Structural basis of cargo recognitions for class V myosins

Class V myosins (MyoV), the most studied unconventional myosins, recognize numerous cargos mainly via the motor’s globular tail domain (GTD). Little is known regarding how MyoV-GTD recognizes such a diverse array of cargos specifically. Here, we solved the crystal structures of MyoVa-GTD in its apo-form and in complex with two distinct cargos, melanophilin and Rab interacting lysosomal protein-like 2. The apo-MyoVa-GTD structure indicates that most mutations found in patients with Griscelli syndrome, microvillus inclusion disease, or cancers or in “dilute” rodents likely impair the folding of GTD. The MyoVa-GTD/cargo complex structure reveals two distinct cargo-binding surfaces, one primarily via charge–charge interaction and the other mainly via hydrophobic interactions. Structural and biochemical analysis reveal the specific cargo-binding specificities of various isoforms of mammalian MyoV as well as very different cargo recognition mechanisms of MyoV between yeast and higher eukaryotes. The MyoVa-GTD structures resolved here provide a framework for future functional studies of vertebrate class V myosins.     

成都部队招待所从业人员乙肝病毒携带情况调查

目的 了解成都市部队招待所从业人员的乙肝病毒携带现状析,为乙肝防控提供依据.方法 统计分析2009-2011年间成都市部队招待所从业人员在成都军区疾病预防控制中心的体检结果.结果 该人群的乙肝携带率2009-2011年3年中最高为2.90％,远低于2011年全国人均携带率,乙肝携带率呈逐年下降趋势,有保护性抗体的人员逐年增加.结论 成都部队招待所的从业人员乙肝携带率较低,有保护性抗体人员逐年增加.     

重睑成形术前术后外眼形态的变化

目的：揭示重睑成形术后外眼形态变化的规律。方法：对９２例１８４只眼睛重睑成形术者术前术后眼裂高度和长度,两眼间距,眼球最突点位置,睫毛显露长度和上翘角度测量结果对比分析。结果：重睑成形术后眼裂高度,睫毛显露长度及睫毛上翘角度有显著性增大。结论：重睑成形术能使眼裂变大,睫毛全长暴露及睫毛上翘,从而增加眼睛的美感。     

负压吸引加碘伏冲洗对于糖尿病足创面生物膜的影响

目的：探讨生物膜在糖尿病足（diabetic foot,DF）患者创面愈合时的影响及初步的干预方法。方法：选取2016年8月至2018年8月住院接受负压吸引加碘伏冲洗治疗且资料完整的ＤＦ患者,对于治疗期间清创提取的分泌物标本进行细菌培养。选取部分病例,取创面基底组织进行革兰染色及阿辛蓝染色在光学显微镜下观察。取同一创面基底组织在透射电子显微镜下观察。所有数据录入SPSS 24.0统计软件进行统计分析。结果：细菌培养阳性结果的阳性率为58.3%,最常见的细菌为金黄色葡萄球菌、粪肠球菌和表皮葡萄球菌。在光学显微镜和透射电子显微镜下观察到细菌生物膜。通过负压吸引加碘伏冲洗治疗干预后,住院治疗30 d的愈合率为68.3%。ＤＦ治疗周期中位数为20 d,四分位间距（9,35） d。干预30 d后白细胞均值为（6.920±3.149）×109个/L。结论：ＤＦ创面感染时在光镜和透射电镜下证实疑似物质为细菌生物膜。负压吸引加碘伏冲洗治疗可以减少细菌生物膜在创面的生存机会。