全文获取类型
收费全文 | 2177篇 |
免费 | 116篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 99篇 |
妇产科学 | 73篇 |
基础医学 | 177篇 |
口腔科学 | 192篇 |
临床医学 | 132篇 |
内科学 | 726篇 |
皮肤病学 | 113篇 |
神经病学 | 146篇 |
特种医学 | 49篇 |
外科学 | 247篇 |
综合类 | 45篇 |
一般理论 | 2篇 |
预防医学 | 69篇 |
眼科学 | 28篇 |
药学 | 93篇 |
中国医学 | 7篇 |
肿瘤学 | 94篇 |
出版年
2023年 | 12篇 |
2022年 | 31篇 |
2021年 | 43篇 |
2020年 | 26篇 |
2019年 | 33篇 |
2018年 | 35篇 |
2017年 | 38篇 |
2016年 | 42篇 |
2015年 | 66篇 |
2014年 | 82篇 |
2013年 | 103篇 |
2012年 | 137篇 |
2011年 | 149篇 |
2010年 | 101篇 |
2009年 | 73篇 |
2008年 | 94篇 |
2007年 | 99篇 |
2006年 | 103篇 |
2005年 | 103篇 |
2004年 | 118篇 |
2003年 | 89篇 |
2002年 | 63篇 |
2001年 | 51篇 |
2000年 | 67篇 |
1999年 | 50篇 |
1998年 | 20篇 |
1997年 | 14篇 |
1996年 | 13篇 |
1995年 | 9篇 |
1994年 | 16篇 |
1993年 | 6篇 |
1992年 | 35篇 |
1991年 | 32篇 |
1990年 | 48篇 |
1989年 | 28篇 |
1988年 | 26篇 |
1987年 | 29篇 |
1986年 | 21篇 |
1985年 | 19篇 |
1984年 | 18篇 |
1983年 | 11篇 |
1982年 | 19篇 |
1981年 | 7篇 |
1979年 | 15篇 |
1978年 | 12篇 |
1977年 | 13篇 |
1975年 | 11篇 |
1973年 | 9篇 |
1972年 | 8篇 |
1969年 | 18篇 |
排序方式: 共有2298条查询结果,搜索用时 15 毫秒
991.
Claudia Erbelding-Denk Johannes Horst Schröder Manfred Schartl Indrajit Nanda Michael Schmid Jörg T. Epplen 《Behavior genetics》1994,24(1):95-101
The male-polymorphic poeciliid fish,Limia perugiae, a small teleostean endemic to the southeast of the Caribbean island Hispañola, consists of three male size morphs with uniform females. Large males differentiate at a size varying between 25 and 38 mm; intermediate males, between 21 and 25 mm. Under competition, large males exhibit an elaborate courship display, whereas small males show only a sneak-chase behavior. Intermediate males adapt their tactics to the respective competitors. However, all male morphs can switch from courtship display to sneak-chase behavior. In large mating groups with four males of different size and five or six virgin females, large dominant -males as well as small subordinate -males did not produce any offspring. Unexpectedly, all progeny were sired exclusively by the intemediate subordinate - and -males. Breeding experiments with the three male morphs can best be explained by a model of Y-linked genes for small and large size which are both suspended by the activity of an autosomal recessive repressor responsible for the development of intermediate males. The dominant allele of the recessive repressor, in either its homoorits heteozygous state, activates the Y-chromosomal genes for large or small size, respectively. Accordingly, intermediate males may produce male offspring of all size classes, depending on the presence of either the Y-linked gene or the autosomal repressor. 相似文献
992.
Kerkar N Ma Y Hussain M Muratori L Targett C Williams R Bianchi FB Mieli-Vergani G Vergani D 《Journal of immunological methods》1999,223(2):227-235
Liver Kidney Microsomal type 1 (LKM1) antibody, the diagnostic marker of autoimmune hepatitis type 2, is also found in a proportion of patients with hepatitis C virus infection (HCV). It is detected conventionally by the subjective immunofluorescence technique. Our aim was to establish a simple and objective enzyme-linked immunosorbent assay (ELISA) that measures antibodies to cytochrome P4502D6 (CYP2D6), the target of LKM1. An indirect ELISA using eukaryotically expressed CYP2D6 was designed. Absorbance values obtained against a reference microsomal preparation were subtracted from those obtained against a microsomal preparation over-expressing CYP2D6, thus removing the non-CYP2D6-specific reaction. Sera from 51 LKM1 positive patients (21 autoimmune hepatitis and 30 with HCV infection), 111 LKM1 negative patients with chronic liver disease (including 20 with HCV infection) and 43 healthy controls were tested. Of 51 patients positive by immunofluorescence, 48 were also positive by ELISA while all the 154 LKM1 negative subjects were also negative by ELISA. There was a high degree of association between IFL and ELISA as demonstrated by a kappa reliability value of 0.96. The absorbance values by ELISA correlated with immunofluorescence LKM1 titres both in autoimmune hepatitis (r = 0.74, p < 0.001) and HCV infection (r = 0.67, p < 0.001). The simple, objective ELISA described has the potential to replace the standard immunofluorescence technique. 相似文献
993.
Indrajit Nanda Sonja Schneider-Rasp Heinz Winking Michael Schmid 《Chromosome research》1995,3(7):399-409
Mouse chromosomes possessing multiple Robertsonian rearrangements (Rb chromosomes) have been examined using fluorescencein situ hybridization with the telomeric consensus sequence (TTAGGG)n. No hybridization signals were detected at the primary constriction of Rb chromosomes. This observation leads us to conclude that the formation of Rb chromosomes in the mouse is invariably associated with the loss of telomeric regions. More significantly, a further alteration in regions flanking the primary constrictions was observed after hybridizing with a minor satellite DNA probe to Rb chromosomes. It seems likely that the breakpoints required for a Robertsonian process do not include telomeric sites exclusively but extend to the adjacent pericentromeric regions of the original acrocentric chromosomes. In contrast to previous reports, these observations demonstrate the elimination of substantial amounts of chromosomal DNA during the formation of mouse Rb chromosomes. 相似文献
994.
Mulder C Schoonenboom NS Jansen EE Verhoeven NM van Kamp GJ Jakobs C Scheltens P 《Neuroscience letters》2005,386(2):69-71
Homocysteine accumulation, frequently observed in plasma of AD patients, may be a sign of a reduced activity of the brain methionine-homocysteine transmethylation cycle. S-Adenosylmethionine (SAM) is the main methyl donor in several transmethylation reactions. The demethylated product of SAM, S-adenosylhomocysteine (SAH), is hydrolyzed to yield homocysteine, which can be remethylated to methionine by transfer of a methyl group of 5-methyltetrahydrofolate (5-MTHF). A reduced activity of the transmethylation cycle in the brain may result in hypomethylation of the promoter of the presenilin 1 (PS1) gene, which will lead to overexpression of presenilin 1 and, consequently, to increased Abeta(1-42) (Abeta42) formation. Brain transmethylation was studied in 30 patients with 'probable' AD and 28 age-matched non-demented controls by measuring the cerebrospinal fluid (CSF) levels of SAM, SAH and 5-MTHF. 5-MTHF was determined by HPLC with electrochemical detection, while SAM and SAH were assayed by stable isotope dilution tandem mass spectrometry. We found no statistical differences between AD patients and controls for 5-MTHF, SAM and SAH levels, and the SAM/SAH-ratio in CSF. These findings argue against a possible change in methylation of the promoter and expression of PS1. 相似文献
995.
Alok K. Tembhurne Amita Maheshwari Himangi Warke Hemangi Chaudhari Shilpa C. Kerkar Kedar Deodhar Bharat Rekhi Jayanti Mania-Pramanik 《Journal of medical virology》2023,95(1):e27873
Killer cell immunoglobulin-like receptors (KIRs) are required for natural killer cell function against virus-infected cells or tumor cells. KIR gene content polymorphisms in Indian women with cervical cancer (CaCx) remain unexplored. Hence, we analyzed the frequencies of KIR genes, KIR haplotypes, and Bx subsets to draw their association with CaCx. The polymerase chain reaction-sequence-specific primer method was used for KIR genotyping in three groups of women: healthy controls (n = 114), women with human papillomavirus (HPV) infection (n = 70), and women with CaCx (n = 120). The results showed that the frequency of KIR2DS5 was significantly higher in women with CaCx compared to women with HPV infection (p = 0.02) and healthy controls (p = 0.01). Whereas the frequency of KIR2DL5B was significantly higher in healthy controls than in women with HPV infection (p = 0.02). The total number of activating KIR genes was higher in women with CaCx than in healthy controls (p = 0.006), indicating their positive association with CaCx. Moreover, the C4T4 subset was higher in women with CaCx than in women with HPV infection, though not significant. In conclusion, our findings highlight KIR2DS5, the C4T4 subset, and activating KIR genes are susceptible factors or positively associated with CaCx. Besides KIR2DL5B, this study also reported for the first time significantly high frequency of KIR2DL1 in healthy controls, indicating its possible protective association against CaCx. Further, significantly high frequency of KIR2DL3 observed in HPV-infected women might be also a promising biomarker for viral infections. Thus, the study confirms the association of KIR genes with cervical cancer in women with HPV infection. 相似文献
996.
997.
This report describes a case of cardiac rupture following acute myocardial infarction accurately diagnosed by transesophageal color Doppler echocardiography, which showed a very narrow transmural tract from the left ventricle into the pseudoaneurysm cavity. Such a small, slitlike rupture would most likely have been missed by an otherwise routine transesophageal examination, and we stress the importance of color Doppler interrogation in its accurate diagnosis. 相似文献
998.
LaMotte LC Nanda NC Thakur AC Agrawal G Kolda M 《Echocardiography (Mount Kisco, N.Y.)》1998,15(3):259-268
We describe the technique for identification of innominate veins, azygos vein, and left subclavian and internal jugular veins during transesophageal echocardiography. Validation was provided with contrast echocardiography. 相似文献
999.
Incidence of Red-Green colour blindness was studied in a Libyan population and was then compared with the same in two samples of Indian population. The incidence of the Red-Green colour defect was found to be 2.209% amongst the males and 0.0% amongst the females in the Libyan study. However, the incidence was only 1.841% amongst the ethnic Libyan males. This incidence was comparable with those reported from other African countries like Congo and Uganda. The incidence of Red-Green colour defect amongst the Indian males was found to be 2.295% while it was 0.840% amongst the Indian females. 相似文献
1000.
Lipoid proteinosis maps to 1q21 and is caused by mutations in the extracellular matrix protein 1 gene (ECM1) 总被引:12,自引:0,他引:12
Hamada T McLean WH Ramsay M Ashton GH Nanda A Jenkins T Edelstein I South AP Bleck O Wessagowit V Mallipeddi R Orchard GE Wan H Dopping-Hepenstal PJ Mellerio JE Whittock NV Munro CS van Steensel MA Steijlen PM Ni J Zhang L Hashimoto T Eady RA McGrath JA 《Human molecular genetics》2002,11(7):833-840
Lipoid proteinosis (LP), also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease (OMIM 247100) is a rare, autosomal recessive disorder typified by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline (glycoprotein) material and disruption/reduplication of basement membrane. The aetiology of LP is currently unknown. Using DNA from three affected siblings in a consanguineous Saudi Arabian family we performed genome-wide linkage and mapped the disorder to 1q21 (marker D1S498) with a two-point LOD score of 3.45 at theta = 0. A further 28 affected individuals from five other unrelated consanguineous family groups from different geographical regions also showed complete linkage and resulted in a maximum two-point LOD score of 21.85 at theta = 0. Using available markers in the interval between D1S442 and D1S305, the observed recombinants placed the gene in a 2.3 cM critical interval between D1S2344 and D1S2343 (Marshfield genetic map) corresponding to an approximately 6.5 Mb region on the UCSC physical map. Using a candidate gene approach (comparison of control versus LP gene expression in cultured fibroblasts) and subsequent direct sequencing of genomic DNA, we identified six different homozygous loss-of-function mutations in the extracellular matrix protein 1 gene (ECM1). Although the precise function of ECM1 is not known, our findings provide the first clinical indication of its relevance to skin adhesion, epidermal differentiation, wound healing, scarring, angiogenesis/angiopathy and basement membrane physiology, as well as defining the molecular basis of this inherited disorder. 相似文献