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目的:研究胎儿对口腔病原体的炎性和免疫反应及其与早产的关系。研究设计:前瞻性收集640例脐血样本。采用ELISA测定脐血中C-反应蛋白、IL-1β、IL-6、肿瘤坏死因子(TNF)-α、前列腺素E2(PGE2)及8-异前列腺素的水平,并将测定结果按中位数分为高水平组(〉中位数)和低水平组(≤中位数)。应用免疫印迹检测板检测胎儿体内口腔病原体的IgM抗体。发现≥1个口腔病原体的特异抗体定为阳性。早产定义为孕〈35周的自然分娩。应用X^2检验计算脐血血清介质或IgM水平与早产的关系。按不同介质和IgM水平分层计算早产时的OR值。结果:在640例分娩孕妇中,48例(7.5%)早产。按8-异前列腺素和TNF-α【的水平分别分为高、低水平两组,其早产率分别为23% vs5%(p〈0.001),10%vs4%(P〈0.01)。IgM阳性组的早产率较阴性组高(10.6%vs5.8%,P=0.04)。 相似文献
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Wang JF; Bashir M; Engelsberg BN; Witmer C; Rozmiarek H; Billings PC 《Carcinogenesis》1997,18(2):371-375
Chromium (Cr) is a human carcinogen and a potent DNA damaging agent.
Incubation of DNA with CrCl3 resulted in dose-dependent binding of Cr to
DNA and, at concentrations >20 microM, altered the electrophoretic
mobility of a 100 bp oligonucleotide. We also demonstrate that high
mobility group (HMG) proteins 1 and 2 bind Cr-damaged DNA (Cr-DNA). Protein
binding was lesion density-dependent, with maximal binding to DNA treated
with 100 microM CrCl3. HMG2 binds to Cr-DNA with a calculated Kd of
approximately 10(-9) M. These proteins also bound DNA obtained from
chromate-treated cells. These results suggest that the covalent attachment
of Cr to DNA induces alterations in DNA structure which are recognized by
HMG1 and HMG2. Therefore, these proteins may function as Cr-damaged DNA
recognition proteins in vivo and as a consequence of binding, may play a
role in directing the cellular response to Cr-DNA adduct formation.
相似文献
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P Greally MJ Hussein AJ Cook AP Sampson PJ Piper JF Price 《Archives of disease in childhood》1993,68(3):389-392
It is postulated that a vigorous host inflammatory response in the cystic fibrosis lung contributes to lung injury. Tumour necrosis factor-alpha (TNF-alpha) may play a part in that process and in the generation of leukotrienes. Therefore, the relationships between sputum TNF-alpha, leukotriene concentration, and lung function abnormalities in 16 children with cystic fibrosis were investigated. Each subject provided sputum samples and performed spirometry. TNF-alpha was measured by enzyme linked immunosorbent assay; individual leukotrienes were separated using high performance liquid chromatography and quantified by radioimmunoassay. The geometric mean concentration of TNF-alpha was 129.7 pg/ml and 95% confidence interval 48.2 to 348.3. Mean (SEM) leukotriene B4 (LTB4) was 97.8 (22.9) pmol/g and total cysteinyl leukotrienes were 60.9 (14.8) pmol/g. Mean (SD) forced expiratory volume in one second (FEV1) of the group was 53 (15)% of predicted and forced vital capacity (FVC) was 65 (14)% of predicted. There was a significant positive correlation between TNF-alpha and both LTB4 and the total cysteinyl leukotriene sputum content. An inverse relationship existed between TNF-alpha and FEV1 and FVC. Moreover, a negative correlation was observed between sputum LTB4 and FEV1 and FVC. These results suggest that TNF-alpha and the leukotrienes may participate in the airways inflammation and airflow obstruction observed in cystic fibrosis subjects and support the hypothesis that TNF-alpha upregulates the 5-lipoxygenase pathway in vivo. 相似文献
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The cytokines interleukin-1 and interleukin-2 participate in the inflammatory response, and may contribute to hypergammaglobulinaemia G and the development of lung injury in cystic fibrosis. Anti-inflammatory treatment with corticosteroids may attenuate this response. The effect of a 12 week course of oral prednisolone on spirometry and serum concentrations of interleukin-1 alpha (IL-1 alpha), soluble interleukin-2 receptor (sIL-2R), and IgG was investigated in 24 children with cystic fibrosis. Prednisolone was administered, in a double blind and placebo controlled manner, at an initial dose of 2 mg/kg daily for 14 days and tapered to 1 mg/kg on alternate days for 10 weeks. The treated group (n = 12) experienced an increase in forced expiratory volume in one second and forced vital capacity at 14 days, however, these changes were smaller at 12 weeks. In the treated group, change in pulmonary function was associated with decreased serum IgG and cytokine concentrations. Prednisolone suppresses serum concentrations of these cytokines, which may participate in the inflammatory response, the excessive synthesis of IgG, and airflow obstruction observed in cystic fibrosis patients. 相似文献
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C. Richer J. Gobert M. Noyer E. Wülfert and JF Giudicelli 《Fundamental & clinical pharmacology》1996,10(6):529-537
Summary— Mivazerol is a new compound that could potentially reduce perioperative cardiovascular morbidity and mortality in patients with or at risk of coronary disease and submitted to surgery. This action of mivazerol depends on a well documented centrally mediated reduction in sympathetic nerve activity, but a direct peripheral decrease in sympathetic neurotransmitter release induced by activation of prejunctional α2-adrenoceptors located on sympathetic nerve endings could also contribute. To investigate this issue, the effects of mivazerol on the pressor, systemic and regional hemodynamic (pulsed Doppler technique) as well as on the cardiac responses to electrical stimulation of the spinal cord (SCS) were measured in pithed rats in the absence and in the presence of mivazerol. Mivazerol exerted strong sympathoinhibitory effects: SCS-induced increases in blood pressure, total peripheral resistance and heart rate were dose-dependently reduced by mivazerol, but among the regional vascular beds investigated, only the hindlimb vasoconstrictor responses were significantly drug-affected. All these sympathoinhibitory effects of mivazerol were abolished by prior yohimbine administration. Simultaneously, mivazerol did not induce any postjunctional adrenoceptor blockade as it did not affect noradrenaline cardiac and hemodynamic effects. On the contrary, through postjunctional α2-adrenoceptor stimulation, mivazerol, in this pithed preparation, dose-dependently increased blood pressure, total peripheral and hindlimb vascular resistances, but heart rate was not affected. We conclude that, in the pithed rat, mivazerol exerts strong peripheral sympathoinhibitory effects. The mechanism involved is prejunctional α2-adrenoceptor activation as i) mivazerol does not display any postsynaptic α-adrenoceptor blocking effect — it even behaves as a postsynaptic α2-adrenoceptor agonist — and ii) yohimbine abolishes mivazerol's sympathoinhibitory effects. Thus, direct peripheral together with central mechanisms contribute to mivazerol's sympathoinhibitory effects and ultimately to its cardioprotective action. 相似文献
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