全文获取类型
收费全文 | 131篇 |
免费 | 3篇 |
国内免费 | 9篇 |
专业分类
儿科学 | 7篇 |
妇产科学 | 1篇 |
基础医学 | 20篇 |
口腔科学 | 4篇 |
临床医学 | 17篇 |
内科学 | 20篇 |
皮肤病学 | 4篇 |
特种医学 | 36篇 |
外科学 | 10篇 |
综合类 | 13篇 |
预防医学 | 6篇 |
药学 | 3篇 |
肿瘤学 | 2篇 |
出版年
2022年 | 2篇 |
2021年 | 2篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 5篇 |
2012年 | 5篇 |
2011年 | 7篇 |
2010年 | 7篇 |
2009年 | 4篇 |
2008年 | 1篇 |
2007年 | 5篇 |
2006年 | 1篇 |
2005年 | 4篇 |
2004年 | 3篇 |
2003年 | 4篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1998年 | 7篇 |
1997年 | 12篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 6篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1943年 | 1篇 |
排序方式: 共有143条查询结果,搜索用时 0 毫秒
41.
42.
43.
SUMMARY: Recent studies have highlighted the intrinsic toxicity of filtered proteins and their contribution to deterioration of renal function. In-depth analysis of proteinuria may assess the degree of glomerular and/or tubular involvement and help in the prognostication of an individual patient. One should consider not only the quantity but also the quality of the urinary protein. Apart from quantitating the total urinary protein, assessment of the quality of the protein may be achieved by performing sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) for an overview assessment follow by automated nephelometry or simple radial immunodiffusion measurement of specific proteins for calculating the selectivity index and detecting tubular dysfunction. 相似文献
44.
45.
Quinn KT Ng Marie K Sutton Pan Soonsawad Li Xing Holland Cheng Tatiana Segura 《Molecular therapy》2009,17(5):828-836
The development of techniques to efficiently deliver genes using nonviral approaches can broaden the application of gene delivery in medical applications without the safety concerns associated with viral vectors. Here, we designed a clustered integrin-binding platform to enhance the efficiency and targetability of nonviral gene transfer to HeLa cells with low and high densities of αvβ3 integrin receptors. Arg-Gly-Asp (RGD) nanoclusters were formed using gold nanoparticles functionalized with RGD peptides and used to modify the surface of DNA/poly(ethylene imine) (PEI) polyplexes. DNA/PEI polyplexes with attached RGD nanoclusters resulted in either 5.4- or 35-fold increase in gene transfer efficiency over unmodified polyplexes for HeLa cells with low- or high-integrin surface density, respectively. The transfection efficiency obtained with the commercially available vector jetPEI-RGD was used for comparison as a vector without clustered binding. JetPEI-RGD exhibited a 1.2-fold enhancement compared to unmodified jetPEI in cells with high densities of αvβ3 integrin receptors. The data presented here emphasize the importance of the RGD conformational arrangement on the surface of the polyplex to achieve efficient targeting and gene transfer, and provide an approach to introduce clustering to a wide variety of nanoparticles for gene delivery. 相似文献
46.
47.
48.
Ames试验研究鞣酸和相关化合物的毒性和抗突变性 总被引:2,自引:0,他引:2
[目的 ]Ames试验研究鞣酸和相关化合物抗突变作用。 [方法 ]应用平皿掺入和悬浮培养抗突变性方法 ,比较研究鞣酸和相关化合物在无抑制和抑制浓度减少 2 硝基芴 (2 NF)、4 硝基喹啉 1 氧化物 (4 NQO)和N 甲基 N 硝基 N 亚硝基胍 (MNNG)回复菌落数。 [结果 ]鞣酸和相关化合物月桂木立口 酸、鞣花酸、木立口 酸丙脂、木立口 酸甲脂、木立口 酸和儿茶素抑制Ames试验沙门氏菌菌株生长 ,这种抑制作用与减少Ames试验回复突变和诱发突变菌落数相关。应用悬浮培养方法 ,鞣酸和相关化合物在无抑制浓度和抑制浓度对 2 NF、4 NQO和MNNG无抗突变作用。 [结论 ]Ames试验悬浮培养法是研究抑菌剂类化合物抗突变有用方法 ,可区别由毒性和抗突变作用减少的回复菌落数。 相似文献
49.
Lotery AJ; Ennis KT; Silvestri G; Nicholl S; McGibbon D; Collins AD; Hughes AE 《Human molecular genetics》1996,5(5):705-708
Central areolar choroidal dystrophy (CACD) is a rare inherited retinal
disease which causes progressive profound loss of vision in patients during
their 4th decade. We have identified a Northern Irish family with 19
affected individuals in three living generations. We have performed a total
genome search and established linkage of CACD in this family to chromosome
17p (multipoint Zmax = 5.65 at D17S938). The genes for phosphatidylinositol
transfer protein (PITPN), retinal guanylate cyclase (GUC2D), beta-arrestin
2 (ARRB2), pigment epithelium-derived factor (PEDF) and recoverin (RCV1)
map to this region and are candidate genes for retinal disease. Analysis of
the coding region of the PITPN gene failed to reveal any mutation in this
family.
相似文献
50.
The purpose of the study was to investigate the peak plantar pressure (PP) and kinematic adjustments to various negative gradients (−GR) and walking velocities (WV) during treadmill fitness walking. While filmed, plantar pressure data for seven sensors were collected for eight female subjects. Faster WV were associated with increased heel PP (P < 0.05), but decreased forefoot PP. During −GR, subjects exhibited increased fifth metatarsal PP, but decreased medial forefoot PP. Greater knee flexion and step length respectively were inversely and directly related to heel PP. These heel PP responses were related to individual subject adaptations to −GR. 相似文献