全文获取类型
收费全文 | 7954篇 |
免费 | 620篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 105篇 |
儿科学 | 250篇 |
妇产科学 | 306篇 |
基础医学 | 1016篇 |
口腔科学 | 145篇 |
临床医学 | 1003篇 |
内科学 | 1521篇 |
皮肤病学 | 101篇 |
神经病学 | 790篇 |
特种医学 | 186篇 |
外国民族医学 | 1篇 |
外科学 | 927篇 |
综合类 | 99篇 |
一般理论 | 12篇 |
预防医学 | 1015篇 |
眼科学 | 105篇 |
药学 | 500篇 |
中国医学 | 9篇 |
肿瘤学 | 502篇 |
出版年
2023年 | 63篇 |
2022年 | 104篇 |
2021年 | 206篇 |
2020年 | 102篇 |
2019年 | 218篇 |
2018年 | 210篇 |
2017年 | 145篇 |
2016年 | 149篇 |
2015年 | 199篇 |
2014年 | 254篇 |
2013年 | 429篇 |
2012年 | 544篇 |
2011年 | 544篇 |
2010年 | 277篇 |
2009年 | 249篇 |
2008年 | 499篇 |
2007年 | 455篇 |
2006年 | 464篇 |
2005年 | 453篇 |
2004年 | 400篇 |
2003年 | 340篇 |
2002年 | 330篇 |
2001年 | 148篇 |
2000年 | 149篇 |
1999年 | 110篇 |
1998年 | 68篇 |
1997年 | 61篇 |
1996年 | 64篇 |
1995年 | 60篇 |
1994年 | 60篇 |
1993年 | 42篇 |
1992年 | 96篇 |
1991年 | 98篇 |
1990年 | 75篇 |
1989年 | 95篇 |
1988年 | 83篇 |
1987年 | 62篇 |
1986年 | 49篇 |
1985年 | 57篇 |
1984年 | 50篇 |
1983年 | 42篇 |
1982年 | 33篇 |
1979年 | 46篇 |
1978年 | 31篇 |
1977年 | 26篇 |
1973年 | 25篇 |
1972年 | 29篇 |
1971年 | 29篇 |
1970年 | 25篇 |
1968年 | 28篇 |
排序方式: 共有8593条查询结果,搜索用时 15 毫秒
991.
Dr. Virginia J. Nash M.S. Dr. Thomas P. Johnston Ph.D. Dr. Warren K. Palmer Ph.D. 《Pharmacotherapy》1996,16(1):10-15
We attempted to determine the mechanism(s) of poloxamer (P)-407-induced hyperlipidemia in rats by administering a lipid-lowering drug with a known mechanism of action. Five weight-matched animals were assigned to each of four treatment groups. Two groups received P-407 300 mg/ml and two received saline 1 ml. One of the P-407 and one of the saline groups were administered nicotinic acid 100 mg/kg by intraperitoneal injection at 6–96 hours after blood sampling. Blood samples were collected at 7 points from time zero to 120 hours and analyzed for triglyceride and cholesterol concentrations. The detergent produces hypertriglyceridemia (HTG) increasing from 53.4 ± 7.0 mg/dl (time zero) to 4026.9 ± 42.1 mg/dl by 24 hours. The HTG response was significantly attenuated by nicotinic acid (at t = 24 hrs). This, however, was followed by an average triglyceride concentration increase of 2.8-fold from 72 to 120 hours. The detergent produces a dramatic hypercholesterolemia (HCHO), increasing cholesterol from 47.5 ± 1.8 mg/dl to 468.5 ± 27.9 mg/dl by 48 hours. The HCHO was significantly affected by nicotinic acid administration during the accumulation phase. Nicotinic acid reduced cholesterol concentration from 364.4 ± 16.1 mg/dl to 276.8 ± 16.4 mg/dl at 24 hours (p<0.05). It is a potent antilipolytic agent, limiting the free fatty acids available for the synthesis of triglyceride and cholesterol. These data suggest that P-407 may act by stimulating the release of free fatty acids from the adipocyte for at least 24 hours after injection. 相似文献
992.
A L Hamada T Maruo T Samoto S Yoshida H Nash I M Spitz E Johansson 《Gynecological endocrinology》2003,17(3):247-254
In order to investigate whether vaginal rings delivering estradiol and progesterone could prevent endometrial hyperplasia and relieve climacteric symptoms, two variants of rings were used in 20 postmenopausal women with intact uteri for 4 months. One ring designated as PI-002 (n = 8) delivered in vitro estradiol 160 microg/day and progesterone 20 mg/day, while the other (PI-003; n = 12) delivered the same dosage of estradiol but only half the progesterone (10 mg/day). Serum estrone, estradiol and progesterone were measured at pretreatment, weekly for 4 weeks, and then monthly for 4 months. The incidence of hot flushes, frequency of night sweats, mood scores, vaginal discharge and bleeding profiles were recorded. Endometrial thickness was monitored by ultrasonography. The mean estrone level was 50 pg/ml for 16 weeks. The mean serum estradiol level was 75 pg/ml for the first 4 weeks and gradually decreased to 50 pg/ml at 16 weeks. The mean progesterone level with the PI-002 ring was 5 ng/ml for the first 4 weeks and decreased to 3.5 ng/ml at 16 weeks. With the PI-003 ring, the mean progesterone level was initially 3.5 ng/ml and then decreased to 2.5 ng/ml thereafter. Significant decreases in the incidence of hot flushes and night sweats as well as a striking improvement in mood scores were noted as early as 2 weeks after insertion. Three of the 20 women discontinued the treatment, owing to ring expulsion. Increased vaginal discharge was observed with both rings in the first 6 weeks. Vaginal bleeding was more frequently apparent among users of the PI-002 ring, although bleeding and spotting were confined to the first 6 weeks. Ultrasonographic monitoring of the endometrium constantly revealed a thickness of < 3 mm for both variants throughout use for 16 weeks. An estradiol/progesterone-releasing vaginal ring is a potential alternative to long-term hormone replacement therapy with minimum attention required. It provides effective protection against endometrial hyperplasia. 相似文献
993.
994.
995.
Reversibly sickled cells from patients with homozygous sickle-cell disease were prepared by Percoll-Isopaque density gradient separation and subjected to 15 h of cyclical deoxygenation-reoxygenation in the presence of Ca. After 15 h the sickle cells became dehydrated, losing volume secondary to K efflux via the Ca-activated (Gardos) channel, and showed impaired filterability through 5 microns diameter pores. The substituted benzaldehydes 12C79 and 589C80, which stabilize the oxy-conformation of sickle haemoglobin, showed an additional protective effect at pharmacological concentration by maintaining the K concentration, mean cell volume, and deformability of sickle cells. Drugs that increase the oxygen affinity of sickle haemoglobin may be more effective than specific inhibitors of Ca entry or K efflux in preserving the cation homeostasis and deformability of sickle cells during sickling in vivo. 相似文献
996.
997.
Twenty-nine of 34 (85%) Zn-finger-active compounds at 300 μM or less inhibited the growth of Giardia lamblia. The most active compound, disulfiram (Antabuse), was cidal at 1.23 ± 0.32 μM. In the adult mouse model, significant in vivo activity was demonstrated by increased cure rates and decreased parasite burdens. 相似文献
998.
The effects of nicotine and caffeine at dose levels approximating human consumption on skeletal development was investigated in Sprague-Dawley rats. One group of animals received nicotine administered subcutaneously by an Alzet miniosmotic pump from gestational d 6 through 12 (25 mg over 7 d; rate 149 micrograms/h). Control animals received physiological saline in a similar manner. A2. group received a single intravenous injection of caffeine (25 mg/kg) on gestational d 6. Control animals were treated with physiological saline. A further group received both nicotine and caffeine on gestational d 6 as described for the 2 previous groups. Evaluation of the fetal skeletal system on gestational d 20 revealed a significant decrease in the number of complete sternal ossification centers, as well as delays in the ossification of the skull and face, following combined nicotine and caffeine treatment. Other skeletal anomalies and variations were frequently observed, but their incidences were not significantly increased in any of the treatment groups, compared to the corresponding controls. A coteratogenic effect is evident between nicotine and caffeine on account of the increased incidence of delayed and abnormal skeletal development. 相似文献
999.
Impaired cardiac excitation-contraction coupling in ventricular myocytes from Ames dwarf mice with IGF-I deficiency. 总被引:1,自引:0,他引:1
Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are involved in the regulation of cardiovascular function. GH/IGF-I deficiency is associated with impaired cardiac performance manifested as reduced left ventricular ejection fraction and diastolic filling. This study was to determine the impact of IGF-I deficiency on single cardiac myocyte excitation-contraction (E-C) coupling. Ventricular myocytes were isolated from adult Ames dwarf mice and age-matched wild-type siblings. Dwarf mice are characterized by severe IGF-I deficiency. Mechanical properties were evaluated using a video edge detection system. Myocytes were electrically stimulated at 0.5 Hz. The contractile properties analysed included peak shortening (PS), time to peak shortening (TPS) and time to 90% relengthening (TR(90)), and maximal velocities of shortening/relengthening (+/-d L/d t). Intracellular Ca(2+) transients were evaluated by fura-2 fluorescence microscopy. Dwarf mice exhibited significantly reduced body and heart weights and severely deficient plasma IGF-I. Myocytes from dwarf mice displayed significantly smaller cell lengths (CLs), prolonged TPS/TR(90) and reduced +/-d L/d t compared with the wild-type littermates. The absolute PS was similar although PS/CL was enhanced in the dwarf group. Myocytes from dwarf animals displayed reduced peak intracellular Ca(2+) levels and slowed intracellular Ca(2+) clearing associated with a comparable resting intracellular Ca(2+). Furthermore, myocytes from the dwarf hearts were equally responsive to an elevation in extracellular Ca(2+) and exhibited an augmented stepwise decrease in response to minimal increase in stimulating frequencies compared with those from the wild-type group. These results suggest that deficiency in IGF-I may be directly associated with cardiac E-C coupling dysfunction at the ventricular myocyte level. 相似文献
1000.
Control of isometric muscle activity in cerebral palsy 总被引:6,自引:6,他引:0
Voluntary control of muscle contraction was examined in five adults with cerebral palsy, who were required to track a moving target by continuously varying the level of isometric contraction of elbow flexor muscles (measured by EMG). First performance varied from minimal control to almost normal control, depending on the severity of disability. Practice over 12 weeks reduced inappropriate muscle activity in the most disabled patients, but there was no increase in appropriate muscle activity for any patient beyond that observed after the first few minutes of tracking. Thus their ability to translate a visual response into the appropriate motor activity was impaired, and there was no evidence of potential to overcome this. This supports the authors' earlier proposal that impairment of sensory-motor learning is the primary cause of functional disability in cerebral palsy. The EMG tracking task may provide a technique for assessing the ability of individuals with cerebral palsy to control muscle contraction. 相似文献