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排序方式: 共有7124条查询结果,搜索用时 390 毫秒
121.
Akihito Sawada Yoshiaki Yamaji Tetsuo Nakayama 《Journal of infection and chemotherapy》2013,19(3):480-485
Aseptic meningitis and acute parotitis have been observed after mumps vaccination. Mumps outbreaks have been reported in Japan because of low vaccine coverage, and molecular differentiation is required to determine whether these cases are vaccine associated. RT-nested PCR was performed in the small hydrophobic gene region, and viruses were differentiated by restriction fragment length polymorphism assay. A total of 584 nucleotides were amplified. The PCR product of the Hoshino strain was cut into two fragments (313 and 271 nucleotides) by MfeI; that of the Torii strain was digested with EcoT22I, resulting in 332- and 252-nucleotide fragments. Both strains were genotype B and had an XbaI site, resulting in two fragments: 299 and 285 nucleotides. Current circulating wild types were cut only by XbaI or MfeI. However, the MfeI site of the wild types was different from that of the Hoshino strain, resulting in 451- and 133-nucleotide fragments. Using three restriction enzymes, two mumps vaccine strains were distinguished from wild types, and this separation was applied to the identification of vaccine-related adverse events. 相似文献
122.
Yoshiaki Katada Jun Isogai Hiroyasu Ina Mikio Tezuka Isao Umehara Hitoshi Shibuya 《Surgical and radiologic anatomy : SRA》2009,31(9):707-713
Purpose
Extraperitoneal spaces, such as the mesenteric space and the retroperitoneal space, can serve as areas that enable a reduction in the pressure exerted by extraperitoneal fluid collection and infiltrating diseases. In clinical practice, understanding the existence of these decompression spaces (or pathways) is very important for making accurate diagnoses. Here, we evaluated potential anatomical extraperitoneal spaces based on the extraluminal gas distribution in patients with pneumatosis intestinalis without intestinal ischemia. 相似文献123.
Yoshiyuki Kurata Machiko Oshida Hironori Take Takayasu Furubayashi Hirohisa Nakao Yoshiaki Tomiyama Yoshio Kanayama Nobuo Nagao Yasuto Okubo Takeshi Yonezawa and Seiichiro Tarui 《Vox sanguinis》1989,57(3):199-204
A new method was studied for eliminating HLA class I antigens from the surface of platelets without damaging the cells. Platelets were exposed to an acid solution (pH 3.0) to eliminate the antigenicity of HLA class I antigens. The reduction in antigenicities of HLA class I common antigen and individual HLA class I antigens by acid treatment was marked. Patients' sera which contained multispecific HLA antibodies reacted with PBS-treated platelets, but not with acid-treated platelets. No changes were observed in the antigenicities of glycoprotein Ib or glycoprotein IIb/IIIa. The viability of acid-treated platelets was 83%. Ultrastructural investigations revealed no significant difference between the PBS-treated platelets and acid-treated platelets. The platelet function studies showed that the aggregation of acid-treated platelets induced by various agonists was only slightly reduced compared with PBS-treated platelets. We propose that acid-treated platelets are promising for clinical use in patients refractory to platelet transfusions and may be superior to chloroquine-treated platelets for analysis of the specificity of antiplatelet antibodies. 相似文献
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Yao EH Fukuda N Ueno T Matsuda H Matsumoto K Nagase H Matsumoto Y Takasaka A Serie K Sugiyama H Sawamura T 《Hypertension》2008,52(1):86-92
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a membrane protein that can support the binding, internalization, and proteolytic degradation of oxidized low-density lipoprotein. The LOX-1 expression increases in the neointima after balloon injury. To develop an efficient compound to inhibit LOX-1, we designed and synthesized a novel gene silencer pyrrole-imidazole (PI) polyamide targeting the rat LOX-1 gene promoter (PI polyamide to LOX-1) to the activator protein-1 binding site. We examined the effects of PI polyamide to LOX-1 on the LOX-1 promoter activity, the expression of LOX-1 mRNA and protein, and neointimal hyperplasia of the rat carotid artery after balloon injury. PI polyamide to LOX-1 significantly inhibited the rat LOX-1 promoter activity and decreased the expression of LOX-1 mRNA and protein. After balloon injury of the arteries, PI polyamide to LOX-1 was incubated for 10 minutes. Fluorescein isothiocyanate-labeled PI polyamide was distributed to almost all of the nuclei in the injured artery. PI polyamide to LOX-1 (100 microg) significantly inhibited the neointimal thickening by 58%. PI polyamide preserved the re-endothelialization in the injured artery. PI polyamide significantly inhibited the expression of LOX-1, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, and matrix metalloproteinase-9 mRNAs in the injured artery. The synthetic PI polyamide to LOX-1 decreased the expression of LOX-1 and inhibited neointimal hyperplasia after arterial injury. This novel gene silencer PI polyamide to LOX-1 is, therefore, considered to be a feasible agent for the treatment of in-stent restenosis. 相似文献
127.
Burst patterns in the digastric, mylohyoid, and masseter muscles and the resultant jaw movement orbits during chewing and
swallowing were investigated in the freely behaving rabbit. Activities in the posterior mylohyoid fibers consisted of two
continuous bursts. Peaks in the first burst of the posterior fibers occurred in the middle part of opening and preceded the
digastric burst. Peaks in the second burst occurred in the final part of opening and coincided with those in the working side
of the digastric burst. After removal of the bilateral digastric muscles, the gape size during chewing was largely reduced
in the final part of opening and in the early part of closing. The results suggest that (a) the digastric may have a role
in opening the mandible widely beyond the rest position but may not have a major role in the control of the horizontal (mediolateral)
jaw movement, (b) the posterior mylohyoid fibers may have a function as an elevator of the tongue in the early part of opening,
and (c) the posterior mylohyoid fibers may have a function as a depressor of the jaw in the late part of opening. Electromyographic
burst in the mylohyoid muscle began with marked activity in the mid-closing phase. The results support a role for the mylohyoid
muscle as a leading muscle of swallowing. Swallowing events in the rabbit are easily distinguished from the activities of
the mylohyoid muscle and the thyrohyoid muscle. 相似文献
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Expression of GPIV and Naka antigen on monocytes in Naka -negative subjects whose platelets lack GPIV
Hironori Take Hirokazu Kashiwagi Yoshiaki Tomiyama Shigenori Honda Yumiko Honda Hajime Mizutani Takayasu Furubayashi Takahiro Karasuno Tetsuo Nishiura Yoshio Kanayama Yoshiyuki Kurata Yuji Matsuzawa 《British journal of haematology》1993,84(3):387-391
Summary. The platelet antigen Naka was once considered to be a platelet-specific alloantigen and is carried on platelet membrane glycoprotein (GP) IV. Recent studies suggest that Naka -negative subjects lack platelet GPIV. GPIV is an important adhesive receptor and expressed on the surface of monocytes as well as of platelets. In the present study, flow cytometry was used to detect GPIV and Naka antigen on the surface of monocytes. Naka antigen was expressed on monocytes as well as on platelets in Naka -positive subjects ( n = 6) (P-GPIV-positive subjects). To our surprise, monocytes of Naka -negative subjects ( n = 7) (P-GPIV-negative subjects) having no anti-Naka antibody in their serum expressed GPIV and Naka antigen to almost the same degree as did the monocytes of P-GPIV-positive subjects. Competitive experiments using OKM5 (a monoclonal antibody against GPIV) and anti-Naka antibody showed that the epitope of anti-Naka antibody on monocytes was very close to that of OKM5. In two P-GPIV-negative subjects having anti-Naka antibody in their serum, GPIV and Naka antigen were not expressed on the surface of either monocytes or platelets. These results indicate that the GPIV molecules and Naka antigen are expressed on the surface of monocytes in the majority of P-GPIV-negative subjects, but that in a very few P-GPIV-negative subjects neither GPIV nor Naka antigen is expressed on the surface of their monocytes. We hypothesize that P-GPIV-negative subjects who carry neither GPIV nor Naka antigen on their monocytes produce anti-Naka antibody as a result of transfusion or pregnancy. 相似文献