SNIP1在宫颈鳞状细胞癌中的表达及意义

目的:探究Smad核内相关蛋白1(Smad nuclear interacting protein 1,SNIP1)在正常宫颈上皮组织及宫颈鳞状细胞癌(宫颈鳞癌)组织中的表达及其意义.方法:免疫组织化学方法及实时荧光定量PCR(Real-time PCR)技术检测SNIP1在23例正常宫颈组织(正常宫颈组)、25例早期宫颈鳞癌组织(Ⅰ a～Ⅱa期)(早期宫颈鳞癌组)和45例晚期宫颈鳞癌组织(Ⅱb～Ⅲb期)(晚期宫颈鳞癌组)中的表达.结果:免疫组织化学方法显示SNIP1蛋白在正常宫颈组、早期宫颈鳞癌组及晚期宫颈鳞癌组中胞质强阳性表达率分别为0,36.0％和73.3％;胞核强阳性表达率分别为17.4％,44.0％和71.1％,且三组间胞质与胞核的表达差异均具有统计学意义(P＜0.01).在伴有淋巴结转移的宫颈鳞癌病例中,SNIP1在胞质、胞核中的强阳性率分别高于不伴淋巴结转移病例,且差异有统计学意义(P＜0.001).Real-time PCR技术检测显示SNIP1 mRNA在正常宫颈组、早期宫颈鳞癌组及晚期宫颈鳞癌组中表达呈递增趋势,除了正常宫颈组与早期宫颈鳞癌组间差异无统计学意义外,其余各组间差异有统计学意义(P＜0.05).结论:SNIP1可能参与调控宫颈鳞癌的进展及侵袭转移过程的发生,并有望成为宫颈鳞癌侵袭转移情况的预测因子.     

Neuropilin-1mRNA在不同动情周期及周龄阶段小鼠卵巢中的表达

目的:探讨Neuropilin-1(NRP -1)mRNA在繁殖适龄期小鼠动情周期间以及不同周龄阶段小鼠卵巢中的表达.方法:选用繁殖适龄期不同动情周期小鼠以及不同周龄阶段小鼠,通过实时定量聚合酶链反应检测其卵巢中NRP -1 mRNA的表达.结果:NRP -1在小鼠不同动情周期卵巢中的表达在mRNA水平差异无显著性(P ＞ 0.05).比较其mRNA在不同周龄阶段小鼠卵巢的中表达显示,近绝育期32周组表达水平显著高于其他组:为3周组的2.94倍(P ＜ 0.01),6周组的4.92倍(P ＜ 0.01),11周组的2.29倍(P ＜ 0.01).而其他各组间比较差异无统计学意义(P ＞ 0.05).结论:NRP -1 mRNA在小鼠卵巢中的表达不随动情周期而改变,且随着卵巢生理功能的下降而显著升高,提示NRP -1与卵巢衰老进程有关.     

吸烟与子宫内膜异位症发病风险的Meta分析

目的系统评价吸烟与子宫内膜异位症发病的相关性,为进行人群健康教育和预防决策提供依据。方法计算机检索Cochrane图书馆、PubMed、EMbase、CBM、CNKI和WanFang Data等数据库,查找吸烟与子宫内膜异位症发病风险的临床研究,由两名评价员独立按照纳入与排除标准选择文献、提取资料和评价质量后,对符合纳入标准的13个病例对照研究,采用RevMan 5.0软件进行Meta分析,计算其合并OR值和95%CI并对发表偏倚进行漏斗图分析。结果共纳入13个病例对照研究,共14 260例,其中子宫内膜症患者1 900例。方法学质量评价结果显示,2个研究为A级,4个为B级,剩余7个均为C级,总体研究质量不高。Meta分析结果显示,不吸烟人群与吸烟人群比较,其罹患子宫内膜异位症的几率未见增加[OR=0.91,95%CI(0.82,1.02)]。按地域进行亚组分析,结果显示北美洲人群的OR合并为0.96[95%CI(0.84,1.08)],吸烟组和非吸烟组子宫内膜异位症发病率差异无统计学意义,但欧洲人群的OR合并为0.72[95%CI(0.54,0.97)],吸烟组和非吸烟组子宫内膜异位症发病率差异有统计学意义。结论目前研究尚未见吸烟与否对子宫内膜异位症发病率有影响。由于各研究间存在异质性,且方法学质量较差,上述结论尚需开展更大样本量的前瞻性研究进一步证实。     

Suppression of EphB4 improves the inhibitory effect of mTOR shRNA on the biological behaviors of ovarian cancer cells by down-regulating Akt phosphorylation

The aim of the present study was to examine the effects of suppression of EphB4 and/or mTOR on the biological behaviors of ovarian cancer cells, and the potential regulatory pathways. An-tisense EphB4 vectors and shRNA vectors targeting mammalian target of rapamycin (mTOR) were constructed and transfected into A2780 and SKOV3 cells (two ovarian cancer cell lines). The effects of the antisense EphB4 vectors and the shRNA vectors on the proliferation, apoptosis and invasion of ovarian cancer cells were measured, and the expression of EphB4, mTOR and Akt detected. The results showed that transfection with mTOR shRNA could inhibit growth, induce apoptosis, and reduce invasive ability of ovarian cancer cells, which was accompanied by downregulation of EphB4, mTOR and Akt. The inhibitory effects on cell growth caused by mTOR shRNA alone were weaker than those by antisense pEGFP-C1-EphB4. In the antisense pEGFP-C1-EphB4-transfected cells, it was found that EphB4 knockdown could decrease the mTOR expression and slightly reduce the Akt phosphorylation. Significant suppressive effects on cell growth were observed in cells co-transfected with antisense pEGFP-C1-EphB4 and mTOR shRNA. In co-transfection group, the expression levels of EphB4, mTOR and Akt were distinctly lower than those in other groups. It was concluded that suppression of EphB4 may inhibit the growth of ovarian cancer cells by downregulation of the PI3K/Akt/mTOR pathway, and reverse Akt phosphorylation induced by mTOR shRNA. Inhibition of EphB4 and mTOR combined may cooperatively suppress the biological behaviors of ovarian cancer cells.     

中国五峰宫颈癌高发区妇女肿瘤防治知识调查

目的:了解宫颈癌高发区湖北省五峰自治县妇女肿瘤防治知识知晓情况。方法:采用随机抽样方法于2006～2008年对五峰县3个乡镇妇女肿瘤防治知识进行现场"一对一"问卷调查,共获得有效问卷5 936份。结果:被调查对象对我国妇女主要肿瘤及其症状知晓率较高;初中以上文化程度,年收入>3 500元,有肿瘤家族史的调查对象肿瘤防治知识知晓率高于对照组(P<0.05),不同年龄组间知晓率存在差异(P<0.05)。结论:湖北五峰县宫颈癌防治工作提高了当地妇女对肿瘤防治知识的认知,但仍需加大对低学历,低收入,无肿瘤家族史,高龄人群的宣传力度。     

妇科手术患者子宫内膜异位症发病因素分析

目的:探讨子宫内膜异位症在妇科手术患者中发病的相关危险因素。方法:选择99例行妇科手术证实为非内异症的良性疾病患者为对照组,选择95例子宫内膜异位症患者为病例组,对两组进行回顾性病例对照分析,对17个相关因素进行单因素、多因素非条件Logistic回归分析。结果:孕次、产次、人流次数、体重指数、职业、血型、避孕措施及原发性痛经为与子宫内膜异位症的发病相关的单因素(P<0.05),最终职业(OR=2.52,95%CI:1.28～4.94)、血型(OR=2.46,95%CI:1.13～5.36)、妊娠次数(OR=0.78,95%CI:0.65～0.93)、剖宫产史(OR=2.39,95%CI:1.07～5.38)、原发性痛经(OR=3.49,95%CI:1.81～6.73)5个因素进入多因素回归模型(P<0.05)。结论:从事脑力劳动、A型血、剖宫产史及原发性痛经为经手术证实子宫内膜异位症的主要危险因素,而妊娠次数多为保护因素。     

Risk factors for recurrence of ovarian endometriomas after surgical excision

Ovarian endometrioma is a common form of endometriosis, which may cause infertility, dysmenorrhea and pelvic pain in women of reproductive age. Although surgery is the treatment of choice for endometriomas, recurrence poses a formidable frustration. This study investigated potential risk factors of endometriomas recurrence, aiming to better understand its pathogenesis. A total of 307 patients with endometriomas were followed up for an average of 28.6 months and the 1-, 2- and 3-year cumulative recurrence rate was 9.5%, 21.9%, and 29.2%, respectively. Twenty-one potential risk factors for endometriomas recurrence were evaluated using Cox＇s proportional hazards models. Total revised American Fertility Society （rAFS） score was significantly associated with higher recurrence （OR=1.858, 95% CI=1.122-3.075, P=0.016）, as well as younger age at surgery （OR=0.953, 95% CI=0.915-0.992, P=0.020）. Semiradical surgical treatment was defined as surgical removal of cyst plus hysterectomy with preservation of bilateral or unilateral ovary, and was a significant factor that was associated with lower recurrence than the conservative surgery （OR=0.318, 95% CI=0.107-0.951, P=-0.040）. Postopera- tive pregnancy was favorable factors for disease recurrence （OR=0.217, 95% CI=0.102-0.460, P=0.000）. The results suggest that endometrioma recurrence is inversely associated with age at surgery and postooerative preanancv, and may correlate with total rAFS score and conservative surgery method.     

子宫颈癌疫苗制备的历史及现状

子宫颈癌是妇女最常见的恶性肿瘤之一,通过免疫途径寻找宫颈癌治疗的新突破是近二十年来子宫颈癌治疗的研究热点。即将上市的针对人乳头瘤病毒的子宫颈癌预防性4价疫苗,将宫颈癌免疫治疗正式纳入临床应用范畴。当前的子宫颈癌疫苗种类繁多,对这些疫苗的深入探讨将为子宫颈癌免疫治疗的进一步完善创造契机,同时为开发治疗性疫苗带来新思路。     

泰素加卡铂方案治疗晚期与复发性上皮性卵巢癌

为评价泰素加卡铂联合化疗治疗晚期与复发性上皮性卵巢癌的疗效，对23例采用泰素加卡铂治疗患者的疗效进行分析。14例初治晚期患者采用泰素加卡铂方案一线化疗，有效12例，完全缓解12例，2年无瘤存活者10例，9例复发患者采用泰素加卡铂方案二线化疗，有效4例，完全缓解2例，部分缓解2例，该方案主要副反应为骨髓抑制和关节肌肉痛，提示；泰素加卡铂方案可作为治疗晚期上皮性卵巢癌的首选一线方案。     

女性激素与认知功能相关性的研究进展

多项研究表明,女性激素与认知功能具有相关性。目前已发现雌激素、卵泡刺激素、黄体生成素和抗米勒管激素等女性激素及其受体在认知功能相关脑区如海马分布和表达,并对神经元或突触具有一定的调控作用。研究提示,孕激素和雄激素等与女性认知功能可能相关,但目前证据仍不够充分。本文旨在综述女性激素和认知功能的研究进展,有助于理解女性激素在认知功能发生发展中的作用,为阿尔茨海默病等认知功能障碍性疾病的治疗提供理论基础和研究思路