微波消融联合载阿霉素微泡靶向击破在小鼠H22肝癌治疗中的效果观察

【摘要】 目的 观察微波消融（MWA）联合载阿霉素微泡靶向击破治疗小鼠H22肝癌皮下瘤的效果。 方法 制备空白微泡和载阿霉素微泡,检测其理化特性。流式细胞仪分析不同给药方式下体外细胞内药物浓度。构建BALB/c小鼠H22肝癌皮下瘤模型,将72只荷瘤小鼠随机均分为单纯MWA组（A组）、MWA+阿霉素组（B组）、MWA+空白微泡组（C组）、MWA+载阿霉素微泡组（D组）,其中C、D组微泡经低频超声击破。记录小鼠肿瘤体积变化,构建Kaplan-Meier生存曲线,比较心、肾组织内药物浓度。病理切片检测各组残存活性肿瘤区微血管密度（MVD）和Ki-67表达,并进行统计学分析。结果 体外细胞实验显示,低频超声靶向击破微泡技术能显著提高肿瘤细胞内药物浓度（P=0.011）;体内实验显示,D组抑制肿瘤生长显著优于A组（P=0.008 5）,B组、D组小鼠生存时间均比A组显著延长（P=0.009,P=0.003）,同时D组心、肾组织内药物浓度均显著降低（P=0.012,P=0.045）。D组残存活性肿瘤区MVD与A组相比显著降低（P＜0.000 1）,B、D组肿瘤细胞Ki-67表达均显著降低（P＜0.001）。结论 MWA联合载阿霉素微泡超声靶向击破可提高肝癌细胞内药物浓度,抑制肿瘤增殖和微血管生成,弥补单纯MWA治疗适形性不足,同时降低药物心、肾不良反应。     

Molecular variability of sweet potato chlorotic stunt virus (SPCSV) and five potyviruses infecting sweet potato in China

To obtain a better understanding of the molecular variation of sweet potato viruses in China, 131 samples were collected from symptomatic sweet potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of sweet potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect sweet potato in China, and three different strains of SPFMV and two of SPVG were found.     

Religious service attendance typologies and African American substance use: a longitudinal study of the protective effects among young adult men and women

Purpose

This study sought to identify variation by gender in the associations between religious service attendance from adolescence to young adulthood and seven measures of lifetime and short-term substance use.

Methods

To conduct this nationally representative study, data from the Add Health Surveys was abstracted from Waves I and IV (N = 3,223) to construct four types of service attendance (non-attenders, attenders only as adolescents, attenders only in young adulthood, and consistent attenders). A series of logistic regressions were conducted to identify the independent effects of each pattern of service attendance on each substance among all black young adults, as well as male and female sub-samples.

Results

Analysis revealed consistent attenders were generally less likely to use substances, with the effects being strongest among females. Among young adult only attenders, males recorded lower odds across all three short-term measures whereas females reported lower odds only for monthly cigarette use.

Conclusion

The protective effects of religious service attendance are more robust for African Americans who consistently attend in adolescence and young adulthood, especially among females.

     

Lyssavirus surveillance in bats of southern China’s Guangxi Province

Although rabies virus is widely distributed in the world, and has been the subject of extensive investigations with the objective of its ultimate prevention, control, and management, there is much less knowledge of the characteristics, distribution, and infectivity of other lyssaviruses. Since bats are known animal vectors for all but one of the known lyssavirus genotypes, we have performed an extensive survey of bats in the Guangxi Province to provide information on lyssavirus distribution in southern China. The lyssavirus nucleoprotein gene was detected in brains of 2.86 % of 2,969 bats. Nucleotide sequence homologies among isolates were 86.9–99.6 %, but only 70.0–85.0 % for lyssaviruses in GenBank. These infected bats were detected from a wide area, essentially forming a band running from the south-west to the north-east of Guangxi, and it appears that infection by new lyssaviruses is widespread in this region.     

丹参酮ⅡA磺酸钠对胰腺癌BX-PC-3细胞系增殖与细胞周期调控因子表达的影响

 目的：观察丹参酮ⅡA磺酸钠对胰腺癌细胞系BX-PC-3细胞增殖和细胞周期调控因子cyclin A、cyclin D2蛋白表达的影响。方法：分别用不同剂量的丹参酮ⅡA磺酸钠作用于胰腺癌细胞系BX-PC-3,应用MTT比色法检测培养48 h的细胞存活率, 应用流式细胞术测定培养48 h细胞周期的变化,Western blotting检测周期蛋白cyclin A和cyclin D2 的表达情况。结果：丹参酮ⅡA磺酸钠能明显抑制BX-PC-3细胞增殖,且具有明显的剂量依赖性;流式细胞术显示一定浓度药物作用细胞,可将细胞周期阻滞于G0/G1期;Western blotting检测发现药物能显著下调周期蛋白cyclin A和cyclin D2的表达水平。结论：丹参酮ⅡA磺酸钠能显著抑制人胰腺癌BX-PC-3细胞增殖,下调周期相关因子cyclin A和cyclin D2蛋白表达水平,这可能是丹参酮ⅡA磺酸钠抑制胰腺癌细胞生长增殖的作用机制。     

精神分裂症患者与分裂型人格个体的时间加工行为特点

目的:探讨精神分裂症患者与分裂型人格个体的时间加工行为特征.方法:选取59名符合美国精神障碍诊断与统计手册第4版(DSM-Ⅳ)精神分裂症诊断标准的门诊或住院患者,62名通过分裂型人格问卷(SPQ)筛查的分裂型人格个体以及60名健康对照人群.通过时间辨别任务、时间产生任务、时间复制任务对3组被试的时间加工能力进行测查.结果:时间产生任务中,分裂型人格组的准确性得分大于健康对照组[(1.03±0.07) vs.(0.99±0.09),P＜0.05],精神分裂症组的变异系数大于健康对照组和分裂型人格组[(0.26±0.10) vs.(0.20±0.05),(0.20±0.05);均P＜0.01];时间复制任务中,精神分裂症组的变异系数大于健康对照组和分裂型人格组[(0.21±0.11) vs.(0.15±0.07),(0.17±0.12);均P＜0.01).结论:精神分裂症患者和分裂型人格个体都存在时间加工表现上的缺损,其中精神分裂症患者的缺损可能更为严重.     

海军某部官兵的自杀意念及相关因素

目的:调查海军官兵自杀意念的流行现状并初步探讨其相关因素。方法:选取海军某部官兵1710人,完成自编人口学调查表、抑郁焦虑压力自评量表中文简体版(DASS-C21)、艾森克人格问卷简式量表中国版(EPQ-RSC)和自杀意念自评量表(SIOSS)。结果:本样本1年自杀意念检出率为3.56%。多因素logistic回归分析显示,与父母关系一般和差、经常和偶尔出现口腔溃疡症状、重度和极重度抑郁、重度和极重度压力、高神经质、高精神质是自杀意念的危险因素(OR=2.74、3.48、10.77、3.54、51.80、.6.40)。结论:与父母关系、口腔溃疡症状、抑郁、压力和人格特质是与海军官兵自杀意念相关的危险因素。提示在海军官兵自杀预防中,除传统的心理和社会学因素外,也应重视官兵身体健康异常的问题。     

CYP2C19、CYP3A5基因多态性与心肌梗死的相关性研究

目的探讨CYP2C19、CYP3A5基因的多态性与心肌梗死发病风险的相关性。方法随机选取心肌梗死患者及健康对照各500例,采用荧光PCR法和Sanger测序分别检测其CYP2C19、CYP3A5基因的多态性,用Logistic回归分析其与心肌梗死的相关性,用Quanto软件评估统计学效能。结果CYP2C19基因rs4986893位点的AG、GG基因型和A等位基因的频率以及CYP3A5基因rs776746位点的AA、AG、GG基因型和G等位基因频率在两组之间的差异具有统计学意义(P<0.05),CYP2C19基因rs4244285、rs12248560位点的基因型和等位基因以及rs4986893位点的AA基因型的频率在两组之间差异无统计学意义(P>0.05)。在校正年龄、性别、体质指数后,Logistic回归分析显示CYP2C19基因rs4986893的AG基因型和A等位基因以及CYP3A5基因rs776746的GG基因型和G等位基因可能是心肌梗死发病的风险因素,而rs4986893的GG基因型以及rs776746的AA、AG基因型可能是心肌梗死的保护因素。依据样本量、样本结构和等位基因频率以及Quanto分析,本研究的结果具有理想的统计学效能(99%)。结论CYP2C19、CYP3A5基因的多态性可能增加心肌梗死的发病风险。