Ki- 1-positive anaplastic large cell lymphoma of the masticator space with intracranial extension.

Ki- 1-positive anaplastic large cell lymphoma (ki- 1 ALCL) is a recently recognized form of non-Hodgkin lymphoma occurring predominantly in the skin and/or peripheral lymph nodes. We report the clinical, pathologic, and radiographic findings of a case of Ki- 1 ALCL arising within the masticator space and extending intracranially into the epidural space.     

AMBER: a scanning multiple-beam equalization system for chest radiography

Conventional chest radiography is limited by the small useful exposure range of radiographic film. The wide variation in absorption thickness of different parts of the chest results in areas of under- and over-exposure. An advanced multiple-beam equalization system, AMBER, controls local exposure delivered to the film. The system has a row of 20 modulators in front of the x-ray tube, each able to change the height of the local slit beam during scanning. Changes are made in response to measurements from a linear detector array in front of the film cassette. This array consists of 20 individually functioning detectors coupled through electronic feedback loops to the 20 modulators. A scan is obtained in 0.8 second with a local exposure time of approximately 50 msec. AMBER results in radiographs with significantly improved exposure of the mediastinum without overexposure of the lungs.     

Sound localization and use of binaural cues by the gerbil (Meriones unguiculatus)

Noise-localization thresholds and the ability to localize pure tones at 60 degrees separation were determined for gerbils. The gerbils were trained using a two-choice procedure with observing response in which the gerbils made a left or right response to sounds emanating from their left or right side in order to obtain food. The average 75% correct localization threshold of 7 gerbils for a 100-ms noise burst was 27 degrees with chance performance (p greater than .01) reached at 12 degrees. The ability of 4 gerbils to localize both low- and high-frequency pure tones indicates that gerbils are able to use both phase- and intensity-difference locus cues. The frequency at which tone localization was poorest was 2.8 kHz, well below the theoretical frequency of ambiguity of the phase cue but within the frequency range at which phase locking declines in the mammalian auditory system. The sound localization ability of gerbils is typical of small rodents, and there is no obvious sign that it is affected by the degenerative disorder of the central auditory system which has been recently discovered in gerbils.     

Involvement of CD31 in lymphocyte-mediated immune responses: importance of the membrane-proximal immunoglobulin domain and identification of an inhibiting CD31 peptide

CD31 (PECAM-1) is an immunoglobulin gene superfamily cell adhesion molecule found on vascular endothelium, platelets, and leukocytes. Lymphocyte expression of CD31 is most closely associated with the CD45RA+CD8+ naive T phenotype. CD31 has recently been shown to play a role in leukocyte egress to inflammatory sites. The mechanism of CD31 adhesion remains under investigation. Several investigators have reported evidence for a heterotypic ligand. We have previously shown that CD31 is phosphorylated with cell activation, which suggests a possible role for CD31 in cell activation events. We therefore studied the effects of CD31 antibodies on in vitro assays of lymphocyte activation. One CD31 antibody, LYP21, inhibited the mixed lymphocyte reaction (MLR) in a specific and dose-dependent fashion. An LYP21 epitope was localized to the sixth Ig domain of CD31. This peptide and a scrambled control peptide were synthesized and used to study effects of this epitope on lymphocyte activation. The CD31 peptide strongly inhibited the MLR. Because CD31 is expressed on both stimulator and responder populations, stimulator peripheral blood leukocytes and responder lymphocyte populations were separately incubated with CD31 peptide or control peptide and then washed before mixing. The CD31 peptide inhibited the MLR equally when either stimulator or responder cells were preincubated with the CD31 peptide. We further sorted responder cells into CD31-high and CD31-low populations and separately incubated these subsets with peptides. The CD31 peptide strongly inhibited MLRs, regardless of level of responder-cell CD31 expression. Examination of MLR reactions involving the CD31 peptide showed dispersed small aggregates of cells, rather than the single large aggregate observed in control MLRs. The CD31 peptide did not affect activation of lymphocytes by phorbol myristate acetate (PMA) and ionomycin. These results suggest that a surface CD31-ligand interaction may have a functional role in alloimmune lymphocyte activation and identify a functionally important domain of CD31.