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Mulyanto Sulaiman Ngongu Depamede Made Sriasih Masaharu Takahashi Shigeo Nagashima Suljid Jirintai Tsutomu Nishizawa Hiroaki Okamoto 《Archives of virology》2013,158(1):87-96
One hundred sixteen rats (Rattus rattus) captured in Indonesia from 2011 to 2012 were investigated for the prevalence of hepatitis E virus (HEV)-specific antibodies and HEV RNA. Using an ELISA based on HEV genotype 4 with an ad hoc cutoff value of 0.500, 18.1 % of the rats tested positive for anti-HEV IgG. By nested RT-PCR, 14.7 % of the rats had rat HEV RNA, and none were positive for HEV genotype 1-4. A high HEV prevalence among rats was associated with lower sanitary conditions in areas with a high population density. Sixteen of the 17 HEV isolates obtained from infected rats showed >93.0 % nucleotide sequence identity within the 840-nucleotide ORF1-ORF2 sequence and were most closely related to a Vietnamese strain (85.9-87.9 % identity), while the remaining isolate differed from known rat HEV strains by 18.8-23.3 % and may belong to a novel lineage of rat HEV. These results suggest a wide distribution of rat HEV with divergent genomes. 相似文献
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Noninvasive investigation of exocrine pancreatic function: Feasibility of cine dynamic MRCP with a spatially selective inversion‐recovery pulse 下载免费PDF全文
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Kurashima K Kanauchi T Takayanagi N Sato N Tokunaga D Ubukata M Yanagisawa T Sugita Y Kanazawa M 《Respirology (Carlton, Vic.)》2005,10(5):572-578
OBJECTIVE: Chronic Chlamydia pneumoniae infection has been identified serologically in patients with COPD. The aim of this study was to examine whether the severity of emphysema is related to elevated antibody titres against C. pneumoniae. METHODOLOGY: We measured antibody titres against C. pneumoniae using ELISA, and assessed the severity of emphysema by the percentage of low attenuation area (%LAA) using high resolution (HR) CT in patients with COPD and in non-smoking control subjects. RESULTS: The mean %LAA was 2.2% in non-smoking controls (n = 28) and 13.3% in COPD patients (n = 94). COPD patients with a high IgG antibody index to C. pneumoniae (> or =2.0, n = 42) had a significantly higher %LAA (16.8%) than those with a low IgG index (<2.0, n = 52) (10.6%, P = 0.01). In addition, COPD patients with a high IgA antibody index (> or =2.0, n = 46) had a significantly higher %LAA (15.9%) than those with a low IgA index (<2.0, n = 48) (10.9%, P = 0.048). COPD patients with a high IgA antibody index also had a significantly lower %DLco than that associated with a low IgA index (68.1% and 80.3%, respectively, P = 0.007). There were no significant differences in age, smoking index or FEV(1)/FVC between these groups. CONCLUSION: These results suggest that high antibody titres against C. pneumoniae are linked with the severity of emphysema on high resolution CT and decreased diffusing capacity to carbon monoxide. 相似文献
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Right-lobe living donor liver transplantation (RL-LDLT) has become an acceptable procedure for adult patients with end-stage
liver disease in this decade. However, biliary complications in RL-LDLT remain a serious problem: the incidence of anastomotic
biliary leakage and stricture after RL-LDLT is reported to be 4.7%–18.2% and 8.3%–31.7%, respectively. The incidence varies
according to the type of biliary reconstructions between Roux-en-Y hepaticojejunostomy and duct-to-duct biliary reconstruction.
The anatomical biliary diversity of a right-lobe graft makes it difficult to reconstruct the biliary system. Indeed, most
biliary strictures in patients with duct-to-duct reconstruction develop in multibranched fashion. In this regard, endoscopic
biliary stenting appears to be efficacious for treating multibranched biliary strictures because multiple stenting permits
the drainage of each segmental branch of the stricture. In this review, we describe various aspects of biliary complications
occurring in RL-LDLT and their treatment. 相似文献