全文获取类型
收费全文 | 58228篇 |
免费 | 4623篇 |
国内免费 | 207篇 |
专业分类
耳鼻咽喉 | 741篇 |
儿科学 | 1350篇 |
妇产科学 | 1372篇 |
基础医学 | 7968篇 |
口腔科学 | 1181篇 |
临床医学 | 5831篇 |
内科学 | 11957篇 |
皮肤病学 | 1224篇 |
神经病学 | 5854篇 |
特种医学 | 2248篇 |
外国民族医学 | 2篇 |
外科学 | 9823篇 |
综合类 | 810篇 |
一般理论 | 82篇 |
预防医学 | 3824篇 |
眼科学 | 1078篇 |
药学 | 3747篇 |
中国医学 | 86篇 |
肿瘤学 | 3880篇 |
出版年
2023年 | 510篇 |
2022年 | 994篇 |
2021年 | 1875篇 |
2020年 | 1135篇 |
2019年 | 1506篇 |
2018年 | 1700篇 |
2017年 | 1191篇 |
2016年 | 1382篇 |
2015年 | 1562篇 |
2014年 | 2161篇 |
2013年 | 2553篇 |
2012年 | 4088篇 |
2011年 | 4062篇 |
2010年 | 2240篇 |
2009年 | 1960篇 |
2008年 | 3209篇 |
2007年 | 3254篇 |
2006年 | 3036篇 |
2005年 | 2854篇 |
2004年 | 2646篇 |
2003年 | 2436篇 |
2002年 | 2263篇 |
2001年 | 888篇 |
2000年 | 899篇 |
1999年 | 858篇 |
1998年 | 548篇 |
1997年 | 460篇 |
1996年 | 362篇 |
1995年 | 346篇 |
1994年 | 299篇 |
1993年 | 296篇 |
1992年 | 576篇 |
1991年 | 565篇 |
1990年 | 569篇 |
1989年 | 478篇 |
1988年 | 445篇 |
1987年 | 444篇 |
1986年 | 413篇 |
1985年 | 471篇 |
1984年 | 370篇 |
1983年 | 312篇 |
1982年 | 241篇 |
1981年 | 227篇 |
1980年 | 237篇 |
1979年 | 291篇 |
1978年 | 250篇 |
1976年 | 204篇 |
1974年 | 245篇 |
1973年 | 210篇 |
1972年 | 206篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
The development of the eye was investigated in the mouse embryo following a single administration of ethanol plus [3H]thymidine to the dam on day 13 of gestation. After 1 hr there was no difference in the number of labelled cells/100 micron 2 in the neural layer of the retina compared to controls, but there was an alcohol-related reduction in labelling density. After 24 hr there was an increase in the numbers of both pyknotic cells and mitotic figures, breaks occurred in the inner surface of the retina and cell debris was being extruded into the posterior chamber. At 48 hr the increase in pyknotic cells persisted, but there was less evidence of cell debris and the borders had been repaired. The estimated cell cycle time in the neural progenitor cells following maternal alcohol administration was increased 7-fold compared to controls. Morphometric analysis revealed that after 48 hr there were significant alcohol-related reductions in the width and depth of the eye, in the thickness of the neural layer and in the interocular distance. It appears that many of the ophthalmic abnormalities reported in human fetal alcohol syndrome can be produced in the mouse embryo following a single episode of acute maternal intoxication during a critical period of ocular ontogeny, and that they evolve primarily from disturbances in the normal patterns of recruitment and loss of neural progenitor cells in the developing retina. 相似文献
52.
Pharmacological properties of fluphenazine-mustard, an irreversible calmodulin antagonist 总被引:1,自引:0,他引:1
We describe an improved synthesis and properties of fluphenazine-mustard, a potent phenothiazine having an alkylating chlorethylamine chain in its structure. The drug possesses anticalmodulin activity equivalent to the parent compound, but unlike fluphenazine dihydrochloride, the mustard derivative irreversibly antagonizes the ability of calmodulin to activate cyclic nucleotide phosphodiesterase. This property is partially calcium-dependent and can be overcome by coincubation with excess fluphenazine dihydrochloride. The compound irreversibly inactivated calmodulin when incubated with intact cells and caused single-stranded breakage of DNA. Fluphenazine-mustard possesses potent antiproliferative and cytotoxic properties against malignant cell lines that are likely to be mediated through both of these actions. 相似文献
53.
54.
Georgios K Glantzounis Henryk J Salacinski Wenxuan Yang Brian R Davidson Alexander M Seifalian 《Liver transplantation》2005,11(9):1031-1047
Oxidative stress is an important factor in many pathological conditions such as inflammation, cancer, ageing and organ response to ischemia-reperfusion. Humans have developed a complex antioxidant system to eliminate or attenuate oxidative stress. Liver ischemia-reperfusion injury occurs in a number of clinical settings, including liver surgery, transplantation, and hemorrhagic shock with subsequent fluid resuscitation, leading to significant morbidity and mortality. It is characterized by significant oxidative stress but accompanied with depletion of endogenous antioxidants. This review has 2 aims: firstly, to highlight the clinical significance of liver ischemia-reperfusion injury, the underlying mechanisms and the main pathways by which the antioxidants function, and secondly, to describe the new developments that are ongoing in antioxidant therapy and to present the experimental and clinical evidence about the role of antioxidants in modulating hepatic ischemia-reperfusion injury. 相似文献
55.
56.
57.
Andreas G Schatzlein Bernd H Zinselmeyer Adurrahim Elouzi Christine Dufes Ya Tsz A Chim Clive J Roberts Martyn C Davies Avril Munro Alexander I Gray Ijeoma F Uchegbu 《Journal of controlled release》2005,101(1-3):247-258
Previously, the lower generation (DAB 8-generation 2 and DAB 16-generation 3) polypropylenimine dendrimers have been shown to be effective gene delivery systems in vitro. In the current work, we sought to: (a) test the effect of the strength of the carrier, DNA electrostatic interaction on gene transfer and (b) to study the in vivo gene transfer activity of these low molecular weight (<1687 Da) non-amphiphilic plain and quaternary ammonium gene carriers. Towards this aim, methyl quaternary ammonium derivatives of DAB 4 (generation 1), DAB 8, DAB 16 and DAB 32 (generation 4) were synthesised to give Q4, Q8, Q16 and Q32, respectively. Quaternisation of DAB 8 proved to be critical in improving DNA binding, as evidenced by data from the ethidium bromide exclusion assay and dendrimer-DNA colloidal stability data. This improved colloidal stability had a major effect on vector tolerability, as Q8-DNA formulations were well tolerated on intravenous injection while a similar DAB 8-DNA dose was lethally toxic by the same route. Quaternisation also improved the in vitro cell biocompatibility of DAB 16-DNA and DAB 32-DNA dendrimer complexes by about 4-fold but not that of the lower generation DAB 4-DNA and DAB 8-DNA formulations. In contrast to previous reports with non-viral gene delivery systems, the intravenous administration of DAB 16-DNA and Q8-DNA formulations resulted in liver targeted gene expression as opposed to the lung targeted gene expression obtained with the control polymer-Exgen 500 [linear poly(ethylenimine)] and a lung avoidance hypothesis is postulated. We conclude that the polypropylenimine dendrimers are promising gene delivery systems which may be used to target the liver and avoid the lung and also that molecular modifications conferring colloidal stability on gene delivery formulations have a profound effect on their tolerability on intravenous administration. 相似文献
58.
Simultaneous angiographic late stent thrombosis in two different coronary vessels after withdrawal of the combined anti-platelet therapy 总被引:2,自引:2,他引:0
59.
60.
Age-related bone loss has been associated with high levels of marrow adipogenesis. Estrogens (E2) are known to regulate the differentiation of marrow precursors into osteoblasts, however, their role in bone marrow adipogenesis
remain unknown. E2 regulate adipocyte differentiation in subcutaneous and visceral fat through interaction with other nuclear receptors. This
interaction has not been assessed in bone marrow adipocytes in vivo. In this study, we compared two groups of animals, young
and old, after either oophorectomy (OVX) or oophorectomy plus E2 (OVX + E2) replacement. We found that absence of E2 was associated with higher levels of PPARγ and lower levels of Sirt1 most significantly in the old group. In addition, old
mice responded better to E2 replacement in terms of reducing adipogenesis and PPARγ expression as well as increasing levels of Sirt1 expression. Our
findings represent a new understanding of the role of E2 in age-related bone loss, which could be mediated through the regulation of Sirt1 expression within the bone marrow. In addition,
this evidence suggests that old individuals may show a better response to E2 administration in terms of reverting the high levels of marrow fat seen in age-related bone loss. 相似文献