全文获取类型
收费全文 | 14885篇 |
免费 | 922篇 |
国内免费 | 70篇 |
专业分类
耳鼻咽喉 | 72篇 |
儿科学 | 549篇 |
妇产科学 | 360篇 |
基础医学 | 2077篇 |
口腔科学 | 254篇 |
临床医学 | 1465篇 |
内科学 | 3207篇 |
皮肤病学 | 370篇 |
神经病学 | 1485篇 |
特种医学 | 323篇 |
外科学 | 1595篇 |
综合类 | 85篇 |
一般理论 | 16篇 |
预防医学 | 1210篇 |
眼科学 | 299篇 |
药学 | 976篇 |
中国医学 | 25篇 |
肿瘤学 | 1509篇 |
出版年
2024年 | 19篇 |
2023年 | 163篇 |
2022年 | 280篇 |
2021年 | 516篇 |
2020年 | 313篇 |
2019年 | 437篇 |
2018年 | 482篇 |
2017年 | 395篇 |
2016年 | 391篇 |
2015年 | 486篇 |
2014年 | 628篇 |
2013年 | 810篇 |
2012年 | 1265篇 |
2011年 | 1173篇 |
2010年 | 696篇 |
2009年 | 585篇 |
2008年 | 996篇 |
2007年 | 998篇 |
2006年 | 954篇 |
2005年 | 869篇 |
2004年 | 836篇 |
2003年 | 777篇 |
2002年 | 623篇 |
2001年 | 84篇 |
2000年 | 70篇 |
1999年 | 99篇 |
1998年 | 113篇 |
1997年 | 99篇 |
1996年 | 86篇 |
1995年 | 66篇 |
1994年 | 53篇 |
1993年 | 59篇 |
1992年 | 52篇 |
1991年 | 45篇 |
1990年 | 28篇 |
1989年 | 31篇 |
1988年 | 30篇 |
1987年 | 26篇 |
1986年 | 21篇 |
1985年 | 33篇 |
1984年 | 29篇 |
1983年 | 20篇 |
1982年 | 20篇 |
1981年 | 11篇 |
1980年 | 16篇 |
1979年 | 11篇 |
1978年 | 10篇 |
1977年 | 10篇 |
1972年 | 8篇 |
1971年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
A series of murine leukemia viruses (MuLVs) with chimeric envelope proteins (Env) was generated to map functional interactions between the N- and the C-terminal domains of surface proteins (SU). All these chimeras have the 4070A amphotropic receptor-binding region flanked by various lengths of Moloney ecotropic N- and C-terminal Env. A charged residue, E49 (E16 on the mature protein), was identified at the N-terminals of Moloney MuLV SU that is important for the interaction with the C-terminal domain of the SU. The region that interacts with E49 was localized between junction 4 (R265 of M-MuLV Env) and junction 6 (L374 of M-MuLV Env) of SU. Sequencing the viable chimeric Env virus populations identified residues within the SU protein that improved the replication kinetics of the input chimeric Env viruses. Mutations in the C-domain of SU (G387E/R, L435I, L442P) were found to improve chimera IV4, which displayed a delayed onset of replication. The replication of AE6, containing a chimeric junction in the SU C-terminus, was improved by mutations in the N-domain (N40H, E80K), the proline-rich region (Q252R), or the transmembrane protein (L538N). Altogether, these observations provide insights into the structural elements required for Env function. 相似文献
92.
Schillerstrom JE Horton MS Schillerstrom TL Joshi KG Earthman BS Velez AM Royall DR 《Psychosomatics》2005,46(5):411-417
The purpose of this study was to determine the prevalence, course, and risk factors for executive impairment in patients hospitalized on a general medicine service. One hundred patients were administered the Executive Interview (EXIT25), the Executive Clock Drawing Task (CLOX), and the Mini-Mental State Examination at admission and discharge. Fifty-two percent of the patients at admission and 56% at discharge had scores indicating impairment on at least one measure of executive function. Median scores on every measure improved during hospitalization. Older patients and those with a cardiac or gastrointestinal disorder were more likely to have executive impairment. The prevalence of executive impairment on general medicine services is high. Although improvement in executive function occurs during hospitalization, many patients remained impaired. 相似文献
93.
94.
95.
Carlo M. Crocem Monica Shander Joanne Martinis Lucienne Cicurel Gian G. D'ancona Hilary Koprowski 《European journal of immunology》1980,10(6):486-488
Loss of human chromosomes from mouse × human hybridomas is not random. Human chromosomes 14, 5 and 22 are preferentially retained, while chromosomes 2 and 1 are preferentially lost. Interestingly, human chromosome 14, which carries the genes for human immunoglobulin heavy chains, appears to be retained by almost all the hybrid clones and subclones. 相似文献
96.
Decrease and gain of gene expression are equally discriminatory markers for prostate carcinoma: a gene expression analysis on total and microdissected prostate tissue 总被引:8,自引:0,他引:8
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
97.
Marta Zelazko Magda Carneiro-Sampaio Monica Cornejo De Luigi Diana Garcia De Olarte Oscar Porras Madrigal Renato Berrón Perez Agueda Cabello Marylin Valentin Rostan Ricardo U. Sorensen 《Journal of clinical immunology》1998,18(2):161-166
The Latin American Group for Primary Immunodeficiencies, formed in 1993, presently includes 12 countries. One goal was to study the frequency of primary immunodeficiencies in various regions of the American continent and to enhance knowledge about these diseases among primary-care physicians, as well as allergist–immunologists. Important for this purpose was the development of a registry of primary immunodeficiencies using a uniform questionnaire and computerized database. To date, eight countries have collected information on a total of 1428 patients. Predominantly antibody deficiencies were reported in 58% of patients, followed by cellular and antibody immunodeficiencies associated with other abnormalities in 18%, immunodeficiency syndromes associated with granulocyte dysfunction in 8%, phagocytic disorders in 9%, combined cellular and antibody immunodeficiencies in 5%, and complement deficiencies in 2% of patients. The information gathered from this initial analysis of data will serve to expand the patient database to more areas within participating countries and to new countries and to increase collaboration toward better diagnosis and treatment of these diseases. 相似文献
98.
Zaffe D Rodriguez Y Baena R Rizzo S Brusotti C Soncini M Pietrabissa R Cavani F Quaglini V 《Journal of biomedical materials research. Part A》2003,64(2):365-371
Fourteen titanium dental implants (Tioblast) were implanted singly in the proximal tibia of New Zealand rabbits for 120 days. A bone defect was surgically produced and filled with Bio-Oss around six of these implants. After the animals were sacrificed and their organs harvested, bone segments were fixed and methacrylate embedded after the push-in test had been performed. Microradiography was performed on longitudinal sections of the implants, whereas scanning electron microscope analysis was performed on the remaining embedded half-implants using secondary electrons only. The results showed that the implants were apically and coronally surrounded by bone, whether Bio-Oss was used or not. Fractures were evident through the newly formed bone and between the pre-existing and newly formed bone. Some fracture lines propagated through the bone and stopped at the implant surface without continuing along the bone-titanium interface. Detachment between the implant and the bone occurred at the coronal extremity of the implants and along its cervical region. These results highlight the fact that the bone-titanium interface has a high resistance to loading. It exhibited greater resistance than the newly formed bone and seems to behave in a manner similar to the cement lines of osteons. 相似文献
99.
Alteration of the LRP1B gene region is associated with high grade of urothelial cancer 总被引:6,自引:0,他引:6
Langbein S Szakacs O Wilhelm M Sukosd F Weber S Jauch A Lopez Beltran A Alken P Kälble T Kovacs G 《Laboratory investigation; a journal of technical methods and pathology》2002,82(5):639-643
We have delineated regions of interest at chromosome 2q21.2, 2q36.3, and 2q37.1 by deletion mapping of 114 urothelial cancers (UC). Altogether, 17%, 18%, and 63% of the G1, G2, and G3 tumors displayed loss of heterozygosity at chromosome 2q, respectively, The region at 2q21.2 was narrowed down to the LRP1B gene (NT_005129.6). Hemi- and homozygous deletion at the LRP1B gene region was seen in 31 of 114 UCs. Only 8% of the UCs with G1 and none with G2 tumors showed loss of heterozygosity at the LRP1B gene, whereas 49% of the G3 UCs had allelic loss at this region. RT-PCR analysis of the LRP1B gene showed the lack of expression of several exons in 2 of 9 cases analyzed. Our analysis suggests that the LRP1B gene is a candidate tumor suppressor gene in UCs. 相似文献
100.
Plewako H Arvidsson M Petruson K Oancea I Holmberg K Adelroth E Gustafsson H Sandström T Rak S 《The Journal of allergy and clinical immunology》2002,110(1):68-71
BACKGROUND: In sensitized patients, coupling between IgE and FcepsilonRI receptors on mast cells leads to release of proinflammatory mediators and a subsequent influx of inflammatory cells to the affected organ. Omalizumab (Xolair; formerly rhuMAb-E25) binds to circulating IgE, thus preventing induction of the allergic process. OBJECTIVE: We investigated the effect of treatment with omalizumab on seasonal allergic rhinitis and related changes in inflammatory cell numbers in nasal biopsy specimens. METHODS: Patients were randomized to treatment with omalizumab or placebo before the pollen season; the treatment was started and continued during season. Symptoms and use of medication were recorded, and blood samples and nasal biopsy specimens were obtained before and during season. Immunocytochemistry was performed on biopsy sections through use of the following antibodies: anti-CD4, CD8 (T lymphocytes), EG2, and anti-eosinophil peroxidase (eosinophils), anti-tryptase (mast cells), human neutrophil lipocalin (neutrophils), and antibodies against IgE and FcepsilonRI. RESULTS: During the season, blood eosinophils increased in placebo-treated patients but not in omalizumab-treated patients (P =.01); the difference between the treatment groups was significant (P =.04). Free IgE in serum decreased significantly (P =.0002) in omalizumab-treated patients but not in placebo-treated patients; the difference between the groups was significant (P =.0001). In nasal biopsy specimens, the number of eosinophil peroxidase-positive staining cells increased in the placebo-treated patients (P =.003) but not in the actively treated patients during the season; the difference between the groups was significant (P =.0001). The number of IgE(+) staining cells decreased significantly in the omalizumab group during the season in comparison with the placebo group (P =.04). CONCLUSION: The clinical benefit of treatment with omalizumab is associated with an anti-inflammatory effect on cellular markers in blood and nasal tissue. 相似文献