1
18.
Rashid Ghaznawi Maarten HT Zwartbol Nicolaas PA Zuithoff Jeroen de Bresser Jeroen Hendrikse Mirjam I Geerlings 《Journal of cerebral blood flow and metabolism》2021,41(6):1229
Global cerebral hypoperfusion may be involved in the aetiology of brain atrophy; however, long-term longitudinal studies on this relationship are lacking. We examined whether reduced cerebral blood flow was associated with greater progression of brain atrophy. Data of 1165 patients (61 ± 10 years) from the SMART-MR study, a prospective cohort study of patients with arterial disease, were used of whom 689 participated after 4 years and 297 again after 12 years. Attrition was substantial. Total brain volume and total cerebral blood flow were obtained from magnetic resonance imaging scans and expressed as brain parenchymal fraction (BPF) and parenchymal cerebral blood flow (pCBF). Mean decrease in BPF per year was 0.22% total intracranial volume (95% CI: –0.23 to –0.21). Mean decrease in pCBF per year was 0.24 ml/min per 100 ml brain volume (95% CI: –0.29 to –0.20). Using linear mixed models, lower pCBF at baseline was associated with a greater decrease in BPF over time (p = 0.01). Lower baseline BPF, however, was not associated with a greater decrease in pCBF (p = 0.43). These findings indicate that reduced cerebral blood flow is associated with greater progression of brain atrophy and provide further support for a role of cerebral blood flow in the process of neurodegeneration. 相似文献
19.
Hematopoietic growth factors not only modulate blood progenitor cell activity but also alter the function of mature phagocytes. Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF; 1 ng/mL for 60 min) did not stimulate luminol-enhanced chemiluminescence of polymorphonuclear leukocytes (PMNs) in suspension but primed PMN for as much as a 15-fold increase in chemiluminescence in response to f-met- leu-phe (fMLP). Mixed mononuclear leukocytes (monocytes [approximately 20%] and lymphocytes [approximately 80%]; MNL) chemiluminescence was very low even after rhGM-CSF priming, but MNLs added to the PMNs (PMN- MNL) resulted in near doubling of rhGM-CSF-primed PMN fMLP-stimulated chemiluminescence. The enhancing factor(s) from MNLs were inherent rather than induced by the GM-CSF, and purified lymphocytes increased GM-CSF-primed PMN chemiluminescence equal to mixed MNLs. We could not detect cell-free "enhancing factor(s)," but cell-to-cell contact further enhanced rhGM-CSF-primed fMLP-stimulated PMN-MNL oxidative activity by 40%. Polyclonal rabbit anti-tumor necrosis factor (TNF) (but not preimmune serum) decreased both fMLP-stimulated rhGM-CSF- primed PMNs and PMN-MNL chemiluminescence, suggesting that TNF on the PMN surface is enhancing GM-CSF-primed chemiluminescence. GM-CSF priming markedly increased PMN superoxide release (sevenfold), but PMN superoxide release was not further enhanced by the presence of MNLs. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) and interleukin-3 (rhIL-3) displayed much smaller effects on pure PMNs and mixed PMN-MNL chemiluminescence and superoxide release than rhGM-CSF. rhGM-CSF primes PMNs for increased oxidative activity more than rhG-CSF and rhIL-3. Maximal oxidative activity was observed when mixed PMN-MNL were primed with GM-CSF in a cell pellet-promoting cell-to-cell contact. This enhanced activity can be attributed, in part, to both inherent enhancing factor(s) on lymphocytes and PMN-associated TNF induced by GM-CSF. 相似文献
20.
Ann Chi Yan Wong Lutz Birnbaumer Gary D. Housley 《The European journal of neuroscience》2013,37(9):1478-1486
The physiological significance of canonical transient receptor potential (TRPC) ion channels in sensory systems is rapidly emerging. Heterologous expression studies show that TRPC3 is a significant Ca2+ entry pathway, with dual activation via G protein‐coupled receptor (GPCR)–phospholipase C–diacylglycerol second messenger signaling, and through negative feedback, whereby a fall in cytosolic Ca2+ releases Ca2+–calmodulin channel block. We hypothesised that the latter process contributes to cochlear hair cell cytosolic Ca2+ homeostasis. Confocal microfluorimetry with the Ca2+ indicator Fluo‐4 acetoxymethylester showed that, when cytosolic Ca2+ was depleted, Ca2+ re‐entry was significantly impaired in mature TRPC3?/? inner and outer hair cells. The impact of this disrupted Ca2+ homeostasis on sound transduction was assessed with the use of distortion product otoacoustic emissions (DPOAEs), which constitute a direct measure of the outer hair cell transduction that underlies hearing sensitivity and frequency selectivity. TRPC3?/? mice showed significantly stronger DPOAE (2f1 ? f2) growth functions than wild‐type (WT) littermates within the frequency range of best hearing acuity. This translated to hyperacusis (decreased threshold) measured by the auditory brainstem response (ABR). TRPC3?/? and WT mice did not differ in the levels of temporary and permanent threshold shift arising from noise exposure, indicating that potential GPCR signaling via TRPC3 is not pronounced. Overall, these data suggest that the Ca2+ set‐point in the hair cell, and hence membrane conductance, is modulated by TRPC3s through their function as a negative feedback‐regulated Ca2+ entry pathway. This TPRC3‐regulated Ca2+ homeostasis shapes the sound transduction input–output function and auditory neurotransmission. 相似文献