全文获取类型
收费全文 | 429篇 |
免费 | 47篇 |
国内免费 | 132篇 |
专业分类
儿科学 | 8篇 |
妇产科学 | 3篇 |
基础医学 | 86篇 |
口腔科学 | 4篇 |
临床医学 | 94篇 |
内科学 | 94篇 |
皮肤病学 | 13篇 |
神经病学 | 3篇 |
特种医学 | 2篇 |
外科学 | 41篇 |
综合类 | 40篇 |
预防医学 | 41篇 |
眼科学 | 4篇 |
药学 | 79篇 |
中国医学 | 1篇 |
肿瘤学 | 95篇 |
出版年
2022年 | 2篇 |
2021年 | 4篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 5篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 3篇 |
2013年 | 8篇 |
2012年 | 17篇 |
2011年 | 15篇 |
2010年 | 5篇 |
2009年 | 12篇 |
2008年 | 9篇 |
2007年 | 86篇 |
2006年 | 23篇 |
2005年 | 36篇 |
2004年 | 8篇 |
2003年 | 11篇 |
2002年 | 14篇 |
2001年 | 20篇 |
2000年 | 22篇 |
1999年 | 30篇 |
1998年 | 18篇 |
1997年 | 15篇 |
1996年 | 15篇 |
1995年 | 11篇 |
1994年 | 8篇 |
1993年 | 14篇 |
1992年 | 29篇 |
1991年 | 15篇 |
1990年 | 18篇 |
1989年 | 12篇 |
1988年 | 18篇 |
1987年 | 8篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1984年 | 12篇 |
1983年 | 7篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1970年 | 2篇 |
排序方式: 共有608条查询结果,搜索用时 205 毫秒
1.
2.
赛赓啶对 KBV200细胞多药抗性的逆转作用 总被引:3,自引:0,他引:3
研究赛赓啶对KBV200细胞多药抗性的逆转作用及逆转机制。在KBV200细胞,采用MTT法,测出赛赓啶对长春新碱、阿霉素和鬼臼乙叉甙耐药的逆转系数分别为5.5,2.0和1.9,而对5-氟尿嘧啶、美法仑的细胞毒性作用无明显影响,表明赛赓啶为多药抗性逆转剂。荧光分光光度法测定表明,赛赓啶可使KBV200细胞内阿霉素蓄积量增加。流式细胞荧光测定显示赛赓啶可增加罗丹明123的蓄积并减慢其外排。免疫细胞化学及狭缝杂交表明赛赓啶不影响KBV200细胞的P-糖蛋白染色深度和 mdr1 RNA 表达水平。以上结果提示赛赓啶的多药抗性逆转机制是抑制P-糖蛋白泵的功能。 相似文献
3.
4.
G Blancho R Josien D Douillard J D Bignon A Cesbron J P Soulillou 《Transplantation》1992,54(5):871-874
We examined the prevalence of cytomegalovirus infectious episodes, as defined by clinical, virological, and serological criteria (i.e., CMV disease), in 660 kidney graft recipients; 109 patients (16.5%) developed the disease, and 551 did not. No significant statistical link between CMV disease prevalence and a given HLA-A, -B, or -DR allele was observed. However, patients with HLA-DR7 matched grafts were statistically more frequently found (P < 0.01) in the group of recipients who developed CMV disease as compared with the group who did not develop CMV disease. Furthermore, among patients who developed CMV disease, a significant increase of HLA-DR7 matched over DR7 mismatched patients was noted, whereas no difference between matched and mismatched recipients for the other HLA-DR alleles was found. No difference in the severity of graft failure, often observed during, or immediately after, the CMV episode, was noted between patients matched or mismatched for HLA-DR7. Our data suggest that donor/recipient matching for HLA-DR7 is associated with increased CMV disease. 相似文献
5.
Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization. 相似文献
6.
7.
8.
9.
P Chariot B Couste F Guillon A Gaudichet J Bignon P Brochard 《The American review of respiratory disease》1992,146(1):61-65
It is recognized that bronchoalveolar lavage (BAL) gives access to particulate matter present at the surface of the peripheral airspace. The objective of the present study was to evaluate the ability of BAL fluid analysis to predict the lung parenchymal particulate content. A BAL fluid sample, the parenchyma sample having undergone BAL, and an adjacent parenchyma sample that had not undergone BAL were obtained at autopsy on 10 individuals without any known recent occupational exposure to mineral particles. The particles (larger than 0.1 micron) were analyzed using a transmission electron microscope equipped with a microanalysis system. Nineteen types of particles were distinguished. The distribution of particle types in the three samples was compared. No significant difference between the relative concentrations was found, except for two particle types: fly ash (excess in BAL fluid compared with lavaged lung) and kaolinite (excess in lavaged lung compared with adjacent area). Such differences may be due to limitations in methodology. Although no correlation could be found between the absolute concentrations of particles in BAL fluid and in lung tissue, analysis of particles in BAL fluid may provide information on the types of particles present in the lung parenchyma. 相似文献
10.
The applications of molecular biology diagnosis in oncology are presented and discussed. These are: improved nosologic definition; diagnosis of clonality; definition of new prognosis subgroups in some cancers; as an aid in bone marrow transplantation follow-up; diagnosis of the residual disease; early diagnosis of cancer; evaluation of individual cancer risk. 相似文献