细胞色素芳香化酶P450在子宫内膜增生症及子宫内膜癌中的表达

目的探讨细胞色素芳香化酶P450在子宫内膜增生症与子宫内膜癌发病中的作用。方法应用免疫组织化学方法检测正常子宫内膜、子宫内膜增生症及子宫内膜癌中细胞色素芳香化酶P450的表达。结果细胞色素芳香化酶P450在正常子宫内膜无表达或表达弱,在子宫内膜增生症与子宫内膜癌表达增强。结论细胞色素芳香化酶P450促进子宫内膜增生症与子宫内膜癌的发生与发展,为选择细胞色素芳香化酶抑制剂治疗子宫内膜增生症和子宫内膜癌提供了理论依据。     

新城疫病毒D817对裸小鼠人结肠癌移植瘤的抑制作用

Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.     

新城疫病毒D817对裸小鼠人结肠癌移植瘤的抑制作用

Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.     

635名3～6岁儿童口腔健康行为与母亲文化素质关系的研究

目的:研究母亲的文化程度与学龄前儿童龋病和口腔健康行为的相关性。方法:对2004年1月～2005年3月来该院就诊的635名3～6岁初诊儿童按母亲的文化程度分为两组,并依据乳牙龋失补牙数(dmft)评价龋病状况。同时,就儿童的口腔健康行为向其母亲进行问卷调查。结果:两组儿童的龋均,两组儿童采用竖刷法、早晚刷牙和1～3个月更换牙刷等健康行为以及对龋病与食糖量关系的认识有显著性差异(P<0.05)。两组儿童对于一些口腔健康行为,龋病与食糖次数和龋病与饮用碳酸饮料密切的关系认识不足。结论:应加强学龄前儿童及其母亲的口腔健康教育,对文化程度较低的母亲要从孕期就开始宣传指导。加强宣传减少含糖食品摄入的次数,少喝或不喝碳酸饮料有益于降低龋病的发生率。     

米索前列醇与欣普贝生应用于胎膜早破促宫颈成熟的临床效果观察

目的：探析米索前列醇、欣普贝生(地诺前列酮栓）用于胎膜早破患者促宫颈成熟的临床效果,并对药物安全性进行分析。方法方便收集该院2013年8月一2014年8月间收治的90例胎膜早破宫颈不成熟的孕妇,将其分成观察组1、观察组2和对照组,每组30例,观察组给予米索前列醇,观察组2给予欣普贝生,对照组给予缩宫素促宫颈成熟,观察3组患者阴道分娩率、母胎感染率,并对3种药物用于促宫颈成熟的效果及安全性进行评价;为宫颈未成熟胎膜早破患者的临床治疗提供参考依据。结果应用3种药物治疗宫颈不成熟胎膜早破孕妇,观察组米索前列醇及欣普贝生的促宫颈成熟效果均为100%;两种药物的阴道分娩率分别为93.3%和90%,宫内感染率差异无统计学意义,临床治疗效果显著。结论米索前列醇及欣普贝生用于胎膜早破孕妇中的促宫颈成熟效果良好,患者阴道分娩率较高,宫内感染率低,与欣普贝生比较米索前列醇价格更优惠,口服小剂量米索前列醇促宫颈成熟效果好,阴道分娩率及安全性更高,可广泛应用于基层医院。     

维生素D3与不明原因稽留流产免疫学相关性的研究

目的探讨维生素D_3与不明原因稽留流产免疫学的相关性。方法选择2015-01~2016-03该院收治的不明原因稽留流产患者36例作为观察组,选择同期接受人工流产的正常产妇30例作为对照组。检测研究对象血清中的维生素D_3、γ-干扰素(IFN-γ)及白细胞介素-4(IL-4)水平,并对其相关性进行分析。结果观察组维生素D_3含量平均为(12.91±2.61)nmol/L,对照组为(20.03±3.82)nmol/L,组间比较差异具有统计学意义(P0.05);观察组IFN-γ明显高于对照组,而IL-4则显著低于对照组,差异具有统计学意义(P0.05);维生素D_3与IFN-γ间呈显著负相关,与IL-4呈显著正相关(P0.05)。结论维生素D_3水平异常可能会造成血清IFN-γ、IL-4水平异常,导致免疫功能异常,推断其与稽留流产的发生存在某种相关性。     

新型复合树脂Dyract应用的护理配合

Ｄｙｒａｃｔ属90年代新型复合材料,由底胶/粘接剂及单组份复合材料组成,经光敏固化。应用Ｄｙｒａｃｔ充填修复牙齿表面不需做预处理,材料有良好的粘接力,与牙齿间无微缝隙,密合度高。我院自1995年以来,应用该材料修复楔状缺损牙150颗,根面龋齿50颗,乳牙龋齿102颗,修复后...     

新城疫病毒D817对裸小鼠人结肠癌移植瘤的抑制作用

Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.     

诱导期血液透析患者健康教育的进展

血液透析是终末期肾衰竭患者重要的肾脏替代疗法之一.慢性肾衰患者由保守疗法向稳定的维持性血液透析治疗过渡的一段时期称为诱导透析期.诱导期透析的患者对血液透析知识的缺乏及血液透析中因机体内环境改变而产生的不良反应可加重患者对血液透析的恐惧和焦虑,同时由于体液量、电解质、酸碱平衡及尿毒症毒素等的突然变动,容易出现各类并发症,如失衡综合征、高血压、低血压、出血等.健康教育是通过信息传播和行为干预,帮助个人和群体掌握卫生健康知识,树立健康观念,自愿采纳有利健康的行为和生活方式的教育活动和过程[1],护理健康教育已经成为当前护理学专业最受瞩目的学科之一[2].     

喉罩联合右美托咪定在神经介入手术中的应用

目的比较不同剂量右美托咪定联合喉罩应用于神经介入手术的效果。方法 90例ASAⅠ、Ⅱ级在全身麻醉下行神经介入治疗颅内动脉瘤的患者随机分为3组:七氟醚和生理盐水组(对照组)、七氟醚和0.5μg/kg右美托咪定组(DEX1组)及七氟醚和1μg/kg右美托咪定组(DEX2组)。入选患者均采用丙泊酚和罗库溴铵全身麻醉诱导后,置入喉罩,术中维持七氟醚呼气末浓度为2 MAC。评价术后苏醒时间和躁动情况。结果 DEX2组术中心率减低(26.7%)发生率高于其他两组,DEX2组术后患者睁眼时间比其他两组延长近10 min。对照组术后躁动发生率较高,其他两组未见术后躁动。结论单次给予0.5μg/kg右美托咪定联合喉罩置入对神经介入治疗颅内动脉瘤患者术中的血液动力学影响较小,苏醒迅速,同时能够很好地预防术后躁动。