儿童扁桃体切除前后血清中免疫球蛋白和循环免疫复合物的变化

扁桃体炎是儿童多发病,扁桃体切除是治疗扁桃体炎的主要手段。一般认为扁桃体具有一定的免疫功能,为探讨扁桃体切除后对儿童体液免疫有无     

胎儿的免疫抑制机理

哺乳动物为了保存自身,有高度完善的免疫功能,每一个体都会将非自身的成分作为异物排斥。这种免疫功能从种系生存的角度来看,可能具有排斥受精卵和附属物的作     

孔雀绿比色法测定巨噬细胞吞噬功能的研究

测定巨噬细胞(Mφ)吞噬功能是了解机体免疫功能的试验。本文利用无色孔雀绿测定血浆中微量的血红蛋白含量,对用孔雀绿比色法测定小鼠Mφ的吞噬功能进行了研究。实验结果表明小鼠腹腔细胞贴壁2小时,效靶细胞比例1∶100、吞噬时间1小时为该方法的最佳条件。同时,还用本法进行了胸腺素和氢化可的松对体外Mφ吞噬功能影响的测定,结果胸腺素组OD值明显高于对照组,而氢化可的松组OD值明显低于对照组,该方法操作简单,孔雀绿呈色稳定,是定量测定Mφ吞噬功能较满意的试验方法。     

抗甲状腺刺激性免疫球蛋白单链抗体在大肠杆菌中的表达

 目的 制备可溶性抗甲状腺刺激性免疫球蛋白(TSI)单链抗体。方法 构建的抗TSI单链抗体可溶性表达载体,转化E.coli XL 1-blue进行表达,表达产物经SDS-PAGE、Western blotting和ELISA检测,同时对表达条件作了初步探索。结果 抗TSI单链抗体可溶性表达于细菌培养上清和周质间隙,大小约30 KDa,具有抗TSI活性。温度、诱导物浓度及蔗糖对抗TSI单链抗体的表达产量有一定影响。结论 抗TSI单链抗体可溶性表达成功,为制备基因工程抗体奠定了基础。     

雷帕霉素纳米粒滴眼液联合环孢素A缓释膜治疗兔高危角膜移植术后免疫排斥反应的研究

Objective To evaluate the combined effect of topical rapamycin (RAPA) eye drop in nanometer vector and poly (lactic acid) (PLA) wafers of cyclospoirne A(CsA) in the prevention of acute allograft rejection after rabbit corneal transplantation. Methods It was an experimental study. RAPA was incorporated into the nanometer particles and CsA was incorporated into PLA wafers. A was syngeneic control whose both donor and recipient are New Zealand rabbit. Gray donor corneas were implanted into the 102 recipients of New Zealand albino rabbits with corneal neovascularization who were randomly divided into B, C, D, E, F, G 6 groups to receive the different types of therapy: B was no therapy control; C was eye drop of nanometer vector but no RAPA twice a day,28 days; D was PLA wafers in the anterior chamber of rabbit eyes but no drugs; E was 0.5% RAPA eye drop of nanometer vector twice a day,28 days; F was PLA wafers of CsA in the anterior chamber of rabbit eyes; G was PLA wafers of CsA in the anterior chamber of rabbit eyes and 0.5% RAPA eye drop of nanometer vector eye drop twice a day for 28 days together. Postoperative evaluation included slit-lamp biomicroscopy, histopathology and immunohistoiogy, Cytokines related with neovascularization and immunosuppression in the corneal tissue by RT-PCR. The graft survival was assessed by One-Way ANOVA and q test. Results Corneal allograft survival time: A (100.00±0.00), B ( 8.44±1.24), C (8.89±2.57), D (8.56±2.30), E (43.11±5.58), F (43.67±9.54), G (72.00±15.34)d. Group G led to a statistically significant prolongation of transplant survival and was superior than group E and F which was a statistical prolongation compared with group B, C and D (qGE=11.42, qGF=11.24,qEB=13.64, qEC=13.38, q<=13.46, qFB=13. 82, qFC=13.56, qFD=13.64; P<0.01). Immunohistopathologically, the grafts were subjected to an immune response contained a dense infiltrate of neutorphils,CD4+ and CD8+ T lymphocytes in the group B ,C and D. This cellular infiltrate was a significant reduction in group E,F,G. RT-PCR showed that the gene expression of IL-2 was inhibited earlier (3 days) in group F, G and VEGF gene expression being suppressed later (14 days) in group E, G. Conclusions Combined therapy with topical application of RAPA eye drop of nanometer vector and CsA PLA wafers can significantly prolong the survival of allograft at high-risk. Moreover, topical combined treatment of them is more effective, lower dosage, less side-effects and cheaper than the treatment with topical individual immunosuppressive drug.     

雷帕霉素纳米粒滴眼液联合环孢素A缓释膜治疗兔高危角膜移植术后免疫排斥反应的研究

Objective To evaluate the combined effect of topical rapamycin (RAPA) eye drop in nanometer vector and poly (lactic acid) (PLA) wafers of cyclospoirne A(CsA) in the prevention of acute allograft rejection after rabbit corneal transplantation. Methods It was an experimental study. RAPA was incorporated into the nanometer particles and CsA was incorporated into PLA wafers. A was syngeneic control whose both donor and recipient are New Zealand rabbit. Gray donor corneas were implanted into the 102 recipients of New Zealand albino rabbits with corneal neovascularization who were randomly divided into B, C, D, E, F, G 6 groups to receive the different types of therapy: B was no therapy control; C was eye drop of nanometer vector but no RAPA twice a day,28 days; D was PLA wafers in the anterior chamber of rabbit eyes but no drugs; E was 0.5% RAPA eye drop of nanometer vector twice a day,28 days; F was PLA wafers of CsA in the anterior chamber of rabbit eyes; G was PLA wafers of CsA in the anterior chamber of rabbit eyes and 0.5% RAPA eye drop of nanometer vector eye drop twice a day for 28 days together. Postoperative evaluation included slit-lamp biomicroscopy, histopathology and immunohistoiogy, Cytokines related with neovascularization and immunosuppression in the corneal tissue by RT-PCR. The graft survival was assessed by One-Way ANOVA and q test. Results Corneal allograft survival time: A (100.00±0.00), B ( 8.44±1.24), C (8.89±2.57), D (8.56±2.30), E (43.11±5.58), F (43.67±9.54), G (72.00±15.34)d. Group G led to a statistically significant prolongation of transplant survival and was superior than group E and F which was a statistical prolongation compared with group B, C and D (qGE=11.42, qGF=11.24,qEB=13.64, qEC=13.38, q<=13.46, qFB=13. 82, qFC=13.56, qFD=13.64; P<0.01). Immunohistopathologically, the grafts were subjected to an immune response contained a dense infiltrate of neutorphils,CD4+ and CD8+ T lymphocytes in the group B ,C and D. This cellular infiltrate was a significant reduction in group E,F,G. RT-PCR showed that the gene expression of IL-2 was inhibited earlier (3 days) in group F, G and VEGF gene expression being suppressed later (14 days) in group E, G. Conclusions Combined therapy with topical application of RAPA eye drop of nanometer vector and CsA PLA wafers can significantly prolong the survival of allograft at high-risk. Moreover, topical combined treatment of them is more effective, lower dosage, less side-effects and cheaper than the treatment with topical individual immunosuppressive drug.     

2型糖尿病患者血糖胰岛素及红细胞免疫功能的变化

目的 :探讨 2型糖尿病患者高水平血糖和胰岛素 (INS)对红细胞免疫功能的影响。方法 :选取我院已确诊的 74例 2型糖尿病患者 (T2 DM) ,分为高胰岛素血症 (2小时 INS≥ 82 m U/ L)组 2 9例和非高胰岛素血症(2小时 INS<82 m U/ L)组 4 5例 ,分别于空腹和口服葡萄糖后 12 0分钟时测定其血糖、INS、红细胞 C3b受体花环率 (RBC C3b RR)及红细胞免疫复合物花环率 (RBC ICR)变化 ,并设正常对照 32例。结果 :2型糖尿病患者高胰岛素血症组和非高胰岛素血症组与正常对照组相比 ,空腹的 RBC C3b RR均明显降低 (P均 <0 .0 1) ,RBC ICR均明显增高 (P均 <0 .0 1) ;高胰岛素血症组较非高胰岛素血症组 RBC C3b RR降低更为明显 (P均 <0 .0 1)。高胰岛素血症组、非高胰岛素血症组 RBC C3b RR在口服葡萄糖 12 0分钟时均较各自空腹时降低 (P均 <0 .0 1)。结论 :2型糖尿病患者红细胞 C3b受体活性降低与 INS和血糖水平升高有相关性。     

自身免疫性甲状腺疾病与Th1/Th2细胞因子

自身免疫性甲状腺疾病（AITD）的发生与Th1/Th2细胞群的偏移有关，桥本氏甲状腺炎的发病常表现为Th1细胞因子水平高于Th2细胞因子水平，而Graves'病中Th2细胞因子占优势。深入研究Th1/Th2细胞群的偏移在AITD发病中的作用，有助于进一步阐明AITD的发生机理。     

甲状腺激素对小鼠巨噬细胞生物活性影响的实验研究

目的 研究甲状腺激素对小鼠腹腔巨噬细胞的激活作用。方法 将三碘甲状腺原氨酸(T3)稀释为不同的水平作用于巨噬细胞，观察巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数，测定巨噬细胞中乳酸脱氢酶(LDH)和酸性磷酸酶(ACP)活性水平。结果 T3在0．75μg／L时，不仅提高巨噬细胞的吞噬率和吞噬指数，还可增加其LDH和ACP的活性；较高水平的T3对巨噬细胞的生物活性有一定的抑制作用。结论 甲状腺激素在一定的水平范围内，对小鼠腹腔巨噬细胞具有激活作用。     

泌乳素对诱发自身免疫性甲状腺炎影响的实验研究

目的 观察和研究泌乳索在诱发实验性自身免疫性甲状腺炎形成中的影响。方法 采用SD大鼠在溴隐亭的干预条件下，诱发实验性自身免疫性甲状腺炎(EAT)，观察大鼠甲状腺组织的病理改变和血清中Tg自身抗体、泌乳露水平及脾脏中的IL—2／IL—2RmRNA的表达。结果 大鼠经溴隐亭干预，诱发的实验性自身免疫性甲状腺炎发病率和甲状腺组织中的炎细胞浸润程度、炎症反应均较未经溴隐亭干预直接诱发EAT组(HI—EAT组)明显减轻，血清中Tg自身抗体平均水平明显高于未诱发EAT大鼠。结论 大鼠经溴隐亭干预，血清中泌乳素水平低下，致IL—2／IL—2RmRNA的表达减少，降低IL—2／L—2R系统对免疫应答的激活作用，其EAT的发病率和甲状腺组织中炎症反应得到缓解，高泌乳素血症也是自身免疫性疾病发病机制中的重要因素。