The distribution of nitric oxide synthetase in Vcx and its relation with the expression of FOS induced by teeth movement in rats]

It was studied the central role of nitric oxide(NO) during experimental teeth movement and the relation between nitric oxide synthetase (NOS) positive neurons and FOS like immunoreactivity (FLN) with the NADPH-diaphorase histochemistry and immunocytochemical reaction method. Results indicated that NOS positive neurons and FLN showed typical distribution in Vcx and there was some overlap between them. It suggests that NO is involved in the central modulation of the stimulating message of teeth movement, and which further explains the central modulation mechanism of experimental teeth movement in rats.     

Type II collagen and aggrecan mRNA expressions in rabbit condyle following disc displacement

The aim of this investigation was to study the remodelling of cartilage in the mandibular condyle following disc displacement (DD) of the temporomandibular joint (TMJ). Forty adult Japanese white rabbits were used in this study. The right joints of 28 of the 40 rabbits had their discs surgically displaced. Four of the 28 were killed at 4 days or 1, 2, 4, 6, 8 and 12 weeks after surgery. The messenger RNA (mRNA) expression levels of aggrecan and type II collagen in cartilages were measured using in situ hybridization techniques. Results showed that aggrecan mRNA expression reduced in the first week after DD. The expression began to recover after 4 weeks and reached a normal level after 6 weeks. Type II collagen mRNA expression reduced from 4 weeks and the expression recovered after 8 weeks. This suggests that the chondrocyte reacting to the displacement of the TMJ disc, alters its matrix gene expression patterns and it is may be the cause of the shape changes of TMJ after DD.     

倾斜种植体在上颌后牙区骨量不足患者中的应用

目的:探讨倾斜种植体作为一种避免上颌窦植骨的方法,应用于上颌骨后牙区骨量不足患者种植修复的可靠性。方法:2005年1月—2007年12月间,21例患者共27个固定桥修复上颌后牙缺失,分别使用ITI和Br覽nemark 2种种植系统共植入78颗种植体,其中34颗种植体采用倾斜植入,44颗轴向植入。所有患者均为上颌后牙区骨量不足无法直接接受常规种植体植入。种植体植入后常规愈合3个月,除1颗种植体失败外,其余种植体均功能性负载支持固定义齿修复,修复方式为黏结固位和螺丝固位。每例患者均于负载后12、24和36个月接受临床和放射学随访检查。采用SPSS11.0软件包对数据进行统计学处理。结果:上颌有1颗轴向种植体在术后2个月时失败,倾斜种植体无失败。随访时间为36个月。上颌轴向种植体的累积存活率为97.72%,倾斜种植体的累积存活率为100%,修复成功率为100%。随访期间,种植体周围软组织保持稳定,平均探诊深度和附着水平无明显变化。结论:倾斜种植体作为一种上颌骨后牙区骨量不足患者的治疗方法是可靠的,能有效避免植骨手术,并节约治疗时间。     

64排螺旋CT灌注诊断涎腺肿瘤的临床应用研究

目的:评价64排螺旋CT灌注成像对涎腺肿瘤的临床诊断价值。方法:29例涎腺部肿瘤(腮腺22例,颌下腺6例,舌下腺1例;其中良性肿瘤17例,恶性肿瘤12例)行CT灌注检查,取得相应的灌注参数:血流量(blood flow,BF),血容量(blood volume,BV),平均通过时间(mean transit time,MTT)和表面渗透性(permeability surface,PS),并均经术后病理证实。结果:所有肿瘤的BF、BV、MTT和PS值明显高于正常涎腺组织(P<0.05),恶性肿瘤组BF、BV、PS值高于良性肿瘤组(P<0.05),MTT参数则在良、恶性肿瘤之间无统计学差异。结论:涎腺肿瘤的CT灌注成像是一种较为准确的定量分析肿瘤血流灌注状态的方法,具有较高的临床应用价值。     

锥形束CT在治疗上颌前牙区埋伏牙中的应用

目的:探讨锥形束CT(CBCT)在治疗上颌前牙区埋伏牙中的临床应用价值。方法:收集我院2009—2010年经普通X线片确定有埋伏牙,但不易具体定位的患者11例,进行CBCT扫描和三维重建,确定埋伏牙在颌骨内的具体位置。结果:经CBCT扫描、三维重建后,所有患者均可直观再现埋伏牙的形态、数目、在颌骨中的具体位置和发育情况。11例患者共16颗埋伏牙,其中既有埋伏阻生牙又有埋伏多生牙,3例(4颗)为埋伏多生牙,1例有2颗埋伏牙;2例为倒置埋伏,2例为水平埋伏,3例为垂直埋伏,4例为斜位埋伏。根据CBCT结果制订治疗方案,获得满意的治疗效果。结论:CBCT三维重建对于上颌前牙区埋伏牙治疗方案的设计及实施具有重要意义。     

Reliability of quantitative laser fluorescence analysis of smooth surface lesions adjacent to the gingival tissues

The aim of this study was to determine the impact of analysis patch border inclusion or exclusion on the reliability of quantitative laser fluorescence (QLF) analyses of lesions close to the gingival margin. Ninety-three lesions on the maxillary anterior teeth were imaged using a fluorescent capturing system. All the lesions were located on the gingival third of the teeth. One examiner undertook two analyses of the images 1 week apart and another examiner undertook a single analysis. Analyses were undertaken using QLF 2.00 g with four patch borders. Each border was assessed as active or inactive. QLF metrics DeltaF, DeltaQ and area were exported. Kappa statistics were used to measure the agreement of border inclusion between all three analyses, and intra-class correlation coefficients (ICCs) were used to determine the intra- and inter-examiner reliability of the QLF metrics. Agreement on border exclusion was poor; with intra-examiner kappa of 0.48 and inter-examiner at 0.20. However, despite the inconsistencies in border exclusions the ICCs for each QLF metric were high; intra-examiner DeltaQ 0.91, DeltaF 0.80 and area 0.92; inter-examiner DeltaQ 0.86, DeltaF 0.68, area 0.88. Lesions adjacent to the gingival margin will often require a patch analysis border to be excluded in order to ensure a satisfactory reconstruction and thus accurate analysis. The decision to include or exclude a border and, if excluded, which border to select appears to be highly variable between examiners. Nevertheless, the QLF metrics appear to be robust as demonstrated by the high ICCs noted in this study.     

含氟封闭剂三年临床防龋效果观察

该研究主要观察含氟封闭剂扔临床防龋效果，并将它同其它几种防龋方法进行了比较。作者将上海市７８６名６－８岁儿童分成四组，分别作四种处理，它们是；含氟封闭剂，普通封闭剂，ＡＰＦＩ凝胶，普通封闭剂加ＡＰＦＩ凝胶。封闭剂在研究开始时使用于第一恒磨牙，ＡＰＦＩ凝胶每半年使用一次。３年后观察这四种防龋方法对儿童牙齿的保护作用，结果显示１；含氟封闭剂对受封闭牙面的防龋效果较普通封闭剂和ＡＰＦＩ凝胶为高；对口腔中     

Novel identification of a four-base-pair deletion mutation in PITX2 in a Rieger syndrome family

Rieger syndrome is one of the most serious causes of tooth agenesis. Mutations in the PITX2, FOXC1, and PAX6 genes have been associated with Rieger syndrome. We have studied a three-generation Chinese family affected with Rieger syndrome and showing prominent dental abnormalities. Mutational screening and sequence analysis of the PITX2 gene revealed a previously unidentified four-base-pair deletion of nucleotides 717-720 in exon 5 in all affected members. The mutation causes a frame shift after Thr44, the 7th amino acid of the homeo-domain, and introduces a premature stop codon in the gene sequence. This deletion is the first unquestionable loss-of-function mutation, deleting all the functionally important parts of the protein. Our novel discovery indicates that the oligodontia and other phenotypes of Rieger syndrome observed in this family are due to this PITX2 mutation, and these data further support the critical role of PIXT2 in tooth morphogenesis.     

义齿稳固剂的研究进展

义齿稳固剂作为辅助活动义齿固位及稳定的有效制剂,在患者中的认知率仍不足.本文就义齿稳固剂的研究进展进行综述,指出义齿稳固剂可以增加义齿稳定性和适合性.