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排序方式: 共有226条查询结果,搜索用时 15 毫秒
91.
We report a de novo heterozygous 5,013,940 bp terminal deletion of chromosome 15q26 in a 13 9/12 -year-old Japanese girl with short stature (-3.9 SD), mild mental retardation, and ventricular septal defect (VSD). This terminal deletion involved IGF1R but not NR2F2, and was associated with an addition of telomere repeat sequences (TTAGGG) at the end of the truncated chromosome. The results provide further support for the notion that terminal deletions are healed by de novo addition of telomere sequences essential for chromosome stability and DNA replication. Furthermore, while growth failure and mental retardation are primarily explained by loss of IGF1R, the occurrence of VSD might suggest the existence of a cardiac anomaly gene, other than the candidate cardiac anomaly gene NR2F2, in the deleted region. 相似文献
92.
93.
Katayama R Watanabe K Yamagishi N Abe S Satoh H Furuhama K 《Journal of applied toxicology : JAT》2011,31(4):360-365
To estimate the glomerular filtration rate (GFR) sequentially in conscious rats, we validated a single-blood-sample method using a bolus injection of the nonionic contrast medium iodixanol as a tracer. First, to clarify basal age-related GFR profiles, iodixanol was intravenously administered once weekly at 1500 mg kg(-1) I to clinically healthy male F344 rats from 6 to 15 weeks of age. The blood sample was collected 120 min later, and serum iodixanol concentration was measured by HPLC. GFR values decreased gradually by 9 weeks, presumably due to rapidly increased body weights, and then remained constant from 10 weeks onward. When converting the GFR from body weight to body surface area, the reference range (40-60 ml min(-1) m(-2) ) in the latter was much more stable than that (6-11 ml min(-1) kg(-1) ) in the former. For nephropathy rats induced by cisplatin (3.75-7.5 mg kg(-1) , i.v., single dose), bromoethylamine hydrobromide (BEA, 250-500 mg kg(-1) , i.v., single dose) or puromycin aminonucleoside (PAN, 15 mg kg(-1) day(-1) , s.c., 10 days), GFR decreased or tended to decrease before increasing in serum urea nitrogen (UN) and creatinine concentrations. Accordingly, serum UN and creatinine concentrations became elevated when the GFR decreased to 50-60% of the basal value. This method without urine collection contributes to the reduction of animal numbers because of repeated application to the same animals. 相似文献
94.
Sumito Sato Charlotte Kopitz Bettina Grismayer Nathalie Beaufort Ute Reuning Manfred Schmitt Thomas Luther Matthias Kotzsch Achim Krüger Viktor Magdolen 《Breast cancer research and treatment》2011,127(3):649-657
uPAR, the three-domain membrane receptor of the serine protease urokinase, plays a crucial role in tumor growth and metastasis.
Several mRNA splice variants of this receptor have been reported. One of these, uPAR-del4/5, lacking exons 4 and 5, and thus
encoding a uPAR form lacking domain DII, is specifically overexpressed in breast cancer and represents a statistically independent
prognostic factor for distant metastasis-free survival in breast cancer patients. The aim of the present study was to examine
the molecular and cellular properties of the encoded uPAR-del4/5 protein. To investigate the impact of the uPAR-del4/5 overexpression
on in vitro and in vivo aspects of tumor progression (e.g., proliferation, adhesion, invasion, metastatic seeding, and/or
metastatic growth), we combined the analysis of transfected cancer cell lines with a murine xenograft tumor model. Increased
expression of uPAR-del4/5 in human cancer cells led to reduced adhesion to several extracellular matrix proteins and decreased
invasion through MatrigelTM, while cell proliferation was not affected in vitro. Moreover, invasion of uPAR-del4/5 overexpressing cells was not altered
by addition of urokinase, while that of uPAR-wild-type overexpressing cells was drastically increased. Accordingly, we observed
that, in contrast to uPAR-wild-type, uPAR-del4/5 does not interact with urokinase. On the other hand, when overexpressed in
human breast cancer cells, uPAR-del4/5 distinctly impaired metastatic dissemination and growth in vivo. We demonstrate that
the uPAR-del4/5 mRNA splice variant mediates tumor-relevant biological processes in vitro and in vivo. Our results thus illustrate
how tumor-specific alternative splicing can distinctly impact the biology of the tumor. 相似文献
95.
Intronic single-nucleotide polymorphisms in Bcl-2 are associated with chronic obstructive pulmonary disease severity 总被引:1,自引:0,他引:1
Sata M Takabatake N Inoue S Shibata Y Abe S Machiya J Wada T Ji G Kido T Matsuura T Muramatsu MA Kubota I 《Respirology (Carlton, Vic.)》2007,12(1):34-41
BACKGROUND AND OBJECTIVE: COPD is a multifactorial disease influenced by genetic and environmental factors, and gene-by-environmental interactions. There is considerable variability in the degree of airflow obstruction, moreover only 10-15% of chronic smokers develop COPD. These observations indicate that additional risk factors, possibly genetic, contribute to not only the susceptibility to COPD but also the development and severity of COPD. Recent paradigms highlight the presence and causal role of apoptosis in emphysema. There is a large amount of information on the genes involved in the regulation of apoptosis and one of the most studied is Bcl-2. The aim of this study was to investigate the genetic association of Bcl-2 gene with the level of lung function, that is, the severity, of COPD. METHODS: The genetic association of Bcl-2 polymorphisms with lung function was investigated in 261 Japanese patients with COPD using 12 single-nucleotide polymorphisms (SNPs) in Bcl-2. RESULTS: Four SNPs showed a significant association between the high and low lung function groups in a dominant trait comparison. Subsequent linkage-disequilibrium mapping and analyses of haplotype structure also showed a significant association between the level of lung function and two haplotypes comprised of the associated SNPs in Bcl-2. CONCLUSIONS: Although the linkage between Bcl-2 gene and the susceptibility to COPD remains to be clarified, the findings of the current study indicate that Bcl-2 might be influencing the level of lung function, that is, the development and severity of COPD. 相似文献
96.
97.
Watanabe I Nagao K Tani S Masuda N Yahata T Ohguchi S Kanmatsuse K Kushiro T 《Heart and vessels》2006,21(4):236-241
The increasing elderly population will influence the treatment policies adopted in cases of acute myocardial infarction. Considering
reperfusion therapy in elderly patients with acute myocardial infarction, we compared three strategies, as follows: primary
percutaneous coronary intervention (primary PCI: n = 26), facilitated PCI with half the standard dose of mutant tissue-type plasminogen activator (t-PA) (half + PCI: n = 24), and facilitated PCI with a standard dose of mutant t-PA (standard + PCI: n = 15) between patients 75 and 80 years of age. The rate of acquisition of thrombolysis in myocardial infarction (TIMI-3)
flow on initial coronary arteriography was significantly lower in the primary PCI group than in the other two groups (7.7%
in the primary PCI group vs 60% in the half + PCI and 66.7% in the standard + PCI group). The incidence of hemorrhagic complications
including blood transfusion was not significantly different between primary PCI and facilitated PCI. Considering reperfusion
therapy in elderly patients with acute myocardial infarction, we concluded that facilitated PCI may be effective in elderly
patients aged 75–80 years. 相似文献
98.
Yukihiro Minegishi Sumito Inoue Kento Sato Koya Abe Hiroaki Murano Kodai Furuyama Sujeong Yang Hiroyoshi Machida Hiroshi Nakano Masamichi Sato Takako Nemoto Chisa Sato Michiko Nishiwaki Tomomi Kimura Keiko Yamauchi Akira Igarashi Yoshikane Tokairin Yoko Shibata Masafumi Watanabe 《Respiratory investigation》2019,57(2):191-197
Background
Elderly patients who are hospitalized due to pneumonia experience deterioration of their activities of daily living (ADL) during this period; in some cases, this loss of ADL is not recovered at the end of antibiotic treatment. In this study, we examined whether erector spinae muscle cross-sectional area (ESMCSA) measured by computed tomography (CT) could predict a low level of ADL at the end of antibiotic treatment for pneumonia.Methods
Eighty patients (mean age 74.8 years) with pneumonia, who were admitted to Yamagata university hospital between 2015 and 2016, were analyzed retrospectively. In all cases, chest CT was performed on admission and ESMCSA was measured at the level of the 12th thoracic vertebra. Patient levels of ADL were also measured, both on admission and at the end of treatment, using the Barthel Index.Results
Patients with lower levels of ADL at the end of treatment were significantly older and tended to have a lower body mass index, poorer nutritional status, and more severe pneumonia than did patients who were self-reliant. Significantly smaller ESMCSAs were noted in patients who required assistance at the end of treatment than in those who were self-reliant. In multivariate logistic regression analysis, smaller ESMCSA was significantly associated with a lower level of ADL at the end of treatment, independent of age, sex, severity of pneumonia, nutritional status, or dehydration status.Conclusion
These results suggest that ESMCSA can predict ADL level after antibiotic treatment of pneumonia. 相似文献99.
Kamihira S Dateki N Sugahara K Yamada Y Tomonaga M Maeda T Tahara M 《International journal of hematology》2000,72(1):79-84
We describe the establishment of a real-time polymerase chain reaction (PCR) assay for the quantitative estimation of human T-cell leukemia virus type 1 (HTLV-1) proviral load using a LightCycler Technology (Roche Diagnostics, Mannheim, Germany) instrument. Proviral DNA level represents a measure of the integrated viral genome in host cells, so we applied this technique to evaluate the tumor burden in adult T-cell leukemia (ATL) patients with aberrant integration patterns of HTLV-1 detected by standard Southern blot hybridization (SBH) analysis. In 14 of our 15 ATL cases with 2 or more bands detected by SBH analysis, the ATL cells were shown to harbor multiple copies of the provirus within 1 ATL cell. This result suggests the usefulness of real-time PCR quantification for the study of the relationship between ATL pathology and HTLV-1-induced pathogenesis. 相似文献
100.
Dimayuga P Cercek B Oguchi S Fredrikson GN Yano J Shah PK Jovinge S Nilsson J 《Arteriosclerosis, thrombosis, and vascular biology》2002,22(4):644-649
We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020+/-0.0160 [n=8] versus 0.0049+/-0.0022 [n=8] mm(2), respectively; P<0.05) and in western-type diet-fed Rag-1 KO mice compared with WT mice (0.0312+/-0.0174 [n=7] versus 0.0050+/-0.0028 [n=6] mm(2), respectively; P<0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076+/-0.0039 [n=9] versus 0.020+/-0.0160 [n=8] mm(2), respectively; P<0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087+/-0.0037 [n=8] versus 0.0312+/-0.0174 [n=7] mm(2), respectively; P<0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury. 相似文献