血清碱性成纤维细胞生长因子检测在急性淋巴细胞白血病中的临床意义

目的　探讨急性淋巴细胞白血病 (ALL)患者血清碱性成纤维细胞生长因子 (bFGF)水平及其临床意义。方法　应用酶联免疫法(ELISA)检测 26例ALL初发未治患者及 5例复发患者化疗前后血清bFGF的水平,并与正常对照组比较。结果　ALL初治患者血清bF GF水平为 (64. 71±27. 45 )pg/L,复发患者血清bFGF水平为 ( 76. 02±27 07 )pg/L,均明显高于正常人对照组水平 ( 24 06±5. 97 )pg/L,差异有非常显著性意义 (P<0. 001, 0. 05 );化疗后完全缓解 (CR)患者血清bFGF水平 [ (29. 64 ±7. 02 )pg/L]明显下降,与对照组相比无显著性差异 (P>0 05 ),经化疗无效 (NR)患者血清bFGF水平 [ 76. 16±29. 83 )pg/L]与化疗前水平 [ ( 82. 11±32. 64 )pg/L]相比无显著性差异 (P>0. 05 );NR组化疗前血清bFGF水平明显高于CR组化疗前的水平 [ 50. 36±12. 52 )pg/L],P<0. 01。结论　ALL患者存在bFGF的异常高表达,检测血清bFGF对ALL的疗效观察和预后判断有一定临床价值。     

陕西地区汉族人慢性乙型肝炎与HLA-DRB1基因的相关性研究

目的研究HLA-DRB1等位基冈与HBV感染慢性化的相关性。方法用PCR-SSP方法对陕西地区汉族人群中的慢性乙型肝炎（CHB）患者54例、乙型肝炎表面抗原携带者（ASC）32例及正常健康人108人进行HLA-DRB1等位基因分型比较,并对HLA-DRB1等位基因分型与HBV不同复制状态进行相关性分析。结果陕西地区正常汉族人HLA-DRB1等位基因以DRB1＊04（16．2％）,DRB1＊09（12.5％）,DRB1＊12（11．6％）,DRB1＊15（13．4％）最为常见;CHB组HLA-DRB1＊03的等位基因频率（11．1％）明显高于健康对照组（3．7％）,两组相比差异显著（Pc=0．014;OR=3．57;P〈0．05）;DRB1＊07基因位点在HBV高复制状态较低复制状态多见（Pc=0．034,OR=3．88,P〈0．05）;HLA-DRB1＊15等位基因频率对照组为13．4％,明显高于CHB组7．4％（Pc=0．039;OR=0．48;P〈0．05）。结论HLA-DRB1＊03、HLA-DRB1＊07与陕西地区汉族人HBV感染后的慢性化相关联,HLA-DRB1＊15在陕西地区汉族人与HBV感染的保护相关;HLA-Ⅱ类基因是决定HBV感染后临床转归的重要因素之一。     

阴性或阳性症状为主的精神分裂症患者的P300亚成份分析

目的探讨P300亚成份在以阴性或阳性症状为主的精神分裂症患者认知障碍评定中的价值。方法对56例以阴性症状为主的患者（阴性组）和49例以阳性症状为主的患者（阳性组）分别进行P3a及P3b检测,比较其与正常对照组的差异。结果阴性组在FZ点的平均P3a潜伏期较阳性组显著延长（ P＜0.01）、FZ和CZ点的平均P3a波幅及CZ和PZ点的平均P3b波幅显著低于阳性组与正常对照组（分别P＜0.01和P＜0.05）。结论以阴性症状为主的精神分裂症患者的认知功能损伤可能比以阳性症状为主者严重,新奇刺激P3a对于以阴性症状为主的患者的认知功能评估更有意义。     

硫酸镁治疗婴幼儿肺炎并心力衰竭及中毒性脑病的疗效

目的探讨硫酸镁对婴幼儿肺炎合并心力衰竭及中毒性脑病的临床疗效。方法 74例肺炎合并心力衰竭及中毒性脑病患儿随机分为治疗组44例,对照组30例,观察2组患儿治疗后控制惊厥、呼吸转规则、神志恢复所需时间以及2组患儿痊愈率及后遗症发生率等。结果治疗组患儿心衰纠正、惊厥控制、呼吸转规则、神志恢复时间均较对照组缩短,2组相比差异有统计学意义,P<0.05。结论硫酸镁对小儿肺炎合并心力衰竭及中毒性脑病疗效可靠,且安全简便。     

Reverse Engineering of Biochemical Reaction Networks Using Co-evolution with Eng-Genes

A major challenge when attempting to model biochemical reaction networks within the cell is that the dimensionality can become huge, where a large number of molecular species can be involved even in relatively small networks. This investigation attempts to infer models of these networks using a co-evolutionary algorithm that reverse engineers differential equation models of the target system from time-series data. The algorithm not only estimates the system parameters, but also the symbolic structure of the network. To reduce the problem of dimensionality, the algorithm uses a partitioning method while integrating candidate models in order to decouple system equations. In addition, the conventional evolutionary algorithm has been modified and extended to include a technique called ‘eng-genes’, where candidate models are built up from fundamental mathematical terms derived from knowledge about the target system a priori. This technique essentially focuses the search on more biologically plausible models. The approach is demonstrated on several example reaction networks. The results show that the eng-genes method of limiting the term pool using a priori knowledge improves the convergence of the reverse engineering process compared with the conventional method, resulting in more accurate and transparent models.     

Isolation and characterization of 23 microsatellite loci in the Chinese sucker (Myxocyprinus asiaticus)

Chinese sucker (Myxocyprinus asiaticus) is a second class state-protected animal in China. In this study, we developed twenty-three polymorphic microsatellite loci in Chinese sucker. The observed and expected heterozygosity ranged from 0.292–0.958 to 0.423–0.900, respectively. The polymorphic information content ranged from 0.356 to 0.869, with a mean of 0.710. These microsatellite loci are expected to be useful for further studies of genetic diversity, population genetic structure, and assessments of the artificial propagation release effect of Chinese sucker.     

Isolation and characterization of twenty-six microsatellite loci for the tetraploid fish Dabry’s sturgeon (Acipenser dabryanus)

To evaluate the population genetic diversity as a means of devising conservation strategies, we constructed (CA)n and (CT)n enriched genomic libraries for Acipenser dabryanus, a narrowly endemic and endangered species in China. Twenty-six polymorphic microsatellite markers were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Between 3 and 13 alleles per locus were observed. Mean expected heterozygosities (H _E) and Shannon-Wiener Diversity Indices (H′) per locus ranged from 0.303 to 0.806, and from 0.480 to 1.893, respectively. The microsatellite markers described here are valuable tools for the population genetics research of A. dabryanus.     

Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs

The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100-300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.     

Early biliary complications of laparoscopic cholecystectomy: evaluation on T2-weighted MR cholangiography in conjunction with mangafodipir trisodium-enhanced 3D T1-weighted MR cholangiography

OBJECTIVE: Our aim was to assess preliminary experience with combined conventional T2-weighted and mangafodipir trisodium (MnDPDP)-enhanced T1-weighted MR cholangiography in evaluating early biliary complications of laparoscopic cholecystectomy. SUBJECTS AND METHODS: Conventional heavily T2-weighted MR cholangiography with MnDPDP-enhanced T1-weighted MR cholangiography and ERCP were performed in seven patients with high clinical suspicion of biliary complications after laparoscopic cholecystectomy. The final diagnoses of complications were classified according to the presence and degree of bile duct injury, bile leakage, and retained stones. RESULTS: The diagnoses on MR cholangiography were as follows: complete transection and occlusion of the common bile duct with bile leakage (n = 3), partial strictures of the common bile duct with bile leakage (n = 1), cystic duct leakage (n = 1), partial ligation of an aberrant right hepatic duct (n = 1), and hemorrhage without biliary complication (n = 1). The final diagnoses at surgery (n = 2) and ERCP (n = 5) were as follows: complete transection and occlusion of the common bile duct with bile leakage (n = 2), partial strictures of the common bile duct with bile leakage (n = 2), cystic duct leakage (n = 1), partial ligation of an aberrant right hepatic duct (n = 1), and hemorrhage without biliary complication (n = 1). MR cholangiography accurately yielded the same findings as the final diagnoses, except in one case with partial stricture of the bile duct with bile leakage (overdiagnosed as complete occlusion on MR cholangiography). CONCLUSION: Combined conventional T2-weighted and MnDPDP-enhanced T1-weighted MR cholangiography may eliminate the use of other studies for the imaging of biliary complications after cholecystectomy if this preliminary data can be verified in a larger study.     

基于Bevilacqua模型的乳腺癌保乳术患者术后淋巴水肿风险预测的临床研究

目的验证Bevilacqua乳腺癌术后淋巴水肿风险预测模型的临床适用性及可行性。方法回顾性分析2010年1月至2015年12月203例乳腺癌患者临床资料,临床数据分析使用统计学软件SPSS 24.0。Cox回归模型分析乳腺癌患者术后发生上肢淋巴水肿的危险因素,以P<0.05为有统计学意义;绘制ROC曲线,以曲线下面积检验模型预测效果;应用Hosmere-Lemeshow检验评估预测值与实际值的校准程度,以P>0.05为预测模型校准能力较好,预测与实际没有区别。结果所有患者随访共计62~86个月,中位随访时间70个月。术后5年内共发生上肢淋巴水肿患者45例(22.2%)。Cox回归模型分析结果显示,高身体质量指数(BMI)、接受过新辅助化疗、全腋窝淋巴结清扫、接受过放疗是上肢淋巴水肿的独立危险因素。Becilacqua上肢淋巴水肿风险预测模型ROC曲线分析结果显示,模型AUC值为0.711,95%CI(0.651~0.760),有较好的的预测效果。Hosmer-Lemeshow检验结果显示,风险预测模型预测风险与实际无明显差异(P=0.262),校准能力较好,与实际差别不大。结论Bevilacqua术后6个月淋巴水肿风险预测模型的准确性及适用性较高,可用于临床对乳腺癌保乳术后淋巴水肿的预测,可为预防淋巴水肿的发生制定干预决策提供参考。